P43027 · GDF5_MOUSE
- ProteinGrowth/differentiation factor 5
- GeneGdf5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids495 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (By similarity).
Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (By similarity).
Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (PubMed:24076600).
Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (By similarity).
Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (By similarity).
Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (PubMed:24076600).
Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGrowth/differentiation factor 5
- Short namesGDF-5
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP43027
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Involvement in disease
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 98 | |||||
Sequence: P → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 22 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, propeptide, glycosylation, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-27 | |||||
Sequence: MRLPKLLTLLLWHLAWLDLELICTVLG | ||||||
Propeptide | PRO_0000033914 | 28-375 | ||||
Sequence: APDLGQRTPGAKPGLTKAEAKERPPLARNVFRPGGHIYGVGATNARAKGSSGQTQAKKDEPRKMPPRSGGPETKPGPSSQTRQAAARTVTPKGQLPGGKASSKAGSAPSSFLLKKTREPGTPREPKEPFRPPPITPHEYMLSLYRTLSDADRKGGNSSVKLEAGLANTITSFIDKGQDDRGPAVRKQRYVFDISALEKDGLLGAELRILRKKPLDVAKPAVPSSGRVAQLKLSSCPSGRQPAALLDVRSVPGLDGSGWEVFDIWKLFRNFKNSAQLCLELEAWERGRAVDLRGLGFERTARQVHEKALFLVFGRTKKRDLFFNEIKARSGQDDKTVYEYLFSQRRKRR | ||||||
Glycosylation | 183 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Chain | PRO_0000033915 | 376-495 | Growth/differentiation factor 5 | |||
Sequence: APLANRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR | ||||||
Disulfide bond | 394↔460 | |||||
Sequence: CSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCC | ||||||
Disulfide bond | 423↔492 | |||||
Sequence: CEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESC | ||||||
Disulfide bond | 427↔494 | |||||
Sequence: CEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGC | ||||||
Disulfide bond | 459 | Interchain | ||||
Sequence: C |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
Induced over 300-fold in tibialis anterior muscles 3 days following denervation. High expression is maintained at least until 10 days after denervation.
Gene expression databases
Interaction
Subunit
Homodimer; disulfide-linked (By similarity).
Interacts with serine proteases, HTRA1 and HTRA3 (PubMed:14973287, PubMed:15206957).
Following LPS binding, may form a complex with CXCR4, HSP90AA1 and HSPA8 (By similarity).
Interacts with high affinity with NOG; inhibits chondrogenesis (By similarity).
Interacts with high affinity with BMPR1B and lower affinity with BMPR1A; positively regulates chondrocyte differentiation and induces SMAD-dependent signaling. Interacts with FBN1 (via N-terminal domain) and FBN2 (By similarity).
Interacts with serine proteases, HTRA1 and HTRA3 (PubMed:14973287, PubMed:15206957).
Following LPS binding, may form a complex with CXCR4, HSP90AA1 and HSPA8 (By similarity).
Interacts with high affinity with NOG; inhibits chondrogenesis (By similarity).
Interacts with high affinity with BMPR1B and lower affinity with BMPR1A; positively regulates chondrocyte differentiation and induces SMAD-dependent signaling. Interacts with FBN1 (via N-terminal domain) and FBN2 (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 30-162 | Disordered | ||||
Sequence: DLGQRTPGAKPGLTKAEAKERPPLARNVFRPGGHIYGVGATNARAKGSSGQTQAKKDEPRKMPPRSGGPETKPGPSSQTRQAAARTVTPKGQLPGGKASSKAGSAPSSFLLKKTREPGTPREPKEPFRPPPIT | ||||||
Compositional bias | 80-94 | Basic and acidic residues | ||||
Sequence: QTQAKKDEPRKMPPR | ||||||
Compositional bias | 100-115 | Polar residues | ||||
Sequence: TKPGPSSQTRQAAART |
Sequence similarities
Belongs to the TGF-beta family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length495
- Mass (Da)54,895
- Last updated2011-07-27 v2
- Checksum8FAECD5A9C996D6E
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 80-94 | Basic and acidic residues | ||||
Sequence: QTQAKKDEPRKMPPR | ||||||
Compositional bias | 100-115 | Polar residues | ||||
Sequence: TKPGPSSQTRQAAART |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U08337 EMBL· GenBank· DDBJ | AAA18778.1 EMBL· GenBank· DDBJ | mRNA | ||
AL845445 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466551 EMBL· GenBank· DDBJ | EDL06156.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC034546 EMBL· GenBank· DDBJ | AAH34546.1 EMBL· GenBank· DDBJ | mRNA |