P45818 · ROK1_YEAST
- ProteinATP-dependent RNA helicase ROK1
- GeneROK1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids564 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | 90S preribosome | |
Cellular Component | nucleolus | |
Cellular Component | nucleoplasm | |
Cellular Component | small-subunit processome | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent activity, acting on RNA | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Biological Process | endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) | |
Biological Process | endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) | |
Biological Process | endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) | |
Biological Process | maturation of SSU-rRNA | |
Biological Process | snoRNA localization |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent RNA helicase ROK1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP45818
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 166 | No cell growth and 2-fold decrease in ATPase activity in vitro. | ||||
Sequence: G → D | ||||||
Mutagenesis | 172 | Slow cell growth. | ||||
Sequence: K → G, R, S, or W | ||||||
Mutagenesis | 172 | No cell growth and drastic decrease in ATPase activity in vitro. | ||||
Sequence: K → L | ||||||
Mutagenesis | 280 | Slow cell growth. | ||||
Sequence: D → A or V |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 17 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000055063 | 1-564 | ATP-dependent RNA helicase ROK1 | |||
Sequence: MDIFRVLTRGASVKKESGPKAKAADYSVINGNDENHKEDNNESQIVKELDFFRNKRIISKVEDDREKTTENDSPNKEEKSGNDDGLIKPVITNTVEASALRKSYKGNVSGIDIPLPIGSFEDLISRFSFDKRLLNNLIENGFTEPTPIQCECIPVALNNRDVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSYKIFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKHLIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRVIIGHKEAANTNIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHELMYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVPGSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMAKMTRKEKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK |
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes. This association requires the presence of RRP5. Interacts also with OSH3.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P45818 | OSH3 P38713 | 3 | EBI-15686, EBI-12630 | |
BINARY | P45818 | UTP22 P53254 | 4 | EBI-15686, EBI-1878 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-45 | Disordered | ||||
Sequence: MDIFRVLTRGASVKKESGPKAKAADYSVINGNDENHKEDNNESQI | ||||||
Compositional bias | 31-45 | Basic and acidic residues | ||||
Sequence: GNDENHKEDNNESQI | ||||||
Compositional bias | 62-84 | Basic and acidic residues | ||||
Sequence: EDDREKTTENDSPNKEEKSGNDD | ||||||
Region | 62-87 | Disordered | ||||
Sequence: EDDREKTTENDSPNKEEKSGNDDGLI | ||||||
Motif | 122-150 | Q motif | ||||
Sequence: DLISRFSFDKRLLNNLIENGFTEPTPIQC | ||||||
Domain | 153-333 | Helicase ATP-binding | ||||
Sequence: IPVALNNRDVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSYKIFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKHLIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRV | ||||||
Motif | 280-283 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 344-506 | Helicase C-terminal | ||||
Sequence: NIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHELMYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVPGSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMA | ||||||
Region | 512-564 | Disordered | ||||
Sequence: EKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK |
Domain
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities
Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length564
- Mass (Da)63,653
- Last updated1995-11-01 v1
- ChecksumCACB950B8A15D5A2
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 31-45 | Basic and acidic residues | ||||
Sequence: GNDENHKEDNNESQI | ||||||
Compositional bias | 62-84 | Basic and acidic residues | ||||
Sequence: EDDREKTTENDSPNKEEKSGNDD |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z34901 EMBL· GenBank· DDBJ | CAA84384.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X85757 EMBL· GenBank· DDBJ | CAA59758.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72693 EMBL· GenBank· DDBJ | CAA96883.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA07942.1 EMBL· GenBank· DDBJ | Genomic DNA |