P46103 · DRTS_PLAVN
- ProteinBifunctional dihydrofolate reductase-thymidylate synthase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids182 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).
Catalytic activity
- (6S)-5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+
Pathway
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 25-31 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 40 | substrate | |||
Binding site | 93-95 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 114-117 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 154 | substrate | |||
Binding site | 155-162 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 160 | substrate | |||
Binding site | 175 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Molecular Function | dihydrofolate reductase activity | |
Molecular Function | NADP binding | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dihydrofolate metabolic process | |
Biological Process | folic acid metabolic process | |
Biological Process | methylation | |
Biological Process | nucleotide biosynthetic process | |
Biological Process | one-carbon metabolic process | |
Biological Process | tetrahydrofolate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional dihydrofolate reductase-thymidylate synthase
- Short namesDHFR-TS
Including 2 domains:
- Recommended nameDihydrofolate reductase
- EC number
- Recommended nameThymidylate synthase
- EC number
Organism names
- Organism
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Aconoidasida > Haemosporida > Plasmodiidae > Plasmodium > Plasmodium (Vinckeia)
Accessions
- Primary accessionP46103
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000186350 | 1-182 | Bifunctional dihydrofolate reductase-thymidylate synthase | ||
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-182 | DHFR | |||
Sequence similarities
In the N-terminal section; belongs to the dihydrofolate reductase family.
In the C-terminal section; belongs to the thymidylate synthase family.
Family and domain databases
Sequence
- Sequence statusFragment
- Length182
- Mass (Da)20,925
- Last updated1995-11-01 v1
- MD5 ChecksumBAFCE8C3F55819890DF2EACB2A45C650
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-terminal residue | 1 | ||||
Non-terminal residue | 182 | ||||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L28121 EMBL· GenBank· DDBJ | AAA29582.1 EMBL· GenBank· DDBJ | Genomic DNA |