P40343 · VPS27_YEAST

Function

function

Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane (PubMed:11416128, PubMed:11872141, PubMed:12055639, PubMed:12900393, PubMed:14581452, PubMed:1493335, PubMed:15086794, PubMed:15107463, PubMed:15166140, PubMed:17135292, PubMed:18508771, PubMed:3062374, PubMed:9265642).
Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi (PubMed:11208109, PubMed:8649377, PubMed:9015300).
Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC (PubMed:8649377).
Might also function as an alternate adapter in the COPIb clathrin-like coat (PubMed:17101773).

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.

Features

Showing features for binding site.

162250100150200250300350400450500550600
TypeIDPosition(s)Description
Binding site176Zn2+ 1 (UniProtKB | ChEBI)
Binding site179Zn2+ 1 (UniProtKB | ChEBI)
Binding site192Zn2+ 2 (UniProtKB | ChEBI)
Binding site195Zn2+ 2 (UniProtKB | ChEBI)
Binding site200Zn2+ 1 (UniProtKB | ChEBI)
Binding site203Zn2+ 1 (UniProtKB | ChEBI)
Binding site222Zn2+ 2 (UniProtKB | ChEBI)
Binding site225Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentendosome
Cellular Componentendosome membrane
Cellular ComponentESCRT-0 complex
Cellular Componentprotein-containing complex
Cellular Componentvacuolar membrane
Molecular FunctionK48-linked polyubiquitin modification-dependent protein binding
Molecular FunctionK63-linked polyubiquitin modification-dependent protein binding
Molecular Functionmetal ion binding
Molecular Functionphosphatidylinositol-3-phosphate binding
Molecular Functionprotein domain specific binding
Molecular Functionprotein heterodimerization activity
Molecular Functionubiquitin binding
Biological ProcessATP export
Biological Processcellular response to nitrogen starvation
Biological Processlate endosome to vacuole transport
Biological Processmicroautophagy
Biological Processmicrolipophagy
Biological Processmultivesicular body sorting pathway
Biological Processpositive regulation of protein maturation
Biological Processprotein retention in Golgi apparatus
Biological Processprotein secretion
Biological Processprotein targeting to vacuole
Biological Processprotein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Biological Processubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

Keywords

Enzyme and pathway databases

Protein family/group databases

    • 3.A.31.1.1the endosomal sorting complexes required for transport iii (escrt-iii) family

Names & Taxonomy

Protein names

  • Recommended name
    Vacuolar protein sorting-associated protein 27
  • Alternative names
    • Golgi retention defective protein 11

Gene names

    • Name
      VPS27
    • Synonyms
      DID7, GRD11, SSV17, VPL23, VPT27
    • ORF names
      N2038
    • Ordered locus names
      YNR006W

Organism names

Accessions

  • Primary accession
    P40343
  • Secondary accessions
    • D6W1I1

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis185-186Decreases the association to PtdIns3P containing membranes.
Mutagenesis193Decreases the association to PtdIns3P containing membranes.
Mutagenesis270Strongly reduces the ubiquitin-binding activity.
Mutagenesis313Strongly reduces the ubiquitin-binding activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 13 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue, cross-link.

TypeIDPosition(s)Description
ChainPRO_00000658931-622Vacuolar protein sorting-associated protein 27
Modified residue157Phosphoserine
Cross-link294Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residue495Phosphoserine
Modified residue613Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Component of the ESCRT-0 complex composed of HSE1 and VPS27 (PubMed:12055639, PubMed:14581452, PubMed:15086794).
Interacts with ENT3 and ENT5, the ESCRT-I subunits VPS23 and VPS28 and with the COPIb subunits SEC27, SEC28 and SEC33 (PubMed:12900393, PubMed:15107463, PubMed:17101773).
May form a complex composed of VPS27, HSE1 and DOA1 (PubMed:18508771).
Interacts with DOA1 (PubMed:18508771).
Interacts with ubiquitin (PubMed:11988742, PubMed:12750381, PubMed:14581452).

Binary interactions

Complex viewer

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, zinc finger, region, compositional bias.

TypeIDPosition(s)Description
Domain18-149VHS
Zinc finger170-230FYVE-type; atypical
Region236-260Disordered
Domain258-277UIM 1
Domain301-320UIM 2
Region317-348Disordered
Compositional bias462-520Polar residues
Region462-622Disordered

Domain

The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns3P) which is required for the association to endosomal membranes.
Both IUM domains are necessary for efficient binding to ubiquitin.

Sequence similarities

Belongs to the VPS27 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    622
  • Mass (Da)
    70,974
  • Last updated
    2010-10-05 v3
  • Checksum
    76C4501B0EBC9C84
MSVSTPSELDALIEQATSESIPNGDLDLPIALEISDVLRSRRVNPKDSMRCIKKRILNTADNPNTQLSSWKLTNICVKNGGTPFIKEICSREFMDTMEHVILREDSNEELSELVKTILYELYVAFKNDSQLNYVAKVYDKLISRGIKFPEKLTLSNSPTAMFDSKTPADWIDSDACMICSKKFSLLNRKHHCRSCGGVFCQEHSSNSIPLPDLGIYEPVRVCDSCFEDYDLKRHDDSKKSKKHRHKRKKDRDYSTPEDEEELIRKAIELSLKESRNSASSEPIVPVVESKNEVKRQEIEEEEDPDLKAAIQESLREAEEAKLRSERQKASRQMQPQQPSPQPQPIHSVDLSDEEKDSIYMFASLVEKMKSRPLNEILEDSKLQNLAQRVFASKARLNYALNDKAQKYNTLIEMNGKISEIMNIYDRLLEQQLQSINLSQQYTLPQVPSDPYNYLTENVQNPAESYQTPPLQQLSSHQYKPQQDVSRQQSVKANSSPTTNIDHLKTIDVTPHAQQKPQSHVELAPSDPPYPKEEAEDEGTQAVQDEESSTQESRERPYPVETENGETSINKRPQGITRYDFPTVPARKFVQPESTVPLPASSSEIPIKEERPPSPQEELLIEL

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict321-322in Ref. 1; AAA96002
Compositional bias462-520Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U24218
EMBL· GenBank· DDBJ
AAA96002.1
EMBL· GenBank· DDBJ
Genomic DNA
X77395
EMBL· GenBank· DDBJ
CAA54574.1
EMBL· GenBank· DDBJ
Genomic DNA
Z71620
EMBL· GenBank· DDBJ
CAA96282.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006947
EMBL· GenBank· DDBJ
DAA10547.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp