P40028 · YEN1_YEAST
- ProteinHolliday junction resolvase YEN1
- GeneYEN1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids759 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Involved in DNA-damage repair in vegetative cells.
Miscellaneous
Present with 131 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | 5'-flap endonuclease activity | |
Molecular Function | crossover junction DNA endonuclease activity | |
Molecular Function | double-stranded DNA 5'-3' DNA exonuclease activity | |
Molecular Function | four-way junction DNA binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | protein homodimerization activity | |
Biological Process | DNA damage response | |
Biological Process | DNA repair | |
Biological Process | replication fork processing |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHolliday junction resolvase YEN1
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP40028
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 41 | Mutants do not survive after treatment with DNA-damaging agent MMS. | ||||
Sequence: D → N | ||||||
Mutagenesis | 97 | Mutants show a slight growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: I → E | ||||||
Mutagenesis | 298 | Mutants show a very strong growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: K → E | ||||||
Mutagenesis | 469 | Mutants show a growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: K → E | ||||||
Mutagenesis | 478 | Mutants show a growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: F → A | ||||||
Mutagenesis | 484 | Mutants show a growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: K → E | ||||||
Mutagenesis | 486 | Mutants show a strong growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: R → E | ||||||
Mutagenesis | 487 | Mutants show a strong growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 526 | Mutants show a growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: N → A | ||||||
Mutagenesis | 528 | Mutants show a growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: L → D | ||||||
Mutagenesis | 529 | Mutants show a growth defect after treatment with DNA-damaging agent MMS. | ||||
Sequence: W → A | ||||||
Mutagenesis | 530 | Mutants show no effect after treatment with DNA-damaging agent MMS. | ||||
Sequence: L → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 31 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000202629 | 1-759 | Holliday junction resolvase YEN1 | |||
Sequence: MGVSQIWEFLKPYLQDSRIPLRKFVIDFNKSQKRAPRIAIDAYGWLFECGFIQNIDISARSRSRSRSPTRSPRDSDIDSSQEYYGSRSYTTTGKAVINFISRLKELLSLNVEFLLVFDGVMKPSFKRKFNHEQNATTCDDEKEYYSSWEQHVKNHEVYGNCKGLLAPSDPEFISLVRKLLDLMNISYVIACGEGEAQCVWLQVSGAVDFILSNDSDTLVFGGEKILKNYSKFYDDFGPSSITSHSPSRHHDSKESFVTVIDLPKINKVAGKKFDRLSLLFFSVLLGADYNRGVKGLGKNKSLQLAQCEDPNFSMEFYDIFKDFNLEDLTSESLRKSRYRLFQKRLYLYCKDHSVELFGRNYPVLLNQGSFEGWPSTVAIMHYFHPIVQPYFDEEVLSDKYINMAGNGHYRNLNFNELKYFLQSLNLPQISSFDKWFHDSMHEMFLLREFLSIDESDNIGKGNMRITEEKIMNIDGGKFQIPCFKIRYTTFLPNIPISSQSPLKRSNSPSRSKSPTRRQMDIMEHPNSLWLPKYLIPQSHPLVIQYYETQQLIQKEKEKKGKKSNKSRLPQKNNLDEFLRKHTSPIKSIGKVGESRKEILEPVRKRLFVDTDEDTSLEEIPAPTRLTTVDEHSDNDDDSLIFVDEITNSQSVLDSSPGKRIRDLTQDEQVDVWKDVIEISPIKKSRTTNAEKNPPESGLKSRSSITINARLQGTKMLPPNLTAPRLEREHSSVLDQLVTDAQDTVDRFVACDSDSSSTIE | ||||||
Modified residue | 730 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 731 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 62-83 | Disordered | ||||
Sequence: RSRSRSPTRSPRDSDIDSSQEY | ||||||
Compositional bias | 498-515 | Polar residues | ||||
Sequence: SQSPLKRSNSPSRSKSPT | ||||||
Region | 498-518 | Disordered | ||||
Sequence: SQSPLKRSNSPSRSKSPTRRQ | ||||||
Region | 683-702 | Disordered | ||||
Sequence: KSRTTNAEKNPPESGLKSRS |
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length759
- Mass (Da)87,364
- Last updated2011-07-27 v2
- ChecksumCE1305A9F78CA300
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 59 | in Ref. 2; AAB64576 | ||||
Sequence: A → P | ||||||
Sequence conflict | 164-165 | in Ref. 1; AAA60376 | ||||
Sequence: LL → FV | ||||||
Sequence conflict | 346 | in Ref. 1; AAA60376 | ||||
Sequence: Y → C | ||||||
Compositional bias | 498-515 | Polar residues | ||||
Sequence: SQSPLKRSNSPSRSKSPT |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U13615 EMBL· GenBank· DDBJ | AAA60376.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
U18796 EMBL· GenBank· DDBJ | AAB64576.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006939 EMBL· GenBank· DDBJ | DAA07695.2 EMBL· GenBank· DDBJ | Genomic DNA |