P40830 · PKSG_BACSU
- ProteinPolyketide biosynthesis 3-hydroxy-3-methylglutaryl-ACP synthase PksG
- GenepksG
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids420 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac-AcpK) and a beta-ketothioester polyketide intermediate linked to one of the consecutive thiolation domains of PksL.
Catalytic activity
- 3-oxobutanoyl-[ACP] + acetyl-[ACP] + H2O = (3S)-hydroxy-3-methylglutaryl-[ACP] + holo-[ACP] + H+
Pathway
Antibiotic biosynthesis; bacillaene biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 82 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Active site | 114 | Acyl-thioester intermediate | ||||
Sequence: C | ||||||
Active site | 250 | Proton donor/acceptor | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | hydroxymethylglutaryl-CoA synthase activity | |
Biological Process | acetyl-CoA metabolic process | |
Biological Process | antibiotic biosynthetic process | |
Biological Process | farnesyl diphosphate biosynthetic process, mevalonate pathway |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended namePolyketide biosynthesis 3-hydroxy-3-methylglutaryl-ACP synthase PksG
- EC number
- Short namesHMG synthase
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionP40830
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000213759 | 1-420 | Polyketide biosynthesis 3-hydroxy-3-methylglutaryl-ACP synthase PksG | |||
Sequence: MVSAGIEAMNVFGGTAYLDVMELAKYRHLDTARFENLLMKEKAVALPYEDPVTFGVNAAKPIIDALSEAEKDRIELLITCSESGIDFGKSLSTYIHEYLGLNRNCRLFEVKQACYSGTAGFQMAVNFILSQTSPGAKALVIASDISRFLIAEGGDALSEDWSYAEPSAGAGAVAVLVGENPEVFQIDPGANGYYGYEVMDTCRPIPDSEAGDSDLSLMSYLDCCEQTFLEYQKRVPGANYQDTFQYLAYHTPFGGMVKGAHRTMMRKVAKVKTSGIETDFLTRVKPGLNYCQRVGNIMGAALFLALASTIDQGRFDTPKRIGCFSYGSGCCSEFYSGITTPQGQERQRTFGIEKHLDRRYQLSMEEYELLFKGSGMVRFGTRNVKLDFEMIPGIMQSTQEKPRLFLEEISEFHRKYRWIS |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the thiolase-like superfamily. HMG-CoA synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length420
- Mass (Da)46,780
- Last updated2004-03-29 v2
- Checksum8808BDDFEEAC8F70
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U11039 EMBL· GenBank· DDBJ | AAA85140.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AL009126 EMBL· GenBank· DDBJ | CAB13586.2 EMBL· GenBank· DDBJ | Genomic DNA |