P46948 · RRP41_YEAST

Function

function

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. SKI6 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation.

Miscellaneous

Present with 5150 molecules/cell in log phase SD medium.

Caution

Was originally thought to have exonuclease activity but it was later shown (PubMed:17173052, PubMed:17174896) that only DIS3/RRP44 subunit of the exosome core has this activity.

GO annotations

AspectTerm
Cellular Componentcytoplasmic exosome (RNase complex)
Cellular Componentexosome (RNase complex)
Cellular Componentnuclear exosome (RNase complex)
Cellular Componentnucleolus
Cellular Componentnucleus
Molecular FunctionRNA binding
Biological Processexonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Biological Processnuclear mRNA surveillance
Biological Processnuclear polyadenylation-dependent CUT catabolic process
Biological Processnuclear-transcribed mRNA catabolic process
Biological Processnuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay
Biological Processpoly(A)-dependent snoRNA 3'-end processing
Biological ProcessRNA catabolic process
Biological ProcessRNA processing
Biological ProcessrRNA catabolic process
Biological ProcessTRAMP-dependent tRNA surveillance pathway
Biological ProcessU1 snRNA 3'-end processing
Biological ProcessU4 snRNA 3'-end processing
Biological ProcessU5 snRNA 3'-end processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Exosome complex component SKI6
  • Alternative names
    • Extracellular mutant protein 20
    • Ribosomal RNA-processing protein 41
    • Superkiller protein 6

Gene names

    • Name
      SKI6
    • Synonyms
      ECM20, RRP41
    • ORF names
      G7587
    • Ordered locus names
      YGR195W

Organism names

Accessions

  • Primary accession
    P46948
  • Secondary accessions
    • D6VUX7

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis62-63Impairs RNA-binding (at the proposed ring entry site).
Mutagenesis95-96Impairs RNA-binding (at the proposed ring exit site).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001399621-246Exosome complex component SKI6

Proteomic databases

PTM databases

Interaction

Subunit

Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which associates with catalytic subunits DIS3 and RRP6 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits and peripheral S1 domain-containing components CSL4, RRP4 and RRP40 located on the top of the ring structure.

Binary interactions

View interactors in UniProtKB
View CPX-599 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Sequence similarities

Belongs to the RNase PH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    246
  • Mass (Da)
    27,561
  • Last updated
    1995-11-01 v1
  • Checksum
    C80EFFB528A43044
MSRLEIYSPEGLRLDGRRWNELRRFESSINTHPHAADGSSYMEQGNNKIITLVKGPKEPRLKSQMDTSKALLNVSVNITKFSKFERSKSSHKNERRVLEIQTSLVRMFEKNVMLNIYPRTVIDIEIHVLEQDGGIMGSLINGITLALIDAGISMFDYISGISVGLYDTTPLLDTNSLEENAMSTVTLGVVGKSEKLSLLLVEDKIPLDRLENVLAIGIAGAHRVRDLMDEELRKHAQKRVSNASAR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X82775
EMBL· GenBank· DDBJ
CAA58018.1
EMBL· GenBank· DDBJ
Genomic DNA
Z72980
EMBL· GenBank· DDBJ
CAA97221.1
EMBL· GenBank· DDBJ
Genomic DNA
Z72981
EMBL· GenBank· DDBJ
CAA97223.1
EMBL· GenBank· DDBJ
Genomic DNA
AY558509
EMBL· GenBank· DDBJ
AAS56835.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA08288.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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