P40484 · MOB1_YEAST
- ProteinDBF2 kinase activator protein MOB1
- GeneMOB1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids314 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Functions as an activator subunit for the DBF2 protein kinase. Binds to DBF2, which is required for the phosphorylation and activation of DBF2 by the upstream kinase CDC15 in late anaphase. DBF2-MOB1 is part of the mitotic exit network (MEN) signaling cascade, which regulates release from the nucleus and activity of phosphatase CDC14. Required for inactivation of mitotic cyclin-dependent kinase for exit from mitosis, cytokinesis and G1 gene transcription.
Miscellaneous
Present with 5020 molecules/cell in log phase SD medium.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cellular bud neck | |
Cellular Component | chromosome, centromeric region | |
Cellular Component | cytoplasm | |
Cellular Component | mitotic spindle pole body | |
Cellular Component | nuclear periphery | |
Cellular Component | nucleus | |
Cellular Component | serine/threonine protein kinase complex | |
Cellular Component | Sid2-Mob1 complex | |
Cellular Component | spindle pole body | |
Molecular Function | kinase regulator activity | |
Molecular Function | metal ion binding | |
Molecular Function | protein kinase activator activity | |
Biological Process | cell division | |
Biological Process | exit from mitosis | |
Biological Process | mitotic cytokinesis | |
Biological Process | positive regulation of protein phosphorylation | |
Biological Process | regulation of cytokinesis | |
Biological Process | regulation of exit from mitosis | |
Biological Process | signal transduction |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDBF2 kinase activator protein MOB1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP40484
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes predominantly to the cytoplasmic surface of the spindle pole body during anaphase and to a ring at the bud neck during cytokinesis. Translocates to the nucleus during mitosis and associates with centromere DNA.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 105 | No effect. | ||||
Sequence: T → A or D | ||||||
Mutagenesis | 107 | No effect. | ||||
Sequence: S → A or D |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000193582 | 1-314 | DBF2 kinase activator protein MOB1 | |||
Sequence: MSFLQNFHISPGQTIRSTRGFKWNTANAANNAGSVSPTKATPHNNTINGNNNNANTINNRADFTNNPVNGYNESDHGRMSPVLTTPKRHAPPPEQLQNVTDFNYTPSHQKPFLQPQAGTTVTTHQDIKQIVEMTLGSEGVLNQAVKLPRGEDENEWLAVHCVDFYNQINMLYGSITEFCSPQTCPRMIATNEYEYLWAFQKGQPPVSVSAPKYVECLMRWCQDQFDDESLFPSKVTGTFPEGFIQRVIQPILRRLFRVYAHIYCHHFNEILELNLQTVLNTSFRHFCLFAQEFELLRPADFGPLLELVMELRDR | ||||||
Modified residue | 34 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 36 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 80 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 229 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated by CDC15.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with protein kinase DBF2 to form the MEN DBF2-MOB1 kinase complex.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P40484 | DBF2 P22204 | 16 | EBI-11119, EBI-5569 | |
BINARY | P40484 | DBF20 P32328 | 8 | EBI-11119, EBI-5588 | |
BINARY | P40484 | POP2 P39008 | 2 | EBI-11119, EBI-13629 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 29-55 | Disordered | ||||
Sequence: ANNAGSVSPTKATPHNNTINGNNNNAN |
Sequence similarities
Belongs to the MOB1/phocein family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length314
- Mass (Da)35,882
- Last updated2006-10-17 v2
- Checksum0B50B1D18D7EF54A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z38125 EMBL· GenBank· DDBJ | CAA86274.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006942 EMBL· GenBank· DDBJ | DAA08447.1 EMBL· GenBank· DDBJ | Genomic DNA |