P42325 · NCAH_DROME
- ProteinNeurocalcin homolog
- GeneNca
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids190 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Calcium-binding protein that is essential for promoting night-time sleep and inhibiting nocturnal hyperactivity (PubMed:30865587, PubMed:7989365).
During the night-time it promotes sleep by limiting synaptic output from arousal- and wake-promoting neurons within the C01- and A05- domains that include the mushroom body, and the antennal mechanosensory and motor center (PubMed:30865587).
Regulated by the circadian clock network and light-sensing circuits that include the CRY photoreceptor; it is likely that both of these internal and external cues are required to define the temporal window (the night-time) during which this protein functions (PubMed:30865587).
Inhibits the phosphorylation of rhodopsin in a calcium-dependent manner (PubMed:8626592).
During the night-time it promotes sleep by limiting synaptic output from arousal- and wake-promoting neurons within the C01- and A05- domains that include the mushroom body, and the antennal mechanosensory and motor center (PubMed:30865587).
Regulated by the circadian clock network and light-sensing circuits that include the CRY photoreceptor; it is likely that both of these internal and external cues are required to define the temporal window (the night-time) during which this protein functions (PubMed:30865587).
Inhibits the phosphorylation of rhodopsin in a calcium-dependent manner (PubMed:8626592).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 73 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 75 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 77 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 79 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 84 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 109 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 111 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 113 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 115 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 120 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 157 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 159 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 161 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 163 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 168 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Molecular Function | calcium ion binding | |
Biological Process | negative regulation of neuronal signal transduction | |
Biological Process | positive regulation of circadian sleep/wake cycle, sleep |
Keywords
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNeurocalcin homolog
- Short namesDrosNCa
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionP42325
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Viable. Adults exhibit reduced night-time sleep but daytime sleep appears normal. RNAi-mediated knockdown in the whole organism or simultaneously knockdown in the C01 and A05 neurons, results in a similar phenotype to the genetic knockout. As demonstrated in adults undergoing RNAi-mediated knockdown in the C01 and A05 neurons, the reduction in night-time sleep and increase in night-time arousal is likely the result of enhanced neurotransmitter release in the mushroom body alpha-beta lobes, and the antennal mechanosensory and motor center, which occurs only during the dark period of different light-dark conditions. RNAi-mediated knockdown in a tim mutant background, also results in sleep loss during the dark period of different light-dark conditions but under constant light conditions sleep loss is completely suppressed. RNAi-mediated knockdown in a cry mutant background, results in a small but significant reduction in sleep under LL conditions.
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine | ||||
Sequence: G | ||||||
Chain | PRO_0000073801 | 2-190 | Neurocalcin homolog | |||
Sequence: GKQNSKLKPEVLEDLKQNTEFTDAEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFLCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTAIYKMVGSVMKMPEDESTPEKRTDKIFRQMDRNKDGKLSLEEFIEGAKSDPSIVRLLQCDPQSH |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in neuronal tissues. High level expression seen in the cortical regions of the central brain and lower levels in the lamina, the first optic lobe of the brain. It is also found in the thoracic ganglia.
Developmental stage
Found in the embryos, larvae and pupae. Expression in the adult heads is higher than in the bodies.
Gene expression databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P42325 | CG15149 Q4V3Y0 | 4 | EBI-149848, EBI-15122292 | |
BINARY | P42325 | Dmel\CG13510 Q9W221 | 4 | EBI-149848, EBI-130463 | |
BINARY | P42325 | Obp19d P54192 | 4 | EBI-149848, EBI-26770067 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 40-58 | EF-hand 1 | ||||
Sequence: SGHLSVEEFKKIYGNFFPY | ||||||
Domain | 60-95 | EF-hand 2 | ||||
Sequence: DASKFAEHVFRTFDANGDGTIDFREFLCALSVTSRG | ||||||
Domain | 96-131 | EF-hand 3 | ||||
Sequence: KLEQKLKWAFSMYDLDGNGYISRQEMLEIVTAIYKM | ||||||
Domain | 144-179 | EF-hand 4 | ||||
Sequence: TPEKRTDKIFRQMDRNKDGKLSLEEFIEGAKSDPSI |
Sequence similarities
Belongs to the recoverin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length190
- Mass (Da)21,894
- Last updated2007-01-23 v2
- ChecksumF1158FEB8CF13F64
Mass Spectrometry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U15735 EMBL· GenBank· DDBJ | AAA62152.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014296 EMBL· GenBank· DDBJ | AAF49082.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY071612 EMBL· GenBank· DDBJ | AAL49234.1 EMBL· GenBank· DDBJ | mRNA |