P39976 · DLD3_YEAST
- ProteinD-2-hydroxyglutarate--pyruvate transhydrogenase DLD3
- GeneDLD3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids496 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the reversible oxidation of (R)-2-hydroxyglutarate to 2-oxoglutarate coupled to reduction of pyruvate to (R)-lactate. Can also use oxaloacetate as electron acceptor instead of pyruvate producing (R)-malate.
Miscellaneous
Present with 13000 molecules/cell in log phase SD medium.
Catalytic activity
- (R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + 2 H+ + pyruvate
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
111 μM | (R)-2-hydroxyglutarate | |||||
450 μM | pyruvate | |||||
533 μM | (R)-lactate |
kcat is 4.0 sec-1 for (R)-2-hydroxyglutarate oxidation with pyruvate as electron acceptor. kcat is 6.6 sec-1 for (R)-2-hydroxyglutarate oxidation with DCIP as electron acceptor. kcat is 4.9 sec-1 for pyruvate reduction with (R)-2-hydroxyglutarate as electron donor. kcat is 16.5 sec-1 for (R)-lactate reduction with DCIP as electron donor.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mitochondrion | |
Molecular Function | (D)-2-hydroxyglutarate-pyruvate transhydrogenase activity | |
Molecular Function | D-lactate dehydrogenase (cytochrome) activity | |
Molecular Function | FAD binding | |
Biological Process | filamentous growth | |
Biological Process | lactate biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-2-hydroxyglutarate--pyruvate transhydrogenase DLD3
- EC number
- Short namesD-2HG--pyruvate transhydrogenase DLD3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP39976
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000128176 | 1-496 | D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3 | |||
Sequence: MTAAHPVAQLTAEAYPKVKRNPNFKVLDSEDLAYFRSILSNDEILNSQAPEELASFNQDWMKKYRGQSNLILLPNSTDKVSKIMKYCNDKKLAVVPQGGNTDLVGASVPVFDEIVLSLRNMNKVRDFDPVSGTFKCDAGVVMRDAHQFLHDHDHIFPLDLPSRNNCQVGGVVSTNAGGLNFLRYGSLHGNVLGLEVVLPNGEIISNINALRKDNTGYDLKQLFIGAEGTIGVVTGVSIVAAAKPKALNAVFFGIENFDTVQKLFVKAKSELSEILSAFEFMDRGSIECTIEYLKDLPFPLENQHNFYVLIETSGSNKRHDDEKLTAFLKDTTDSKLISEGMMAKDKADFDRLWTWRKSVPTACNSYGGMYKYDMSLQLKDLYSVSAAVTERLNAAGLIGDAPKPVVKSCGYGHVGDGNIHLNIAVREFTKQIEDLLEPFVYEYIASKKGSISAEHGIGFHKKGKLHYTRSDIEIRFMKDIKNHYDPNGILNPYKYI | ||||||
Cross-link | 17 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 64-243 | FAD-binding PCMH-type | ||||
Sequence: YRGQSNLILLPNSTDKVSKIMKYCNDKKLAVVPQGGNTDLVGASVPVFDEIVLSLRNMNKVRDFDPVSGTFKCDAGVVMRDAHQFLHDHDHIFPLDLPSRNNCQVGGVVSTNAGGLNFLRYGSLHGNVLGLEVVLPNGEIISNINALRKDNTGYDLKQLFIGAEGTIGVVTGVSIVAAAK |
Sequence similarities
Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length496
- Mass (Da)55,225
- Last updated1995-02-01 v1
- Checksum4809F74EDF07F520
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U18795 EMBL· GenBank· DDBJ | AAB65016.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006939 EMBL· GenBank· DDBJ | DAA07584.1 EMBL· GenBank· DDBJ | Genomic DNA |