P40096 · NCB1_YEAST

Function

function

Component of the NC2 complex which represses RNA polymerase II transcription through binding to SPT15/TBP and thereby inhibiting the assembly of the preinitiation complex. The NC2 complex may also mediate transcriptional activation from TATA-driven promoters through association with SPT15/TBP.

Miscellaneous

Present with 5130 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnegative cofactor 2 complex
Cellular Componentnucleus
Molecular Functionchromatin binding
Molecular Functioncore promoter sequence-specific DNA binding
Molecular Functionprotein heterodimerization activity
Molecular FunctionRNA polymerase II general transcription initiation factor activity
Molecular Functiontranscription coactivator activity
Molecular Functiontranscription corepressor activity
Biological Processcellular response to heat
Biological Processnegative regulation of RNA polymerase II transcription preinitiation complex assembly
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processpositive regulation of transcription by RNA polymerase II
Biological ProcessRNA polymerase II preinitiation complex assembly
Biological Processtranscription by RNA polymerase II

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Negative cofactor 2 complex subunit alpha
  • Short names
    NC2 complex subunit alpha
  • Alternative names
    • Transcription repressor BUR6

Gene names

    • Name
      BUR6
    • Synonyms
      NCB1
    • Ordered locus names
      YER159C

Organism names

Accessions

  • Primary accession
    P40096
  • Secondary accessions
    • D3DM66

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000967551-142Negative cofactor 2 complex subunit alpha
Modified residue27Phosphoserine
Modified residue141Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Component of the NC2 (negative cofactor 2) complex composed of BUR6 and NCB2. The NC2 complex associates with SPT15/TBP. Interacts with SPT15/TBP.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P40096NCB2 Q923176EBI-11908, EBI-37723
BINARY P40096SPT15 P133932EBI-11908, EBI-19129

Complex viewer

View interactors in UniProtKB
View CPX-1662 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-43Disordered
Compositional bias29-43Polar residues
Domain29-137Histone-fold

Sequence similarities

Belongs to the NC2 alpha/DRAP1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    142
  • Mass (Da)
    15,517
  • Last updated
    1995-02-01 v1
  • Checksum
    27341BD250973BA7
MADQVPVTTQLPPIKPEHEVPLDAGGSPVGNMGTNSNNNNELGDVFDRIKTHFPPAKVKKIMQTDEDIGKVSQATPVIAGRSLEFFIALLVKKSGEMARGQGTKRITAEILKKTILNDEKFDFLREGLCVEEGQTQPEEESA

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias29-43Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Y09265
EMBL· GenBank· DDBJ
CAA70460.1
EMBL· GenBank· DDBJ
mRNA
U18917
EMBL· GenBank· DDBJ
AAB64686.1
EMBL· GenBank· DDBJ
Genomic DNA
AY693203
EMBL· GenBank· DDBJ
AAT93222.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006939
EMBL· GenBank· DDBJ
DAA07820.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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