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P47025 · MDV1_YEAST

Function

function

Involved in mitochondrial fission. Has a partially redundant function to CAF4 in acting as an adapter protein, binding to FIS1 on the mitochondrial outer membrane and recruiting the dynamin-like GTPase DNM1 to form mitochondrial fission complexes. Formation of these complexes is required to promote constriction and fission of the mitochondrial compartment at a late step in mitochondrial division.

Miscellaneous

Present with 3730 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmitochondrial outer membrane
Cellular Componentmitochondrion
Molecular Functionubiquitin binding
Biological Processmitochondrial fission
Biological Processmitochondrial genome maintenance
Biological Processperoxisome fission
Biological Processpositive regulation of mitochondrial fission

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Mitochondrial division protein 1
  • Alternative names
    • Mitochondria fission 2 protein

Gene names

    • Name
      MDV1
    • Synonyms
      FIS2, GAG3, NET2
    • ORF names
      J0802
    • Ordered locus names
      YJL112W

Organism names

Accessions

  • Primary accession
    P47025
  • Secondary accessions
    • D6VW72

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion outer membrane
; Peripheral membrane protein
Note: Uniformly distributed on the cytoplasmic face of the mitochondrial outer membrane. This localization is dependent on FIS1. Reorganizes to punctate structures on mitochondria, corresponding to mitochondrial constriction sites, at a late step in mitochondrial division. This relocalization is dependent on DNM1.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis148Abolishes interaction with FIS1.
Mutagenesis250Suppresses the mitochondrial fission defect of a FIS1-3 mutant by affecting interaction with FIS1.
Mutagenesis400No interaction with FIS1; slight decrease in interaction with DNM1.
Mutagenesis440Abolishes interaction with DNM1.
Mutagenesis461Slight decrease in interaction with FIS1; 20-fold decrease in interaction with DNM1; even distribution along mitochondria not punctate.
Mutagenesis544Results in mitochondrial division defect, but has no effect on the interaction with DNM1.
Mutagenesis605Abolishes interaction with DNM1.
Mutagenesis664Slight decrease in interaction with DNM1.
Mutagenesis689Slight decrease in interaction with DNM1.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 17 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00000514771-714Mitochondrial division protein 1
Modified residue376Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with CAF4, DNM1 and FIS1, components of the mitochondrial fission machinery. Interacts via its N-terminal, coiled-coil extension (NTE) with FIS1, and via its WD repeats with DNM1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY P47025FIS1 P405155EBI-26032, EBI-25059

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for coiled coil, region, compositional bias, repeat.

Type
IDPosition(s)Description
Coiled coil240-298
Region323-354Disordered
Compositional bias330-347Polar residues
Repeat396-436WD 1
Repeat439-478WD 2
Repeat500-539WD 3
Repeat561-603WD 4
Repeat604-642WD 5
Repeat644-681WD 6
Repeat685-714WD 7

Sequence similarities

Belongs to the WD repeat MDV1/CAF4 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    714
  • Mass (Da)
    80,032
  • Last updated
    1996-02-01 v1
  • MD5 Checksum
    E4BDD8DF803B295F0DFC24503C06ED2F
MSVNDQITHIGKTLSTTASAFLNYQKSNSNTQDVLTNNGPYKNLLSNTVNNASSTSYFYKRTEHGRFVKNASNTFEDIYSKTRRGDVFRNKFTDNKTCFRMLTYISDDLLNEIPTKEGLKSDADGKLLTEGGENENLRKNASKKETSLFQGFKSYLPIAELAIENTERLNYDTNGTSGTVGAKDVMSKTNERDEIHTELPNFQDSFLIPPGVETKKISSSYSPSALKSFSQTLVNSLEFLNIQKNSTLSEIRDIEVEVENLRQKKEKLLGKIANIEQNQLLLEDNLKQIDDRLDFLEEYGLEVIEANSDENAEDDGMSERKALKNDAIRNEGVTTESISSEASNLPPRRRQQLRDDNSLNRLGAFYSKSKKRHRKSFPTFQQLYEPGTKIGSIMSTHDDFLTCLDFDAPFGTLCTAGYLDHTVKIWDLSKQNKIGELAGHLATINCMQINRDYGTLVTGGRDAALKLWNLNLAQQLYQETQNLTSPTNHIDSPCVHTFEAHTDEVTALSLDPSFLVSGSQDRTIRQWDLRSGKCLQTIDLSFANVLTTSTNVDLSKSTLLTQRNERPSIGALQSFDAALATGTKDGVVRLWDLRSGKVIRTLKGHTDAITSLKFDSACLVTGSYDRTVRIWDLRTGLLNKFHAYSAPVLSLDLFQENAAVVVADEPSVQIYDSEKDESWSCVEQGNETSVSTVKYKENYMVEGRENGDVNIWAV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias330-347Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z49387
EMBL· GenBank· DDBJ
CAA89407.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006943
EMBL· GenBank· DDBJ
DAA08688.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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