P46644 · AAT3_ARATH
- ProteinAspartate aminotransferase 3, chloroplastic
- GeneASP3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids449 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activity
- 2-oxoglutarate + L-aspartate = L-glutamate + oxaloacetate
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.5 mM | L-aspartate |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | cytosol | |
Cellular Component | peroxisome | |
Cellular Component | plasma membrane | |
Cellular Component | plastid | |
Molecular Function | L-aspartate:2-oxoglutarate aminotransferase activity | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | 2-oxoglutarate metabolic process | |
Biological Process | aspartate metabolic process | |
Biological Process | biosynthetic process | |
Biological Process | glutamate metabolic process | |
Biological Process | leaf senescence |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAspartate aminotransferase 3, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP46644
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: accumulates in stromules, which are membrane-bound protrusions of the plastid envelope containing soluble stroma. Stromules are often found connecting plastids within a cell.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for transit peptide, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-43 | Chloroplast | ||||
Sequence: MKTTHFSSSSSSDRRIGALLRHLNSGSDSDNLSSLYASPTSGG | ||||||
Chain | PRO_0000001210 | 44-449 | Aspartate aminotransferase 3, chloroplastic | |||
Sequence: TGGSVFSHLVQAPEDPILGVTVAYNKDPSPVKLNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRTRIKEYLPIVGLVEFNKLSAKLILGADSPAIRENRITTVECLSGTGSLRVGGEFLAKHYHQKTIYITQPTWGNHPKIFTLAGLTVKTYRYYDPATRGLNFQGLLEDLGAAAPGSIVLLHACAHNPTGVDPTIQQWEQIRKLMRSKGLMPFFDSAYQGFASGSLDTDAKPIRMFVADGGECLVAQSYAKNMGLYGERVGALSIVCKSADVAGRVESQLKLVIRPMYSSPPIHGASIVAVILRDKNLFNEWTLELKAMADRIISMRKQLFEALRTRGTPGDWSHIIKQIGMFTFTGLNPAQVSFMTKEYHIYMTSDGRISMAGLSSKTVPHLADAIHAVVTKAV | ||||||
Modified residue | 295 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in roots, cauline leaves, flowers, hypocotyl epidermis and root hair cells.
Induction
By ethylene and dark.
Developmental stage
Up-regulated in leaves during natural senescence.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length449
- Mass (Da)48,954
- Last updated1995-11-01 v1
- Checksum8668EA40367C6A58
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U15034 EMBL· GenBank· DDBJ | AAA79371.1 EMBL· GenBank· DDBJ | mRNA | ||
AL163815 EMBL· GenBank· DDBJ | CAB87712.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED91691.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY050765 EMBL· GenBank· DDBJ | AAK92700.1 EMBL· GenBank· DDBJ | mRNA | ||
AY079310 EMBL· GenBank· DDBJ | AAL85041.1 EMBL· GenBank· DDBJ | mRNA | ||
AB047807 EMBL· GenBank· DDBJ | BAB32884.1 EMBL· GenBank· DDBJ | mRNA |