P44868 · TRML_HAEIN
- ProteintRNA (cytidine(34)-2'-O)-methyltransferase
- GenetrmL
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids160 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.
Catalytic activity
- cytidine34 in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine34 in tRNA + S-adenosyl-L-homocysteine + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 100 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 122 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 130 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | identical protein binding | |
Molecular Function | RNA binding | |
Molecular Function | tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (cytidine(34)-2'-O)-methyltransferase activity | |
Biological Process | wobble position cytosine ribose methylation | |
Biological Process | wobble position uridine ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (cytidine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pasteurellales > Pasteurellaceae > Haemophilus
Accessions
- Primary accessionP44868
Proteomes
Subcellular Location
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000159819 | 1-160 | tRNA (cytidine34-2'-O)-methyltransferase | |||
Sequence: MLDIVLYEPEIPQNTGNIIRLCANTGFRLHLIEPLGFTWDDKRLRRSGLDYHEFAEIKRHKTFEAFLESEKPKRLFALTTKGCPAHSQVKFKLGDYLMFGPETRGIPMSILNEMPMEQKIRIPMTANSRSMNLSNSVAVTVYEAWRQLGYKGAVNLPEVK |
Interaction
Subunit
Homodimer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P44868 | trmL P44868 | 4 | EBI-15618808, EBI-15618808 |
Protein-protein interaction databases
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length160
- Mass (Da)18,401
- Last updated1995-11-01 v1
- Checksum0E09ADB31E575026
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L42023 EMBL· GenBank· DDBJ | AAC22424.1 EMBL· GenBank· DDBJ | Genomic DNA |