P41830 · CNR14_CAEEL
- ProteinSteroid hormone receptor family member cnr14
- Genesex-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids534 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Transcriptional regulator which is involved in the sex determination and X chromosome dosage compensation pathways (PubMed:16139225, PubMed:17720939, PubMed:21471153, PubMed:23666922, PubMed:33372658).
Directly binds to five 5'-A(G/C)(G/T)(T/G)C(A/G)-3' sites in the promoter of sex-determining factor xol-1 to negatively regulate its expression and promote hermaphrodite development (PubMed:23666922).
Together with fox-1 is involved in making the distinction between one and two X-chromosomes (PubMed:21471153, PubMed:23666922, PubMed:33372658).
Plays a role in the fox-1-mediated repression of the functionally active isoform (isoform b) of the sex-determining factor xol-1 gene to promote hermaphrodite development (PubMed:33372658).
Plays a role in the association of the dosage compensation complex proteins dpy-27 and sdc-3 with the hermaphrodite X chromosomes (PubMed:16139225, PubMed:17720939).
Directly binds to five 5'-A(G/C)(G/T)(T/G)C(A/G)-3' sites in the promoter of sex-determining factor xol-1 to negatively regulate its expression and promote hermaphrodite development (PubMed:23666922).
Together with fox-1 is involved in making the distinction between one and two X-chromosomes (PubMed:21471153, PubMed:23666922, PubMed:33372658).
Plays a role in the fox-1-mediated repression of the functionally active isoform (isoform b) of the sex-determining factor xol-1 gene to promote hermaphrodite development (PubMed:33372658).
Plays a role in the association of the dosage compensation complex proteins dpy-27 and sdc-3 with the hermaphrodite X chromosomes (PubMed:16139225, PubMed:17720939).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 148-223 | Nuclear receptor | ||||
Sequence: ISFCKVCGDKASGYHYGVTSCEGCKGFFRRSIQRKIDYRCLKQQVCEIKRESRNRCQYCRFKKCLDSGMSKDSVRQ |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | nuclear receptor activity | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Molecular Function | RNA polymerase II transcription regulatory region sequence-specific DNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | cell differentiation | |
Biological Process | dosage compensation by hypoactivation of X chromosome | |
Biological Process | hormone-mediated signaling pathway | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | primary sex determination | |
Biological Process | regulation of alternative mRNA splicing, via spliceosome | |
Biological Process | sex differentiation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSteroid hormone receptor family member cnr14
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionP41830
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown results in 43% lethality of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown together with fox-1 results in hermaphrodite embryonic lethality (PubMed:21471153, PubMed:33372658).
This hermaphrodite-specific lethality is suppressed in a sea-2 bp283 mutant or sea-1 gk799 mutant background (PubMed:21471153).
RNAi-mediated knockdown results in hermaphrodites lethality due to failure of the dosage compensation complex to assemble on X chromosomes in a fox-1 y303 mutant background (PubMed:17720939).
RNAi-mediated knockdown in a background containing one copy of the fox-1 gene results in the viability of 3% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of the xol-1 gene have been mutated to AUACA and AUAUA, respectively results in the viability of 1% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of one copy of the xol-1 gene have been mutated to AUACA and AUAUA, respectively results in the viability of 7% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG motifs in intron 6 of the xol-1 gene have been mutated to AUACA results in the viability of 24% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG motifs in intron 6 of one copy of the xol-1 gene have been mutated to AUACA results in the viability of 18% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCUAG motifs in intron 6 of the xol-1 gene have been mutated to AUAUA results in the viability of 33% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCUAG motifs in intron 6 of one copy of the xol-1 gene have been mutated to AUAUA results in the viability of 13% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of the xol-1 gene have been mutated to GCUUG results in the viability of 8% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of one copy of the xol-1 gene have been mutated to GCUUG results in the viability of 36% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in strains containing five or three fox-1-binding GCACG motifs in intron 6 of the xol-1 gene results in the viability of 49% and 33% of hermaphrodites, respectively (PubMed:33372658).
RNAi-mediated knockdown together with fox-1 results in hermaphrodite embryonic lethality (PubMed:21471153, PubMed:33372658).
This hermaphrodite-specific lethality is suppressed in a sea-2 bp283 mutant or sea-1 gk799 mutant background (PubMed:21471153).
RNAi-mediated knockdown results in hermaphrodites lethality due to failure of the dosage compensation complex to assemble on X chromosomes in a fox-1 y303 mutant background (PubMed:17720939).
RNAi-mediated knockdown in a background containing one copy of the fox-1 gene results in the viability of 3% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of the xol-1 gene have been mutated to AUACA and AUAUA, respectively results in the viability of 1% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of one copy of the xol-1 gene have been mutated to AUACA and AUAUA, respectively results in the viability of 7% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG motifs in intron 6 of the xol-1 gene have been mutated to AUACA results in the viability of 24% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG motifs in intron 6 of one copy of the xol-1 gene have been mutated to AUACA results in the viability of 18% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCUAG motifs in intron 6 of the xol-1 gene have been mutated to AUAUA results in the viability of 33% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCUAG motifs in intron 6 of one copy of the xol-1 gene have been mutated to AUAUA results in the viability of 13% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of the xol-1 gene have been mutated to GCUUG results in the viability of 8% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in a strain where all of the GCACG and GCUAG motifs in intron 6 of one copy of the xol-1 gene have been mutated to GCUUG results in the viability of 36% of hermaphrodites (PubMed:33372658).
RNAi-mediated knockdown in strains containing five or three fox-1-binding GCACG motifs in intron 6 of the xol-1 gene results in the viability of 49% and 33% of hermaphrodites, respectively (PubMed:33372658).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000053523 | 1-534 | Steroid hormone receptor family member cnr14 | |||
Sequence: MSFETKPNYLLLTNPDTPLSVCTSPYYSPSGKTASIPSSEASKPEGTNGQWSHLPTGATYVTDEFSSEFQIQNGSTAAQSGNANNYADPLSHRRYFNNVNGYNHHQFYDTASQASVSSPATSVTSSLSPPDSLSNGHTTQRHHIGKAISFCKVCGDKASGYHYGVTSCEGCKGFFRRSIQRKIDYRCLKQQVCEIKRESRNRCQYCRFKKCLDSGMSKDSVRQMKFRNAMRDDKSPDSVFVPEISTLERQEEVDAVYEAVLRAHTTFSFYTDIKIRSIVARPFNVRINEDSKMNRLNAWQIYAHEIDVDIKEVVNFVKEIPKFNFINGNDKAVLLRKNAFPLYLLRIVRGMSNRGLMLRDGRLIDFKSLQLLYGSLADEMLAFANHIITIGCTDGDIALFIVLILCQPLTTEQQFSTNFKSQLQLLEMFDFYKKVLFQKMTCRIDGCDTYKQLMKCIHELNRLNELHKQQLNILRENLSFLNLPPLVVEMFQLSTLPLPVNHNNQENQYTPAPEHQSPQPQQPTPNQQQTPVHC |
Proteomic databases
Expression
Structure
Family & Domains
Features
Showing features for region, zinc finger, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 30-53 | Disordered | ||||
Sequence: SGKTASIPSSEASKPEGTNGQWSH | ||||||
Region | 119-139 | Disordered | ||||
Sequence: PATSVTSSLSPPDSLSNGHTT | ||||||
Zinc finger | 151-171 | NR C4-type | ||||
Sequence: CKVCGDKASGYHYGVTSCEGC | ||||||
Zinc finger | 187-211 | NR C4-type | ||||
Sequence: CLKQQVCEIKRESRNRCQYCRFKKC | ||||||
Domain | 252-493 | NR LBD | ||||
Sequence: EVDAVYEAVLRAHTTFSFYTDIKIRSIVARPFNVRINEDSKMNRLNAWQIYAHEIDVDIKEVVNFVKEIPKFNFINGNDKAVLLRKNAFPLYLLRIVRGMSNRGLMLRDGRLIDFKSLQLLYGSLADEMLAFANHIITIGCTDGDIALFIVLILCQPLTTEQQFSTNFKSQLQLLEMFDFYKKVLFQKMTCRIDGCDTYKQLMKCIHELNRLNELHKQQLNILRENLSFLNLPPLVVEMFQL | ||||||
Region | 502-534 | Disordered | ||||
Sequence: HNNQENQYTPAPEHQSPQPQQPTPNQQQTPVHC |
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length534
- Mass (Da)60,848
- Last updated1996-10-01 v2
- ChecksumE6E888F18750E1B0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U13074 EMBL· GenBank· DDBJ | AAA96982.1 EMBL· GenBank· DDBJ | mRNA | ||
BX284606 EMBL· GenBank· DDBJ | CAA90722.1 EMBL· GenBank· DDBJ | Genomic DNA |