P45818 · ROK1_YEAST

Function

function

ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity.

Catalytic activity

Features

Showing features for binding site.

156450100150200250300350400450500550
TypeIDPosition(s)Description
Binding site166-173ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Component90S preribosome
Cellular Componentnucleolus
Cellular Componentnucleoplasm
Cellular Componentsmall-subunit processome
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionATP-dependent activity, acting on RNA
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Biological Processendonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Biological Processendonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Biological Processendonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Biological Processmaturation of SSU-rRNA
Biological ProcesssnoRNA localization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent RNA helicase ROK1
  • EC number
  • Alternative names
    • Rescuer of KEM1 protein 1

Gene names

    • Name
      ROK1
    • ORF names
      G1651
    • Ordered locus names
      YGL171W

Organism names

Accessions

  • Primary accession
    P45818
  • Secondary accessions
    • D6VTY1

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis166No cell growth and 2-fold decrease in ATPase activity in vitro.
Mutagenesis172Slow cell growth.
Mutagenesis172No cell growth and drastic decrease in ATPase activity in vitro.
Mutagenesis280Slow cell growth.

Variants

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The viewer provides 17 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000550631-564ATP-dependent RNA helicase ROK1

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes. This association requires the presence of RRP5. Interacts also with OSH3.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P45818OSH3 P387133EBI-15686, EBI-12630
BINARY P45818UTP22 P532544EBI-15686, EBI-1878

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias, motif, domain.

TypeIDPosition(s)Description
Region1-45Disordered
Compositional bias31-45Basic and acidic residues
Compositional bias62-84Basic and acidic residues
Region62-87Disordered
Motif122-150Q motif
Domain153-333Helicase ATP-binding
Motif280-283DEAD box
Domain344-506Helicase C-terminal
Region512-564Disordered

Domain

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    564
  • Mass (Da)
    63,653
  • Last updated
    1995-11-01 v1
  • Checksum
    CACB950B8A15D5A2
MDIFRVLTRGASVKKESGPKAKAADYSVINGNDENHKEDNNESQIVKELDFFRNKRIISKVEDDREKTTENDSPNKEEKSGNDDGLIKPVITNTVEASALRKSYKGNVSGIDIPLPIGSFEDLISRFSFDKRLLNNLIENGFTEPTPIQCECIPVALNNRDVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSYKIFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKHLIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRVIIGHKEAANTNIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHELMYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVPGSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMAKMTRKEKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias31-45Basic and acidic residues
Compositional bias62-84Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z34901
EMBL· GenBank· DDBJ
CAA84384.1
EMBL· GenBank· DDBJ
Genomic DNA
X85757
EMBL· GenBank· DDBJ
CAA59758.1
EMBL· GenBank· DDBJ
Genomic DNA
Z72693
EMBL· GenBank· DDBJ
CAA96883.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA07942.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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