P45996 · OMP53_HAEIF
- ProteinOuter membrane protein P5
- GeneompA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids359 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Acts as a fimbriae subunit, allowing adhesion to host cells.
With TolR probably plays a role in maintaining the position of the peptidoglycan cell wall in the periplasm. Acts as a porin with low permeability that allows slow penetration of small solutes; an internal gate slows down solute passage.
Miscellaneous
Non-typeable Haemophilus influenzae (NTHi) is a primary pathogen in otitis media (inflammation of the middle ear).
Biotechnology
Immunization of chinchillas with this protein provides partial protection against subsequent infection, suggesting it might be a useful vaccine candidate.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 86 | Part of salt bridge gating mechanism | ||||
Sequence: E | ||||||
Site | 181 | Part of salt bridge gating mechanism | ||||
Sequence: R |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell outer membrane | |
Cellular Component | pilus | |
Cellular Component | pore complex | |
Molecular Function | porin activity | |
Biological Process | cell adhesion | |
Biological Process | monoatomic ion transmembrane transport |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameOuter membrane protein P5
- Short namesOMP P5
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pasteurellales > Pasteurellaceae > Haemophilus
Accessions
- Primary accessionP45996
Subcellular Location
UniProt Annotation
GO Annotation
Cell outer membrane ; Multi-pass membrane protein
Note: Antisera against this protein label the whole length of fimbriae.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 27-37 | Beta stranded | ||||
Sequence: TFYAGVKAGQG | ||||||
Transmembrane | 64-75 | Beta stranded | ||||
Sequence: TFTYGVFGGYQI | ||||||
Transmembrane | 83-91 | Beta stranded | ||||
Sequence: LAAELGYDD | ||||||
Transmembrane | 110-121 | Beta stranded | ||||
Sequence: HGAYLSLKGSYE | ||||||
Transmembrane | 126-134 | Beta stranded | ||||
Sequence: LDVYGKAGV | ||||||
Transmembrane | 164-173 | Beta stranded | ||||
Sequence: GLFAVGAEYA | ||||||
Transmembrane | 178-185 | Beta stranded | ||||
Sequence: LAVRLEYQ | ||||||
Transmembrane | 211-219 | Beta stranded | ||||
Sequence: CINAGISYR |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No fimbrae seen on bacteria, the outer membrane blebs significantly, bacterial adhesion to human oropharyngeal cells decreases about 65%, significantly reduced ear infections in chinchilla.
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-21 | |||||
Sequence: MKKTAIALVVAGLAAASVAQA | ||||||
Chain | PRO_0000020109 | 22-359 | Outer membrane protein P5 | |||
Sequence: APQENTFYAGVKAGQGSFHDGINNNGAIKKGLSSSNYGYRRNTFTYGVFGGYQILNQDNFGLAAELGYDDFGRAKLREAGKPKAKHTNHGAYLSLKGSYEVLDGLDVYGKAGVALVRSDYKFYEDANGTRDHKKGRHTARASGLFAVGAEYAVLPELAVRLEYQWLTRVGKYRPQDKPNTAINYNPWIGCINAGISYRFGQGEAPVVAAPEMVSKTFSLNSDVTFAFGKANLKPQAQATLDSVYGEISQVKSRKVAVAGYTNRIGSDAFNVKLSQERADSVANYFVAKGVAADAISATGYGEANPVTGATCDQVKGRKALIACLAPDRRVEIAVNGTK | ||||||
Disulfide bond | 332↔344 | |||||
Sequence: CDQVKGRKALIAC |
Keywords
- PTM
Interaction
Subunit
Monomer and homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 233-359 | OmpA-like | ||||
Sequence: MVSKTFSLNSDVTFAFGKANLKPQAQATLDSVYGEISQVKSRKVAVAGYTNRIGSDAFNVKLSQERADSVANYFVAKGVAADAISATGYGEANPVTGATCDQVKGRKALIACLAPDRRVEIAVNGTK |
Domain
The extracellular loops are most variable in sequence, and in some bacteria confer sensitivity to phage and/or colicins.
Sequence similarities
Belongs to the outer membrane OOP (TC 1.B.6) superfamily. OmpA family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length359
- Mass (Da)38,340
- Last updated1995-11-01 v1
- Checksum576B1C59B4818C37
Keywords
- Technical term