P46943 · GUF1_YEAST

Function

function

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.
This protein may be expected to contain an N-terminal transit peptide but none has been predicted.

Catalytic activity

Features

Showing features for binding site.

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TypeIDPosition(s)Description
Binding site53-60GTP (UniProtKB | ChEBI)
Binding site120-124GTP (UniProtKB | ChEBI)
Binding site174-177GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial inner membrane
Cellular Componentmitochondrial matrix
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmitochondrial ribosome binding
Biological Processpositive regulation of translation
Biological Processtranslation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Translation factor GUF1, mitochondrial
  • EC number
  • Alternative names
    • Elongation factor 4 homolog
      (EF-4
      )
    • GTPase GUF1
    • Ribosomal back-translocase

Gene names

    • Name
      GUF1
    • ORF names
      L8003.7
    • Ordered locus names
      YLR289W

Organism names

Accessions

  • Primary accession
    P46943
  • Secondary accessions
    • D6VYT5
    • Q05891

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion inner membrane
; Peripheral membrane protein

Keywords

Phenotypes & Variants

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000915601-645Translation factor GUF1, mitochondrial

Proteomic databases

Interaction

Protein-protein interaction databases

Chemistry

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain44-228tr-type G

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    645
  • Mass (Da)
    73,188
  • Last updated
    1997-11-01 v2
  • Checksum
    BA1C371CCFECDC9B
MLKFRIRPVRHIRCYKRHAYFLRYNHTTTPAQKLQAQIEQIPLENYRNFSIVAHVDHGKSTLSDRLLEITHVIDPNARNKQVLDKLEVERERGITIKAQTCSMFYKDKRTGKNYLLHLIDTPGHVDFRGEVSRSYASCGGAILLVDASQGIQAQTVANFYLAFSLGLKLIPVINKIDLNFTDVKQVKDQIVNNFELPEEDIIGVSAKTGLNVEELLLPAIIDRIPPPTGRPDKPFRALLVDSWYDAYLGAVLLVNIVDGSVRKNDKVICAQTKEKYEVKDIGIMYPDRTSTGTLKTGQVGYLVLGMKDSKEAKIGDTIMHLSKVNETEVLPGFEEQKPMVFVGAFPADGIEFKAMDDDMSRLVLNDRSVTLERETSNALGQGWRLGFLGSLHASVFRERLEKEYGSKLIITQPTVPYLVEFTDGKKKLITNPDEFPDGATKRVNVAAFHEPFIEAVMTLPQEYLGSVIRLCDSNRGEQIDITYLNTNGQVMLKYYLPLSHLVDDFFGKLKSVSRGFASLDYEDAGYRISDVVKLQLLVNGNAIDALSRVLHKSEVERVGREWVKKFKEYVKSQLYEVVIQARANNKIIARETIKARRKDVLQKLHASDVSRRKKLLAKQKEGKKHMKTVGNIQINQEAYQAFLRR

Sequence caution

The sequence AAA96351.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict260in Ref. 1; AAA96351
Sequence conflict354in Ref. 1; AAA96351
Sequence conflict374in Ref. 1; AAA96351
Sequence conflict559in Ref. 1; AAA96351

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U22360
EMBL· GenBank· DDBJ
AAA96351.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
U17243
EMBL· GenBank· DDBJ
AAB67335.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006945
EMBL· GenBank· DDBJ
DAA09601.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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