P43508 · CPR4_CAEEL
- ProteinCathepsin B-like cysteine proteinase 4
- Genecpr-4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids335 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Thiol protease which shows activity against the fluorogenic substrate z-Arg-Arg-AMC.
Miscellaneous
Mediates the radiation-induced bystander effect (RIBE), a process in which factors released by irradiated cells or tissues exert effects on unexposed cells or tissues. Following localized ultraviolet irradiation of the head, mediates RIBE in multiple unexposed regions including the posterior region. Also leads to RIBE including inhibition of cell death and increased embryonic lethality of progeny in unirradiated animals exposed to the culture medium of irradiated animals. Likely to exert RIBE by acting through the insulin-like receptor daf-2.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 109 | |||||
Sequence: C | ||||||
Active site | 281 | |||||
Sequence: H | ||||||
Active site | 301 | |||||
Sequence: N |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | extracellular space | |
Molecular Function | cysteine-type endopeptidase activity | |
Biological Process | cellular response to gamma radiation | |
Biological Process | cellular response to UV | |
Biological Process | proteolysis involved in protein catabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCathepsin B-like cysteine proteinase 4
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionP43508
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Secreted from animals irradiated with ultraviolet or ionizing gamma rays into the culture medium.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown results in greatly reduced radiation-induced bystander effect (RIBE) activity in the culture medium of irradiated animals.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 109 | Loss of protease and RIBE activity. | ||||
Sequence: C → A | ||||||
Mutagenesis | 281 | Loss of protease and RIBE activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 301 | No effect on protease or RIBE activity. | ||||
Sequence: N → A |
PTM/Processing
Features
Showing features for signal, propeptide, chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-15 | |||||
Sequence: MKYLILAALVAVTAG | ||||||
Propeptide | PRO_0000026194 | 16-80 | ||||
Sequence: LVIPLVPKTQEAITEYVNSKQSLWKAEIPKDITIEQVKKRLMRTEFVAPHTPDVEVVKHDINEDT | ||||||
Chain | PRO_0000026195 | 81-335 | Cathepsin B-like cysteine proteinase 4 | |||
Sequence: IPATFDARTQWPNCMSINNIRDQSDCGSCWAFAAAEAASDRFCIASNGAVNTLLSAEDVLSCCSNCGYGCEGGYPINAWKYLVKSGFCTGGSYEAQFGCKPYSLAPCGETVGNVTWPSCPDDGYDTPACVNKCTNKNYNVAYTADKHFGSTAYAVGKKVSQIQAEIIAHGPVEAAFTVYEDFYQYKTGVYVHTTGQELGGHAIRILGWGTDNGTPYWLVANSWNVNWGENGYFRIIRGTNECGIEHAVVGGVPKV | ||||||
Disulfide bond | 94↔123 | |||||
Sequence: CMSINNIRDQSDCGSCWAFAAAEAASDRFC | ||||||
Disulfide bond | 106↔150 | |||||
Sequence: CGSCWAFAAAEAASDRFCIASNGAVNTLLSAEDVLSCCSNCGYGC | ||||||
Disulfide bond | 142↔209 | |||||
Sequence: CCSNCGYGCEGGYPINAWKYLVKSGFCTGGSYEAQFGCKPYSLAPCGETVGNVTWPSCPDDGYDTPAC | ||||||
Disulfide bond | 143↔146 | |||||
Sequence: CSNC | ||||||
Disulfide bond | 179↔213 | |||||
Sequence: CKPYSLAPCGETVGNVTWPSCPDDGYDTPACVNKC | ||||||
Disulfide bond | 187↔199 | |||||
Sequence: CGETVGNVTWPSC | ||||||
Glycosylation | 193 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
Induced by ultraviolet and ionizing radiation through a cep-1-dependent mechanism.
Developmental stage
Not detected in the embryo, observed in the intestine of early stage L1-L3 larvae, peaks at the L4 larval stage and declines in adulthood.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length335
- Mass (Da)36,493
- Last updated1995-11-01 v1
- Checksum285900FAB876CED0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L39895 EMBL· GenBank· DDBJ | AAA98785.1 EMBL· GenBank· DDBJ | mRNA | ||
L39926 EMBL· GenBank· DDBJ | AAA98783.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FO081381 EMBL· GenBank· DDBJ | CCD71204.1 EMBL· GenBank· DDBJ | Genomic DNA |