P45858 · MMGD_BACSU
- ProteinCitrate/2-methylcitrate synthase
- GenemmgD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids372 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in both the tricarboxylic acid (TCA) and methylcitric acid cycles (PubMed:28956599).
Has both 2-methylcitrate synthase and citrate synthase activities. Catalyzes the condensation of propionyl-CoA and oxaloacetate to yield 2-methylcitrate (2-MC) and CoA, and the condensation of acetyl-CoA and oxaloacetate to yield citrate and CoA (PubMed:28956599, PubMed:8759838).
Has 2.3-fold higher activity as a 2-methylcitrate synthase (PubMed:28956599).
Catalyzes the formation of either (2S,3R)- or (2R,3S)-2-methylcitrate (PubMed:28956599).
Has both 2-methylcitrate synthase and citrate synthase activities. Catalyzes the condensation of propionyl-CoA and oxaloacetate to yield 2-methylcitrate (2-MC) and CoA, and the condensation of acetyl-CoA and oxaloacetate to yield citrate and CoA (PubMed:28956599, PubMed:8759838).
Has 2.3-fold higher activity as a 2-methylcitrate synthase (PubMed:28956599).
Catalyzes the formation of either (2S,3R)- or (2R,3S)-2-methylcitrate (PubMed:28956599).
Miscellaneous
Bifunctionality as a citrate and 2-methylcitrate synthase is likely important in the mother cell's physiological milieu.
Catalytic activity
- H2O + oxaloacetate + propanoyl-CoA = 2-methylcitrate + CoA + H+
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 188 | substrate | ||||
Sequence: H | ||||||
Active site | 223 | |||||
Sequence: H | ||||||
Binding site | 256-260 | CoA (UniProtKB | ChEBI) | ||||
Sequence: KIMGF | ||||||
Active site | 262 | |||||
Sequence: H | ||||||
Binding site | 272 | substrate | ||||
Sequence: R | ||||||
Active site | 314 | |||||
Sequence: D | ||||||
Binding site | 339 | substrate | ||||
Sequence: R | ||||||
Binding site | 358 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2-methylcitrate synthase activity | |
Molecular Function | citrate (Si)-synthase activity | |
Molecular Function | citrate synthase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | propionate metabolic process, methylcitrate cycle | |
Biological Process | sporulation resulting in formation of a cellular spore | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCitrate/2-methylcitrate synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionP45858
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000169930 | 1-372 | Citrate/2-methylcitrate synthase | |||
Sequence: MEEKQHYSPGLDGVIAAETHISYLDTQSSQILIRGYDLIELSETKSYLELVHLLLEGRLPEESEMETLERKINSASSLPADHLRLLELLPEDTHPMDGLRTGLSALAGYDRQIDDRSPSANKERAYQLLGKMPALTAASYRIINKKEPILPLQTLSYSANFLYMMTGKLPSSLEEQIFDRSLVLYSEHEMPNSTFAARVIASTHSDLYGALTGAVASLKGNLHGGANEAVMYLLLEAKTTSDFEQLLQTKLKRKEKIMGFGHRVYMKKMDPRALMMKEALQQLCDKAGDHRLYEMCEAGERLMEKEKGLYPNLDYYAAPVYWMLGIPIPLYTPIFFSARTSGLCAHVIEQHANNRLFRPRVSYMGPRYQTKS |
Proteomic databases
Expression
Induction
Subject to catabolite repression.
Developmental stage
Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)42,106
- Last updated1995-11-01 v1
- Checksum29FD292E27B5F9F7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U29084 EMBL· GenBank· DDBJ | AAB09616.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
D84432 EMBL· GenBank· DDBJ | BAA12590.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB14345.1 EMBL· GenBank· DDBJ | Genomic DNA |