P46948 · RRP41_YEAST
- ProteinExosome complex component SKI6
- GeneSKI6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids246 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. SKI6 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation.
Miscellaneous
Present with 5150 molecules/cell in log phase SD medium.
GO annotations
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameExosome complex component SKI6
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP46948
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 62-63 | Impairs RNA-binding (at the proposed ring entry site). | ||||
Sequence: KS → ED | ||||||
Mutagenesis | 95-96 | Impairs RNA-binding (at the proposed ring exit site). | ||||
Sequence: RR → EE |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000139962 | 1-246 | Exosome complex component SKI6 | |||
Sequence: MSRLEIYSPEGLRLDGRRWNELRRFESSINTHPHAADGSSYMEQGNNKIITLVKGPKEPRLKSQMDTSKALLNVSVNITKFSKFERSKSSHKNERRVLEIQTSLVRMFEKNVMLNIYPRTVIDIEIHVLEQDGGIMGSLINGITLALIDAGISMFDYISGISVGLYDTTPLLDTNSLEENAMSTVTLGVVGKSEKLSLLLVEDKIPLDRLENVLAIGIAGAHRVRDLMDEELRKHAQKRVSNASAR |
Proteomic databases
PTM databases
Interaction
Subunit
Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which associates with catalytic subunits DIS3 and RRP6 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits and peripheral S1 domain-containing components CSL4, RRP4 and RRP40 located on the top of the ring structure.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P46948 | CSL4 P53859 | 18 | EBI-1788, EBI-1731 | |
BINARY | P46948 | DIS3 Q08162 | 10 | EBI-1788, EBI-1740 | |
BINARY | P46948 | MTR3 P48240 | 9 | EBI-1788, EBI-1749 | |
BINARY | P46948 | RRP4 P38792 | 5 | EBI-1788, EBI-1757 | |
BINARY | P46948 | RRP40 Q08285 | 5 | EBI-1788, EBI-1831 | |
BINARY | P46948 | RRP42 Q12277 | 5 | EBI-1788, EBI-1765 | |
BINARY | P46948 | RRP43 P25359 | 5 | EBI-1788, EBI-1773 | |
BINARY | P46948 | RRP45 Q05636 | 12 | EBI-1788, EBI-1810 | |
BINARY | P46948 | RRP46 P53256 | 7 | EBI-1788, EBI-1842 | |
BINARY | P46948 | RRP6 Q12149 | 4 | EBI-1788, EBI-1782 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length246
- Mass (Da)27,561
- Last updated1995-11-01 v1
- ChecksumC80EFFB528A43044
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X82775 EMBL· GenBank· DDBJ | CAA58018.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72980 EMBL· GenBank· DDBJ | CAA97221.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72981 EMBL· GenBank· DDBJ | CAA97223.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY558509 EMBL· GenBank· DDBJ | AAS56835.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA08288.1 EMBL· GenBank· DDBJ | Genomic DNA |