P45688 · CSOSC_HALNC
- ProteinCarboxysome shell protein CsoS1C
- GenecsoS1C
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
One of shell proteins of the carboxysome, a polyhedral inclusion where RuBisCO (ribulose bisphosphate carboxylase, ccbL-ccbS) is sequestered. Assembles into hexamers which make sheets that form the facets of the polyhedral carboxysome (Probable). The shell probably limits the diffusion of CO2 into and out of the carboxysome (Probable). There are estimated to be 2970 CsoS1A/CsoS1C proteins per carboxysome (the proteins differ by only 1 residue) (Ref.4).
Unlike beta-carboxysomes, alpha-carboxysomes (Cb) can form without cargo protein. CsoS2 is essential for Cb formation and is also capable of targeting foreign proteins to the Cb. The Cb shell assembles with the aid of CsoS2; CsoS1A, CsoS1B and CsoS1C form the majority of the shell while CsoS4A and CsoS4B form vertices. CsoS1D forms pseudohexamers that probably control metabolite flux into and out of the shell.
Biotechnology
Expression of 10 genes for alpha-carboxysome (Cb) proteins (cbbL-cbbS-csoS2-csoS3-csoS4A-csoS4B-csoS1C-csoS1A-csoS1B-csoS1D) in E.coli generates compartments that resemble Cb, contain RuBisCO and have its catalytic activity, showing it is possible to make artificial, functional Cb using these 10 genes. Cargo proteins can be targeted to these organelles (PubMed:22184212, PubMed:30305640).
Artificial Cb assembly in E.coli requires csoS2-csoS4A-csoS4B-csoS1C-csoS1A-csoS1B-csoS1D (but not the gene for carbonic anhydrase, csoS3). Targeting proteins to the organelle requires at least one of the CsoS2 C-repeats; 3 repeats gives the best localization. A nanoreactor of the Cb shell proteins has been engineered which generates H2 using a ferredoxin-hydrogenase fusion (AC P07839-Q9FYU1) and a flavodoxin/ferredoxin--NADP reductase (AC A0A0K3QZA5) targeted separately to the Cb; the hydrogenase has first to be matured and activated by HydGXEF (AC Q8EAH9, Q8EAH8, Q8EAH7 and Q8EAH6 respectively). Encapsulation increases H2 production about 20% during anaerobic growth, and over 4-fold more during aerobic growth (PubMed:33116131).
Artificial Cb assembly in E.coli requires csoS2-csoS4A-csoS4B-csoS1C-csoS1A-csoS1B-csoS1D (but not the gene for carbonic anhydrase, csoS3). Targeting proteins to the organelle requires at least one of the CsoS2 C-repeats; 3 repeats gives the best localization. A nanoreactor of the Cb shell proteins has been engineered which generates H2 using a ferredoxin-hydrogenase fusion (AC P07839-Q9FYU1) and a flavodoxin/ferredoxin--NADP reductase (AC A0A0K3QZA5) targeted separately to the Cb; the hydrogenase has first to be matured and activated by HydGXEF (AC Q8EAH9, Q8EAH8, Q8EAH7 and Q8EAH6 respectively). Encapsulation increases H2 production about 20% during anaerobic growth, and over 4-fold more during aerobic growth (PubMed:33116131).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | carboxysome | |
Biological Process | carbon fixation |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameCarboxysome shell protein CsoS1C
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Chromatiales > Halothiobacillaceae > Halothiobacillus
Accessions
- Primary accessionP45688
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: This bacterium makes alpha-type carboxysomes.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Required for growth in ambient air.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000201514 | 1-98 | Carboxysome shell protein CsoS1C | |||
Sequence: MAAVTGIALGMIETRGLVPAIEAADAMTKAAEVRLVGRQFVGGGYVTVLVRGETGAVNAAVRAGADACERVGDGLVAAHIIARVHSEVENILPKAPEA |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-93 | BMC | ||||
Sequence: ALGMIETRGLVPAIEAADAMTKAAEVRLVGRQFVGGGYVTVLVRGETGAVNAAVRAGADACERVGDGLVAAHIIARVHSEVENILP |
Domain
The tight homohexamer forms a pore with an opening of about 2 Angstroms in diameter which is positively charged.
Sequence similarities
Belongs to the bacterial microcompartments protein family. CsoS1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length98
- Mass (Da)9,919
- Last updated1995-11-01 v1
- Checksum8107CCAF6DB19AC8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF038430 EMBL· GenBank· DDBJ | AAC32555.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001801 EMBL· GenBank· DDBJ | ACX95759.1 EMBL· GenBank· DDBJ | Genomic DNA |