P40487 · DPH1_YEAST

Function

function

Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed:15485916, PubMed:24422557, PubMed:27694803, PubMed:31463593, PubMed:34154323).
In association with DPH2, transfers a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising KTI11/DPH3 and a NADH-dependent reductase, predominantly CBR1 (PubMed:15485916, PubMed:24422557, PubMed:27694803, PubMed:31463593, PubMed:34154323).

Miscellaneous

Present with 2526 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Protein modification; peptidyl-diphthamide biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site133[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site239[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site368[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Component2-(3-amino-3-carboxypropyl)histidine synthase complex
Cellular Componentcytoplasm
Molecular Function2-(3-amino-3-carboxypropyl)histidine synthase activity
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Biological Processprotein histidyl modification to diphthamide

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
  • EC number
  • Alternative names
    • Diphthamide biosynthesis protein 1
    • Diphtheria toxin resistance protein 1
    • S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1

Gene names

    • Name
      DPH1
    • Ordered locus names
      YIL103W

Organism names

Accessions

  • Primary accession
    P40487
  • Secondary accessions
    • D6VVI4

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Increases translational -1 frameshifting (PubMed:18627462).
Abolishes the formation of the 2-(3-amino-3-carboxypropyl)histidine synthase complex (PubMed:18627462).
Resistance to sordarin and zymocin (PubMed:18627462).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis133Resistance to diphtheria toxin.
Mutagenesis239Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and catalytic activity; when associated with S-368.
Mutagenesis368Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and catalytic activity; when associated with S-239.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00000833811-4252-(3-amino-3-carboxypropyl)histidine synthase subunit 1
Modified residue44Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, KTI11/DPH3 and a NADH-dependent reductase, predominantly CBR1 (PubMed:15485916, PubMed:18627462, PubMed:23645155, PubMed:24422557, PubMed:27694803, PubMed:31463593).
Interacts with DPH2; the interaction is direct (PubMed:15485916, PubMed:23645155, PubMed:31463593).
Interacts with KTI11/DPH3 (PubMed:12940988, PubMed:18627462, PubMed:23645155, PubMed:27694803).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY P40487DPH2 P324614EBI-25162, EBI-6090
BINARY P40487KTI11 Q3E8402EBI-25162, EBI-2055307

Complex viewer

View interactors in UniProtKB
View CPX-2581 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-29Disordered
Region1-60Required for function but dispensable for interaction with DPH2 and DPH3
Region366-425Required for function but dispensable for interaction with DPH2 and DPH3

Sequence similarities

Belongs to the DPH1/DPH2 family. DPH1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    425
  • Mass (Da)
    48,311
  • Last updated
    1995-02-01 v1
  • Checksum
    22E93852E03C6722
MSGSTESKKQPRRRFIGRKSGNSNNDKLTTVAENGNEIIHKQKSRIALGRSVNHVPEDILNDKELNEAIKLLPSNYNFEIHKTVWNIRKYNAKRIALQMPEGLLIYSLIISDILEQFCGVETLVMGDVSYGACCIDDFTARALDCDFIVHYAHSCLVPIDVTKIKVLYVFVTINIQEDHIIKTLQKNFPKGSRIATFGTIQFNPAVHSVRDKLLNDEEHMLYIIPPQIKPLSRGEVLGCTSERLDKEQYDAMVFIGDGRFHLESAMIHNPEIPAFKYDPYNRKFTREGYDQKQLVEVRAEAIEVARKGKVFGLILGALGRQGNLNTVKNLEKNLIAAGKTVVKIILSEVFPQKLAMFDQIDVFVQVACPRLSIDWGYAFNKPLLTPYEASVLLKKDVMFSEKYYPMDYYEAKGYGRGETPKHAIE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z38125
EMBL· GenBank· DDBJ
CAA86277.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006942
EMBL· GenBank· DDBJ
DAA08450.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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