P43605 · ECO1_YEAST
- ProteinN-acetyltransferase ECO1
- GeneECO1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids281 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for establishment of sister chromatid cohesion during S phase but not for its further maintenance during G2 or M phases or for loading the cohesin complex onto DNA. Interacts with the three known alternate replication factor C (RFC) complexes, suggesting that these complexes have essential but redundant activity in cohesion establishment. Acts by acetylating the cohesin complex component SMC3. In vitro, possesses acetyltransferase activity where it can acetylate itself and components of the cohesin complex (MCD1, IRR1 and PDS5), but is unable to acetylate histones.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromatin | |
Cellular Component | cytoplasm | |
Cellular Component | nuclear replication fork | |
Cellular Component | nucleus | |
Molecular Function | acetyltransferase activity | |
Molecular Function | chromatin binding | |
Molecular Function | metal ion binding | |
Molecular Function | peptide-lysine-N-acetyltransferase activity | |
Biological Process | chromatin looping | |
Biological Process | chromosome organization | |
Biological Process | DNA repair | |
Biological Process | DNA replication | |
Biological Process | double-strand break repair | |
Biological Process | establishment of meiotic sister chromatid cohesion | |
Biological Process | establishment of mitotic sister chromatid cohesion | |
Biological Process | mitotic chromosome condensation | |
Biological Process | mitotic sister chromatid cohesion | |
Biological Process | regulation of mitotic nuclear division | |
Biological Process | telomere organization | |
Biological Process | tRNA gene clustering |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetyltransferase ECO1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP43605
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 35 | In ctf7-109; loss of function. | ||||
Sequence: C → Y | ||||||
Mutagenesis | 53 | In ctf7-108; loss of function. | ||||
Sequence: H → Y | ||||||
Mutagenesis | 211 | Abolishes acetyltransferase activity; but not chromatid cohesion activity. | ||||
Sequence: G → D | ||||||
Mutagenesis | 222-223 | Abolishes acetyltransferase activity; but not chromatid cohesion activity. | ||||
Sequence: RK → GG | ||||||
Mutagenesis | 225 | Abolishes acetyltransferase activity; but not chromatid cohesion activity. | ||||
Sequence: G → D | ||||||
Mutagenesis | 232 | Abolishes acetyltransferase activity; but not chromatid cohesion activity. | ||||
Sequence: D → G |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 5 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000074550 | 1-281 | N-acetyltransferase ECO1 | |||
Sequence: MKARKSQRKAGSKPNLIQSKLQVNNGSKSNKIVKCDKCEMSYSSTSIEDRAIHEKYHTLQLHGRKWSPNWGSIVYTERNHSRTVHLSRSTGTITPLNSSPLKKSSPSITHQEEKIVYVRPDKSNGEVRAMTEIMTLVNNELNAPHDENVIWNSTTEEKGKAFVYIRNDRAVGIIIIENLYGGNGKTSSRGRWMVYDSRRLVQNVYPDFKIGISRIWVCRTARKLGIATKLIDVARENIVYGEVIPRYQVAWSQPTDSGGKLASKYNGIMHKSGKLLLPVYI | ||||||
Modified residue | 223 | N6-acetyllysine; by autocatalysis | ||||
Sequence: K |
Post-translational modification
Autoacetylates in vitro.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Binds specifically to CHL12, RFC1, RFC2, RFC3, RFC4, RFC5 and RAD24 when members of an RFC complex. Interacts with CHL1 and MPS3.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P43605 | CHL1 P22516 | 2 | EBI-22988, EBI-4600 | |
BINARY | P43605 | CTF18 P49956 | 2 | EBI-22988, EBI-4560 | |
BINARY | P43605 | MPS3 P47069 | 5 | EBI-22988, EBI-25811 | |
BINARY | P43605 | POL30 P15873 | 2 | EBI-22988, EBI-12993 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for zinc finger, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 33-57 | CCHH-type | ||||
Sequence: VKCDKCEMSYSSTSIEDRAIHEKYH | ||||||
Region | 86-105 | Disordered | ||||
Sequence: LSRSTGTITPLNSSPLKKSS |
Sequence similarities
Belongs to the acetyltransferase family. ECO subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length281
- Mass (Da)31,845
- Last updated1995-11-01 v1
- Checksum0AF1B59BE9E71818
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D50617 EMBL· GenBank· DDBJ | BAA09266.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006940 EMBL· GenBank· DDBJ | DAA12467.1 EMBL· GenBank· DDBJ | Genomic DNA |