P40028 · YEN1_YEAST

Function

function

Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Involved in DNA-damage repair in vegetative cells.

Miscellaneous

Present with 131 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular Function5'-flap endonuclease activity
Molecular Functioncrossover junction DNA endonuclease activity
Molecular Functiondouble-stranded DNA 5'-3' DNA exonuclease activity
Molecular Functionfour-way junction DNA binding
Molecular Functionmagnesium ion binding
Molecular Functionprotein homodimerization activity
Biological ProcessDNA damage response
Biological ProcessDNA repair
Biological Processreplication fork processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Holliday junction resolvase YEN1
  • EC number

Gene names

    • Name
      YEN1
    • Ordered locus names
      YER041W

Organism names

Accessions

  • Primary accession
    P40028
  • Secondary accessions
    • D3DLU1
    • Q02463

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Note: Predominantly nuclear in G1-arrested cells, but cytoplasmically localized after release from G1 arrest.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis41Mutants do not survive after treatment with DNA-damaging agent MMS.
Mutagenesis97Mutants show a slight growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis298Mutants show a very strong growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis469Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis478Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis484Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis486Mutants show a strong growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis487Mutants show a strong growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis526Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis528Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis529Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutagenesis530Mutants show no effect after treatment with DNA-damaging agent MMS.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 31 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002026291-759Holliday junction resolvase YEN1
Modified residue730Phosphoserine
Modified residue731Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region62-83Disordered
Compositional bias498-515Polar residues
Region498-518Disordered
Region683-702Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    759
  • Mass (Da)
    87,364
  • Last updated
    2011-07-27 v2
  • Checksum
    CE1305A9F78CA300
MGVSQIWEFLKPYLQDSRIPLRKFVIDFNKSQKRAPRIAIDAYGWLFECGFIQNIDISARSRSRSRSPTRSPRDSDIDSSQEYYGSRSYTTTGKAVINFISRLKELLSLNVEFLLVFDGVMKPSFKRKFNHEQNATTCDDEKEYYSSWEQHVKNHEVYGNCKGLLAPSDPEFISLVRKLLDLMNISYVIACGEGEAQCVWLQVSGAVDFILSNDSDTLVFGGEKILKNYSKFYDDFGPSSITSHSPSRHHDSKESFVTVIDLPKINKVAGKKFDRLSLLFFSVLLGADYNRGVKGLGKNKSLQLAQCEDPNFSMEFYDIFKDFNLEDLTSESLRKSRYRLFQKRLYLYCKDHSVELFGRNYPVLLNQGSFEGWPSTVAIMHYFHPIVQPYFDEEVLSDKYINMAGNGHYRNLNFNELKYFLQSLNLPQISSFDKWFHDSMHEMFLLREFLSIDESDNIGKGNMRITEEKIMNIDGGKFQIPCFKIRYTTFLPNIPISSQSPLKRSNSPSRSKSPTRRQMDIMEHPNSLWLPKYLIPQSHPLVIQYYETQQLIQKEKEKKGKKSNKSRLPQKNNLDEFLRKHTSPIKSIGKVGESRKEILEPVRKRLFVDTDEDTSLEEIPAPTRLTTVDEHSDNDDDSLIFVDEITNSQSVLDSSPGKRIRDLTQDEQVDVWKDVIEISPIKKSRTTNAEKNPPESGLKSRSSITINARLQGTKMLPPNLTAPRLEREHSSVLDQLVTDAQDTVDRFVACDSDSSSTIE

Sequence caution

The sequence AAA60376.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict59in Ref. 2; AAB64576
Sequence conflict164-165in Ref. 1; AAA60376
Sequence conflict346in Ref. 1; AAA60376
Compositional bias498-515Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U13615
EMBL· GenBank· DDBJ
AAA60376.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
U18796
EMBL· GenBank· DDBJ
AAB64576.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006939
EMBL· GenBank· DDBJ
DAA07695.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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