P39968 · VAC8_YEAST

Function

function

Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole (cvt) (PubMed:9490720).
Involved in the formation of nucleus-vacuole junctions (NVJs) during piecemeal microautophagy of the nucleus (PMN) (PubMed:10888680, PubMed:12529432, PubMed:16912077).
NVJs are interorganelle interfaces mediated by NVJ1 in the nuclear envelope and VAC8 on the vacuole membrane (PubMed:10888680, PubMed:12529432, PubMed:16912077).
Together, NVJ1 and VAC8 form Velcro-like patches through which teardrop-like portions of the nucleus are pinched off into the vacuolar lumen and degraded by the PMN process (PubMed:10888680, PubMed:12529432, PubMed:16912077).

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentfungal-type vacuole membrane
Cellular Componentmembrane
Cellular Componentmembrane raft
Cellular ComponentMyo2p-Vac17p-Vac8p transport complex
Cellular Componentnuclear membrane
Cellular Componentnuclear outer membrane
Cellular Componentnucleus-vacuole junction
Cellular Componentphagophore assembly site
Molecular Functionidentical protein binding
Molecular Functionprotein-membrane adaptor activity
Biological Processautophagosome assembly
Biological Processautophagy
Biological ProcessCvt vesicle assembly
Biological Processestablishment of organelle localization
Biological Processlipid metabolic process
Biological Processmacroautophagy
Biological Processnucleus-vacuole junction assembly
Biological Processpexophagy
Biological Processpiecemeal microautophagy of the nucleus
Biological Processprotein localization to membrane raft
Biological Processprotein localization to phagophore assembly site
Biological Processprotein-containing complex localization
Biological Processribophagy
Biological Processvacuole fusion, non-autophagic
Biological Processvacuole inheritance

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Vacuolar protein 8

Gene names

    • Name
      VAC8
    • Synonyms
      YEB3
    • Ordered locus names
      YEL013W

Organism names

Accessions

  • Primary accession
    P39968
  • Secondary accessions
    • D3DLN6
    • Q549T0

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis4-7In VAC8-3; not palmitoylated.
Mutagenesis51Fails to undergo self-association in the presence of NVJ1 or ATG13.
Mutagenesis60Fails to support the Cvt pathway, but does not affect the PMN pathway; when associated with R-62.
Mutagenesis62Does not affect self-association in the presence of NVJ1 but fails to undergo self-association in the presence of ATG13. Fails to support the cvt pathway, but does not affect the PMN pathway; when associated with R-60.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, lipidation, chain, modified residue, cross-link.

TypeIDPosition(s)Description
Initiator methionine1Removed
Lipidation2N-myristoyl glycine
ChainPRO_00000643022-578Vacuolar protein 8
Lipidation4S-palmitoyl cysteine
Lipidation5S-palmitoyl cysteine
Lipidation7S-palmitoyl cysteine
Modified residue11Phosphoserine
Modified residue16Phosphoserine
Cross-link77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link515Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Post-translational modification

Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with NVJ1 (PubMed:10888680, PubMed:16912077, PubMed:28533415).
Forms heterotetramers of two VAC8 and two NVJ1 or two VAC8 and two ATG13 (PubMed:31512555).

Binary interactions

View interactors in UniProtKB
View CPX-1304 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for repeat, region, compositional bias.

TypeIDPosition(s)Description
Repeat37-75ARM 1
Repeat76-114ARM 2
Repeat116-155ARM 3
Repeat157-196ARM 4
Repeat198-237ARM 5
Repeat239-280ARM 6
Repeat282-321ARM 7
Repeat323-363ARM 8
Repeat407-446ARM 9
Region527-557Disordered
Compositional bias530-557Polar residues

Domain

Comprises a flexibly connected N-terminal H1 helix followed by armadillo repeats (ARMs) that form a right-handed superhelical structure.
the H1 helix directly interacts with ARM1 and hinders the dimeric interface (PubMed:28533415, PubMed:31512555).
ARM1 serves as a binding interface for the formation of the 2-fold dimer of VAC8, which is crucial for heterotetramer formation with either NVJ1 or ATG13 and subsequent PMN and Cvt pathways (PubMed:28533415, PubMed:31512555).
The highly conserved innergroove formed by the ARMs specifically binds the extended 80 Angstroms-longloop of NVJ1 (PubMed:28533415).
The ARMs domain also binds the he 70 Angstroms extended loop of ATG13 in an antiparallel manner (PubMed:31512555).

Sequence similarities

Belongs to the beta-catenin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    578
  • Mass (Da)
    63,208
  • Last updated
    2007-01-23 v3
  • Checksum
    B2E75774B47E5933
MGSCCSCLKDSSDEASVSPIADNEREAVTLLLGYLEDKDQLDFYSGGPLKALTTLVYSDNLNLQRSAALAFAEITEKYVRQVSREVLEPILILLQSQDPQIQVAACAALGNLAVNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGCITNLATRDDNKHKIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLESHNDKVEDLVKNDDDIINGVRKMADVTFERLQRSGIDVKNPGSNNNPSSNDNNSNNNDTGSEHQPVEDASLELYNITQQILQFLH

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias530-557Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF005267
EMBL· GenBank· DDBJ
AAQ13402.1
EMBL· GenBank· DDBJ
mRNA
U18530
EMBL· GenBank· DDBJ
AAB64490.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006939
EMBL· GenBank· DDBJ
DAA07640.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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