P40999 · PMT1M_SCHPO

Function

function

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Can also methylate cytosine 38 in tRNA(Glu), albeit to a lower level, but not tRNA(Lys). Pmt1-dependent tRNA methylation is induced by nitrogen limitation and depends on the nutrient-sensing protein kinase sck2. Does not have DNA-methylation activity.

Caution

Was originally thought not to have cytosine-5 methyltransferase activity, and this was attributed to the insertion of a Ser residue between the Pro-Cys motif found at the active site of C5 MTases (PubMed:8636983).
When this serine is deleted it becomes catalytically active and recognizes and methylates the sequence CC[AT]GG. This was in agreement with S.pombe lacking m5C DNA-methylation. However, it has later been shown that it has tRNA-methyltransferase activity despite this sequence variation (PubMed:23074192).

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site81

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular FunctiontRNA (cytidine) methyltransferase activity
Molecular FunctiontRNA (cytidine-5-)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcesstRNA C5-cytosine methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA (cytosine(38)-C(5))-methyltransferase
  • EC number
  • Alternative names
    • DNA (cytosine-5)-methyltransferase-like protein 2 (Dnmt2)
    • M.SpomI
    • SpIM.SpoI

Gene names

    • Name
      pmt1
    • ORF names
      SPBC19C2.02

Organism names

Accessions

  • Primary accession
    P40999

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis81Loss of methyltransferase activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000880421-330tRNA (cytosine38-C5)-methyltransferase

Proteomic databases

Expression

Induction

Constitutively expressed at protein and RNA levels.

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain7-330SAM-dependent MTase C5-type

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    330
  • Mass (Da)
    37,976
  • Last updated
    1995-02-01 v1
  • Checksum
    50A7121FA7CF58A1
MLSTKRLRVLELYSGIGGMHYALNLANIPADIVCAIDINPQANEIYNLNHGKLAKHMDISTLTAKDFDAFDCKLWTMSPSCQPFTRIGNRKDILDPRSQAFLNILNVLPHVNNLPEYILIENVQGFEESKAAEECRKVLRNCGYNLIEGILSPNQFNIPNSRSRWYGLARLNFKGEWSIDDVFQFSEVAQKEGEVKRIRDYLEIERDWSSYMVLESVLNKWGHQFDIVKPDSSSCCCFTRGYTHLVQGAGSILQMSDHENTHEQFERNRMALQLRYFTAREVARLMGFPESLEWSKSNVTEKCMYRLLGNSINVKVVSYLISLLLEPLNF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X82444
EMBL· GenBank· DDBJ
CAA57824.1
EMBL· GenBank· DDBJ
Genomic DNA
CU329671
EMBL· GenBank· DDBJ
CAB52029.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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