P42325 · NCAH_DROME

Function

function

Calcium-binding protein that is essential for promoting night-time sleep and inhibiting nocturnal hyperactivity (PubMed:30865587, PubMed:7989365).
During the night-time it promotes sleep by limiting synaptic output from arousal- and wake-promoting neurons within the C01- and A05- domains that include the mushroom body, and the antennal mechanosensory and motor center (PubMed:30865587).
Regulated by the circadian clock network and light-sensing circuits that include the CRY photoreceptor; it is likely that both of these internal and external cues are required to define the temporal window (the night-time) during which this protein functions (PubMed:30865587).
Inhibits the phosphorylation of rhodopsin in a calcium-dependent manner (PubMed:8626592).

Features

Showing features for binding site.

119020406080100120140160180
TypeIDPosition(s)Description
Binding site73Ca2+ 1 (UniProtKB | ChEBI)
Binding site75Ca2+ 1 (UniProtKB | ChEBI)
Binding site77Ca2+ 1 (UniProtKB | ChEBI)
Binding site79Ca2+ 1 (UniProtKB | ChEBI)
Binding site84Ca2+ 1 (UniProtKB | ChEBI)
Binding site109Ca2+ 2 (UniProtKB | ChEBI)
Binding site111Ca2+ 2 (UniProtKB | ChEBI)
Binding site113Ca2+ 2 (UniProtKB | ChEBI)
Binding site115Ca2+ 2 (UniProtKB | ChEBI)
Binding site120Ca2+ 2 (UniProtKB | ChEBI)
Binding site157Ca2+ 3 (UniProtKB | ChEBI)
Binding site159Ca2+ 3 (UniProtKB | ChEBI)
Binding site161Ca2+ 3 (UniProtKB | ChEBI)
Binding site163Ca2+ 3 (UniProtKB | ChEBI)
Binding site168Ca2+ 3 (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Functioncalcium ion binding
Biological Processnegative regulation of neuronal signal transduction
Biological Processpositive regulation of circadian sleep/wake cycle, sleep

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Neurocalcin homolog
  • Short names
    DrosNCa

Gene names

    • Name
      Nca
    • ORF names
      CG7641

Organism names

  • Taxonomic identifier
  • Strains
    • Canton-S
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    P42325
  • Secondary accessions
    • Q9VW67

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Viable. Adults exhibit reduced night-time sleep but daytime sleep appears normal. RNAi-mediated knockdown in the whole organism or simultaneously knockdown in the C01 and A05 neurons, results in a similar phenotype to the genetic knockout. As demonstrated in adults undergoing RNAi-mediated knockdown in the C01 and A05 neurons, the reduction in night-time sleep and increase in night-time arousal is likely the result of enhanced neurotransmitter release in the mushroom body alpha-beta lobes, and the antennal mechanosensory and motor center, which occurs only during the dark period of different light-dark conditions. RNAi-mediated knockdown in a tim mutant background, also results in sleep loss during the dark period of different light-dark conditions but under constant light conditions sleep loss is completely suppressed. RNAi-mediated knockdown in a cry mutant background, results in a small but significant reduction in sleep under LL conditions.

PTM/Processing

Features

Showing features for initiator methionine, lipidation, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Lipidation2N-myristoyl glycine
ChainPRO_00000738012-190Neurocalcin homolog

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in neuronal tissues. High level expression seen in the cortical regions of the central brain and lower levels in the lamina, the first optic lobe of the brain. It is also found in the thoracic ganglia.

Developmental stage

Found in the embryos, larvae and pupae. Expression in the adult heads is higher than in the bodies.

Gene expression databases

    • FBgn0013303Expressed in head capsule and 25 other cell types or tissues

Interaction

Binary interactions

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain40-58EF-hand 1
Domain60-95EF-hand 2
Domain96-131EF-hand 3
Domain144-179EF-hand 4

Sequence similarities

Belongs to the recoverin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    190
  • Mass (Da)
    21,894
  • Last updated
    2007-01-23 v2
  • Checksum
    F1158FEB8CF13F64
MGKQNSKLKPEVLEDLKQNTEFTDAEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFLCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTAIYKMVGSVMKMPEDESTPEKRTDKIFRQMDRNKDGKLSLEEFIEGAKSDPSIVRLLQCDPQSH

Mass Spectrometry

Molecular mass is 21,975.51 Da. Determined by Electrospray.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U15735
EMBL· GenBank· DDBJ
AAA62152.1
EMBL· GenBank· DDBJ
mRNA
AE014296
EMBL· GenBank· DDBJ
AAF49082.1
EMBL· GenBank· DDBJ
Genomic DNA
AY071612
EMBL· GenBank· DDBJ
AAL49234.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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