P39976 · DLD3_YEAST

Function

function

Catalyzes the reversible oxidation of (R)-2-hydroxyglutarate to 2-oxoglutarate coupled to reduction of pyruvate to (R)-lactate. Can also use oxaloacetate as electron acceptor instead of pyruvate producing (R)-malate.

Miscellaneous

Present with 13000 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
111 μM(R)-2-hydroxyglutarate
450 μMpyruvate
533 μM(R)-lactate
kcat is 4.0 sec-1 for (R)-2-hydroxyglutarate oxidation with pyruvate as electron acceptor. kcat is 6.6 sec-1 for (R)-2-hydroxyglutarate oxidation with DCIP as electron acceptor. kcat is 4.9 sec-1 for pyruvate reduction with (R)-2-hydroxyglutarate as electron donor. kcat is 16.5 sec-1 for (R)-lactate reduction with DCIP as electron donor.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentmitochondrion
Molecular Function(D)-2-hydroxyglutarate-pyruvate transhydrogenase activity
Molecular FunctionD-lactate dehydrogenase (cytochrome) activity
Molecular FunctionFAD binding
Biological Processfilamentous growth
Biological Processlactate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3
  • EC number
  • Short names
    D-2HG--pyruvate transhydrogenase DLD3
  • Alternative names
    • (R)-2-hydroxyglutarate--pyruvate transhydrogenase
    • D-lactate dehydrogenase [cytochrome] 3 (EC:1.1.2.4) . EC:1.1.2.4 (UniProtKB | ENZYME | Rhea)
    • D-lactate ferricytochrome C oxidoreductase (D-LCR)

Gene names

    • Name
      DLD3
    • Ordered locus names
      YEL071W

Organism names

Accessions

  • Primary accession
    P39976
  • Secondary accessions
    • D3DLI0

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, cross-link.

TypeIDPosition(s)Description
ChainPRO_00001281761-496D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3
Cross-link17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Keywords

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain64-243FAD-binding PCMH-type

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    496
  • Mass (Da)
    55,225
  • Last updated
    1995-02-01 v1
  • Checksum
    4809F74EDF07F520
MTAAHPVAQLTAEAYPKVKRNPNFKVLDSEDLAYFRSILSNDEILNSQAPEELASFNQDWMKKYRGQSNLILLPNSTDKVSKIMKYCNDKKLAVVPQGGNTDLVGASVPVFDEIVLSLRNMNKVRDFDPVSGTFKCDAGVVMRDAHQFLHDHDHIFPLDLPSRNNCQVGGVVSTNAGGLNFLRYGSLHGNVLGLEVVLPNGEIISNINALRKDNTGYDLKQLFIGAEGTIGVVTGVSIVAAAKPKALNAVFFGIENFDTVQKLFVKAKSELSEILSAFEFMDRGSIECTIEYLKDLPFPLENQHNFYVLIETSGSNKRHDDEKLTAFLKDTTDSKLISEGMMAKDKADFDRLWTWRKSVPTACNSYGGMYKYDMSLQLKDLYSVSAAVTERLNAAGLIGDAPKPVVKSCGYGHVGDGNIHLNIAVREFTKQIEDLLEPFVYEYIASKKGSISAEHGIGFHKKGKLHYTRSDIEIRFMKDIKNHYDPNGILNPYKYI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U18795
EMBL· GenBank· DDBJ
AAB65016.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006939
EMBL· GenBank· DDBJ
DAA07584.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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