P41270 · NCAP_PUUMP

Function

function

Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable). The nucleocapsid has a left-handed helical structure (By similarity).
As a trimer, specifically binds and acts as a chaperone to unwind the panhandle structure formed by the viral RNA (vRNA) termini (By similarity).
Involved in the transcription and replication initiation of vRNA by mediating primer annealing (By similarity).
Plays a role in cap snatching by sequestering capped RNAs in P bodies for use by the viral RdRp during transcription initiation (By similarity).
Substitutes for the cellular cap-binding complex (eIF4F) to preferentially facilitate the translation of capped mRNAs (By similarity).
Initiates the translation by specifically binding to the cap and 40S ribosomal subunit (By similarity).
Prevents the viral glycoprotein N (Gn) from autophagy-dependent breakdown maybe by blocking autophagosome formation (By similarity).
Inhibits host EIF2AK2/PKR dimerization to prevent PKR-induced translational shutdown in cells and thus the activation of the antiviral state (By similarity).
Also displays sequence-unspecific DNA endonuclease activity (By similarity).

Features

Showing features for site.

143350100150200250300350400
TypeIDPosition(s)Description
Site88Important for the endonuclease activity
Site103Important for the endonuclease activity

GO annotations

AspectTerm
Cellular Componenthelical viral capsid
Cellular Componenthost cell Golgi apparatus
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentribonucleoprotein complex
Cellular Componentviral nucleocapsid
Molecular Functionendonuclease activity
Molecular FunctionRNA binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nucleoprotein
  • EC number
  • Alternative names
    • Nucleocapsid protein (Protein N)

Gene names

    • Name
      N

Organism names

Accessions

  • Primary accession
    P41270

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002220151-433Nucleoprotein

Interaction

Subunit

Homotrimer (By similarity).
Homomultimer (By similarity).
Homomultimerizes and binds to viral genomic RNA to form the nucleocapsid (By similarity).
Interacts with host MAP1LC3B; this interaction participates to the protection of Gn from virus-triggered autophagy (By similarity).
Interacts with host SNAP29; this interaction participates to the protection of glycoprotein N from virus-triggered autophagy (By similarity).
Interacts (via N-terminus) with host RPS19; this interaction probably mediates the loading of the 40S ribosomal subunit on viral capped mRNA during N-mediated translation initiation (By similarity).
Interacts with the viral RdRp (By similarity).
Interacts with host SUMO1 (via N-terminus) (By similarity).
Interacts with host DAXX (By similarity).
Interacts with the viral glycoprotein N (via C-terminus) (By similarity).
Interacts with the viral glycoprotein C (via C-terminus) (By similarity).

Structure

3D structure databases

Family & Domains

Features

Showing features for region, coiled coil, motif.

TypeIDPosition(s)Description
Region1-50RdRP binding
Region1-79Homomultimerization
Region1-100Chaperone activity
Region1-175Viral panhandle binding
Coiled coil4-71
Region69-90Disordered
Region80-248Interaction with glycoprotein N
Region100-125Homomultimerization
Region150-175Interaction with host RPS19
Region175-217Viral RNA-binding
Motif178-181YxxL
Region188-191Interaction with host UBE2I/UBC9
Region377-425Homomultimerization
Region377-433Interaction with host DAXX

Domain

The N-terminus is required for chaperone activity and, in trimeric form, this region likely serves in high affinity vRNA panhandle recognition (By similarity).
The N-terminus also contains a coiled coil region, which probably participates in but is insufficient to initiate N trimerization (By similarity).
The YxxL motif is indispensable for the interaction with host MAP1LC3B (By similarity).
The central region is involved in specific RNA-binding (By similarity).
Has distinct cap- and RNA-binding sites so it can bind simultaneously both the vRNA and mRNA cap (By similarity).

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    433
  • Mass (Da)
    49,518
  • Last updated
    1995-02-01 v1
  • Checksum
    E5E928C93B8FE767
MSDLTDIQEEITRHEQQLVVARQKLKDAERAVEVYPDDVNKNTLQARQQTVSALEDKLADYKRRMADAVSRKKMDTKPTDPTGIEPDDHLKERSSLRYGNVLDVNAIDIEEPSGQTADWYTIGVYVIGFTIPIILKALYMLSTRGRQTVKENKGTRIRFKDDTSFEDINGIRRPKHLYVSMPTAQSTMKAEELTPGRFRTIVCGLFPTQIQVRNIMSPVMGVIGFSFFVKDWPEKIREFMEKECPFIKPEVKPGTPAQEVEFLKRNRVYFMTRQDVLDKNHVADIDKLIDYAASGDPTSPDDIESPNAPWVFACAPDRCPPTCIYVAGMAELGAFFSILQDMRNTIMASKTVGTAEEKLKKKSSFYQSYLRRTQSMGIQLDQRIILLYMLEWGKEMVDHFHLGDDMDPELRGLAQSLIDQKVKEISNQEPLKI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L11347
EMBL· GenBank· DDBJ
AAC37850.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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