P43508 · CPR4_CAEEL

  • Protein
    Cathepsin B-like cysteine proteinase 4
  • Gene
    cpr-4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Thiol protease which shows activity against the fluorogenic substrate z-Arg-Arg-AMC.

Miscellaneous

Mediates the radiation-induced bystander effect (RIBE), a process in which factors released by irradiated cells or tissues exert effects on unexposed cells or tissues. Following localized ultraviolet irradiation of the head, mediates RIBE in multiple unexposed regions including the posterior region. Also leads to RIBE including inhibition of cell death and increased embryonic lethality of progeny in unirradiated animals exposed to the culture medium of irradiated animals. Likely to exert RIBE by acting through the insulin-like receptor daf-2.

Features

Showing features for active site.

133550100150200250300
TypeIDPosition(s)Description
Active site109
Active site281
Active site301

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componentextracellular space
Molecular Functioncysteine-type endopeptidase activity
Biological Processcellular response to gamma radiation
Biological Processcellular response to UV
Biological Processproteolysis involved in protein catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Cathepsin B-like cysteine proteinase 4
  • EC number
  • Alternative names
    • Cysteine protease-related 4

Gene names

    • Name
      cpr-4
    • ORF names
      F44C4.3

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    P43508

Proteomes

Organism-specific databases

Subcellular Location

Secreted
Note: Secreted from animals irradiated with ultraviolet or ionizing gamma rays into the culture medium.

Keywords

Phenotypes & Variants

Disruption phenotype

RNAi-mediated knockdown results in greatly reduced radiation-induced bystander effect (RIBE) activity in the culture medium of irradiated animals.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis109Loss of protease and RIBE activity.
Mutagenesis281Loss of protease and RIBE activity.
Mutagenesis301No effect on protease or RIBE activity.

PTM/Processing

Features

Showing features for signal, propeptide, chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
Signal1-15
PropeptidePRO_000002619416-80
ChainPRO_000002619581-335Cathepsin B-like cysteine proteinase 4
Disulfide bond94↔123
Disulfide bond106↔150
Disulfide bond142↔209
Disulfide bond143↔146
Disulfide bond179↔213
Disulfide bond187↔199
Glycosylation193N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Induction

Induced by ultraviolet and ionizing radiation through a cep-1-dependent mechanism.

Developmental stage

Not detected in the embryo, observed in the intestine of early stage L1-L3 larvae, peaks at the L4 larval stage and declines in adulthood.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the peptidase C1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    335
  • Mass (Da)
    36,493
  • Last updated
    1995-11-01 v1
  • Checksum
    285900FAB876CED0
MKYLILAALVAVTAGLVIPLVPKTQEAITEYVNSKQSLWKAEIPKDITIEQVKKRLMRTEFVAPHTPDVEVVKHDINEDTIPATFDARTQWPNCMSINNIRDQSDCGSCWAFAAAEAASDRFCIASNGAVNTLLSAEDVLSCCSNCGYGCEGGYPINAWKYLVKSGFCTGGSYEAQFGCKPYSLAPCGETVGNVTWPSCPDDGYDTPACVNKCTNKNYNVAYTADKHFGSTAYAVGKKVSQIQAEIIAHGPVEAAFTVYEDFYQYKTGVYVHTTGQELGGHAIRILGWGTDNGTPYWLVANSWNVNWGENGYFRIIRGTNECGIEHAVVGGVPKV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L39895
EMBL· GenBank· DDBJ
AAA98785.1
EMBL· GenBank· DDBJ
mRNA
L39926
EMBL· GenBank· DDBJ
AAA98783.1
EMBL· GenBank· DDBJ
Genomic DNA
FO081381
EMBL· GenBank· DDBJ
CCD71204.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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