P46644 · AAT3_ARATH

Function

function

Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
2.5 mML-aspartate

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site81L-aspartate (UniProtKB | ChEBI)
Binding site178L-aspartate (UniProtKB | ChEBI)
Binding site231L-aspartate (UniProtKB | ChEBI)
Binding site423L-aspartate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentcytosol
Cellular Componentperoxisome
Cellular Componentplasma membrane
Cellular Componentplastid
Molecular FunctionL-aspartate:2-oxoglutarate aminotransferase activity
Molecular Functionpyridoxal phosphate binding
Biological Process2-oxoglutarate metabolic process
Biological Processaspartate metabolic process
Biological Processbiosynthetic process
Biological Processglutamate metabolic process
Biological Processleaf senescence

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aspartate aminotransferase 3, chloroplastic
  • EC number
  • Alternative names
    • Protein YELLOW-LEAF-SPECIFIC GENE 4
    • Transaminase A

Gene names

    • Name
      ASP3
    • Synonyms
      YLS4
    • ORF names
      F15N18.110
    • Ordered locus names
      At5g11520

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P46644
  • Secondary accessions
    • Q9CAZ5

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Plastid, chloroplast
Note: accumulates in stromules, which are membrane-bound protrusions of the plastid envelope containing soluble stroma. Stromules are often found connecting plastids within a cell.

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain, modified residue.

TypeIDPosition(s)Description
Transit peptide1-43Chloroplast
ChainPRO_000000121044-449Aspartate aminotransferase 3, chloroplastic
Modified residue295N6-(pyridoxal phosphate)lysine

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in roots, cauline leaves, flowers, hypocotyl epidermis and root hair cells.

Induction

By ethylene and dark.

Developmental stage

Up-regulated in leaves during natural senescence.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    449
  • Mass (Da)
    48,954
  • Last updated
    1995-11-01 v1
  • Checksum
    8668EA40367C6A58
MKTTHFSSSSSSDRRIGALLRHLNSGSDSDNLSSLYASPTSGGTGGSVFSHLVQAPEDPILGVTVAYNKDPSPVKLNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRTRIKEYLPIVGLVEFNKLSAKLILGADSPAIRENRITTVECLSGTGSLRVGGEFLAKHYHQKTIYITQPTWGNHPKIFTLAGLTVKTYRYYDPATRGLNFQGLLEDLGAAAPGSIVLLHACAHNPTGVDPTIQQWEQIRKLMRSKGLMPFFDSAYQGFASGSLDTDAKPIRMFVADGGECLVAQSYAKNMGLYGERVGALSIVCKSADVAGRVESQLKLVIRPMYSSPPIHGASIVAVILRDKNLFNEWTLELKAMADRIISMRKQLFEALRTRGTPGDWSHIIKQIGMFTFTGLNPAQVSFMTKEYHIYMTSDGRISMAGLSSKTVPHLADAIHAVVTKAV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U15034
EMBL· GenBank· DDBJ
AAA79371.1
EMBL· GenBank· DDBJ
mRNA
AL163815
EMBL· GenBank· DDBJ
CAB87712.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED91691.1
EMBL· GenBank· DDBJ
Genomic DNA
AY050765
EMBL· GenBank· DDBJ
AAK92700.1
EMBL· GenBank· DDBJ
mRNA
AY079310
EMBL· GenBank· DDBJ
AAL85041.1
EMBL· GenBank· DDBJ
mRNA
AB047807
EMBL· GenBank· DDBJ
BAB32884.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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