P40685 · CHRR_CERS4

Function

function

Anti-sigma factor that inhibits the activity of the extracytoplasmic function (ECF) sigma-E factor (RpoE), thereby indirectly regulating the transcription of the cycA and rpoE genes. ECF sigma factors are held in an inactive form by a cognate anti-sigma factor.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ion per subunit. The Zn2+ bound by the N-terminus is required for anti-sigma function, the function of the Zn2+ bound by the C-terminus is unknown.

Features

Showing features for binding site.

121320406080100120140160180200
TypeIDPosition(s)Description
Binding site6Zn2+ 1 (UniProtKB | ChEBI)
Binding site31Zn2+ 1 (UniProtKB | ChEBI)
Binding site35Zn2+ 1 (UniProtKB | ChEBI)
Binding site38Zn2+ 1 (UniProtKB | ChEBI)
Binding site141Zn2+ 2 (UniProtKB | ChEBI)
Binding site143Zn2+ 2 (UniProtKB | ChEBI)
Binding site147Zn2+ 2 (UniProtKB | ChEBI)
Binding site177Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular Functionmetal ion binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Anti-sigma-E factor ChrR
  • Alternative names
    • Sigma-E anti-sigma factor ChrR
    • Transcriptional activator ChrR

Gene names

    • Name
      chrR
    • ORF names
      RSP_1093
    • Ordered locus names
      RHOS4_27110

Organism names

Accessions

  • Primary accession
    P40685
  • Secondary accessions
    • Q3IYV5

Proteomes

Phenotypes & Variants

Disruption phenotype

For single chrR mutant about 12-fold increase in rpoE-regulated genes. For double rpoE-chrR deletion mutant no effect on anaerobic photosynthetic growth. In illuminated aerobically growing cells double deletion is bacteriostatic.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis5No effect on anti-sigma function.
Mutagenesis6Loss of anti-sigma function.
Mutagenesis31No effect on anti-sigma function.
Mutagenesis35Loss of anti-sigma function.
Mutagenesis35Loss of function; no effect on zinc binding.
Mutagenesis38Loss of anti-sigma function.
Mutagenesis38In Chr4 mutant; loss of function.
Mutagenesis38Loss of ability to bind zinc.
Mutagenesis187No effect on zinc binding.
Mutagenesis189No effect on zinc binding.

PTM/Processing

Features

Showing features for initiator methionine, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000896582-213Anti-sigma-E factor ChrR

Expression

Induction

Induced by singlet oxygen. Autoregulated. Part of the rpoE-chrR operon.

Interaction

Subunit

Forms a 1:1 complex with cognate ECF RNA polymerase sigma factor RpoE; this inhibits the interaction of RpoE with the RNA polymerase catalytic core.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region2-85Sufficient to bind sigma factor and inhibit its activity
Region86-194Required for response to singlet oxygen

Domain

The N-terminal anti-sigma domain (residues 1-85) is necessary and sufficient to bind sigma-E and inhibit its activity. The C-terminal domain (residues 86-194) is required to respond to singlet oxygen (PubMed:17803943).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    213
  • Mass (Da)
    22,865
  • Last updated
    2007-01-23 v4
  • Checksum
    46152BC5858C845F
MTIRHHVSDALLTAYAAGTLSEAFSLVVATHLSLCDECRARAGALDAVGGSLMEETAPVALSEGSLASVMAQLDRQIQRPAPARRADPRAPAPLADYVGRRLEDVRWRTLGGGVRQAILPTGGEAIARLLWIPGGQAVPDHGHRGLELTLVLQGAFRDETDRFGAGDIEIADQELEHTPVAERGLDCICLAATDAPLRFNSFLPKLVQPFFRI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U11283
EMBL· GenBank· DDBJ
AAB17905.1
EMBL· GenBank· DDBJ
Genomic DNA
CP000143
EMBL· GenBank· DDBJ
ABA80279.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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