P43150 · GP63_LEIME

  • Protein
    Leishmanolysin C1
  • Gene
    gp63-C1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    4/5

Function

function

Has an integral role during the infection of macrophages in the mammalian host.

Catalytic activity

  • Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-|-Leu-Lys-Lys-.
    EC:3.4.24.36 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site, active site.

164650100150200250300350400450500550600
TypeIDPosition(s)Description
Binding site266Zn2+ (UniProtKB | ChEBI); catalytic
Active site267
Binding site270Zn2+ (UniProtKB | ChEBI); catalytic
Binding site336Zn2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionmetal ion binding
Molecular Functionmetalloendopeptidase activity
Biological Processcell adhesion
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Leishmanolysin C1
  • EC number
  • Alternative names
    • Cell surface protease
    • Major surface glycoprotein
    • Major surface protease
    • Promastigote surface endopeptidase
    • Protein gp63

Gene names

    • Name
      gp63-C1

Organism names

  • Taxonomic identifier
  • Strain
    • MNYC/BZ/62/M379
  • Taxonomic lineage
    Eukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Leishmaniinae > Leishmania

Accessions

  • Primary accession
    P43150

Organism-specific databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain42-606Extracellular
Transmembrane607-627Helical
Topological domain628-646Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for signal, propeptide, glycosylation, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-39
PropeptidePRO_000002867040-102Activation peptide
Glycosylation86N-linked (GlcNAc...) asparagine
ChainPRO_0000028671103-646Leishmanolysin C1
Disulfide bond127↔144
Disulfide bond193↔232
Glycosylation297N-linked (GlcNAc...) asparagine
Disulfide bond316↔388
Disulfide bond395↔458
Glycosylation399N-linked (GlcNAc...) asparagine
Disulfide bond408↔427
Glycosylation409N-linked (GlcNAc...) asparagine
Disulfide bond417↔492
Glycosylation433N-linked (GlcNAc...) asparagine
Glycosylation445N-linked (GlcNAc...) asparagine
Glycosylation466N-linked (GlcNAc...) asparagine
Disulfide bond469↔513
Glycosylation501N-linked (GlcNAc...) asparagine
Disulfide bond518↔568
Disulfide bond538↔561

Keywords

PTM databases

Expression

Developmental stage

Expressed in both the promastigote and the amastigote forms.

Structure

Family & Domains

Sequence similarities

Belongs to the peptidase M8 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    646
  • Mass (Da)
    69,054
  • Last updated
    1995-11-01 v1
  • Checksum
    FE448DDC78C10B0A
MPVDSSSTHRHRCVAAPLVRLAAAGAAVTVAVGTAAAWAHAGAPQHRCIHDAMQARVLQSVAAQRMAPSAVSAVGLPYVSVVPVENASTLDYSLSDSTSPGVVRAANWGALRVAVSAEDLTDPAYHCARVGQQVNNHAGDIVTCTAEDILTDEKRDTLVKHLVPQALQLHRERLKVRQVQGKWKVTGMADVICGDFKVPPEHITEGVTNTDFVLYVASVPSEESVLAWATTCQVFPDGHPAVGVINIPAANIASRYDQLVTRVVTHEMAHAVGFSGTFFGAVGIVQEVPHLRRKDFNVSVITSSTVVAKAREQYGCNSLEYLEIEDQGGAGSAGSHIKMRNAKDELMAPAASAGYYTALTMAVFQDLGFYQADFSKAEEMPWGRNVGCAFLSEKCMAKNVTKWPAMFCNESAATIRCPTDRLRVGTCGITAYNTSLATYWQYFTNASLGGYSPFLDYCPFVVGYRNGSCNQDASTTPDLLAAFNVFSEAARCIDGAFTPKNRTAADGYYTALCANVKCDTATRTYSVQVRGTNGYANCTPGLRVKLSSVSDAFEKGGYVTCPPYVEVCQGNVKAAKDFAGDTDSSSSADDAADKEAMQRWSDRMAALATATTLLLGMVLSLMALLVVRLLLTSSPWCCCRLGGLPT

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X64394
EMBL· GenBank· DDBJ
CAA45733.1
EMBL· GenBank· DDBJ
mRNA

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