P39923 · NPR2_YEAST
- ProteinNitrogen permease regulator 2
- GeneNPR2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids615 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis (PubMed:21454883).
Mediates inactivation of the TORC1 complex in response to amino acid starvation (PubMed:19521502, PubMed:26510498).
Post-transcriptional regulator of nitrogen permeases (PubMed:19521502).
May be involved in putative NPR1-dependent phosphorylation of nitrogen permeases or in the processing and targeting of nitrogen permeases at the level of the endoplasmic reticulum (PubMed:19521502).
Mediates inactivation of the TORC1 complex in response to amino acid starvation (PubMed:19521502, PubMed:26510498).
Post-transcriptional regulator of nitrogen permeases (PubMed:19521502).
May be involved in putative NPR1-dependent phosphorylation of nitrogen permeases or in the processing and targeting of nitrogen permeases at the level of the endoplasmic reticulum (PubMed:19521502).
Miscellaneous
Present with 319 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | GATOR1 complex | |
Cellular Component | Seh1-associated complex | |
Cellular Component | vacuolar membrane | |
Biological Process | cellular response to amino acid starvation | |
Biological Process | cellular response to nitrogen starvation | |
Biological Process | negative regulation of TORC1 signaling | |
Biological Process | positive regulation of autophagy | |
Biological Process | proline transport | |
Biological Process | protein transport | |
Biological Process | regulation of autophagosome assembly | |
Biological Process | regulation of TORC1 signaling | |
Biological Process | response to xenobiotic stimulus | |
Biological Process | urea transport |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrogen permease regulator 2
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP39923
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Vacuole membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
TORC1 abnormally activated in presence of rapamycin.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 5 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000213321 | 1-615 | Nitrogen permease regulator 2 | |||
Sequence: MLSYFQGFVPIHTIFYSVFHPTEGSKIKYEFPPNNLKNHGINFNTFKNYIIPKPILCHKLITFKYGTYRIVCYPVTINSPIYARNFFSFNFVFVFPYDCETSPYEPAITRLGKMFKVLEEQNQLLSKSERDPVFFDLKVLENSTTTPSTAGPSSTPNPSSNTTPTHPTSEKDTKDMRSSRYSDLIKDLGLPQSAFSIQDLLMRIFQDLNNYSECLIPIDEGNAVDIKIFPLLRPPTTCVSLEDVPLSSVNLKKIIDVNWDPTMMSIVPYIDGLNSIAKISKLSNSDPGLVIECIRHLIYYKCVTLSDIFQFSNIYAPSSLIRNFLTDPLMASDCQSYVTFPEVSKISNLPLNKSLGSGDQDSPSFSVRRKSKSSSIPSNPDSRTTSFSSTSRVSQNSSLNSSFSSIYKDWRQSQTSCSSSNIHVINNRNRFLPTRSCLFDLYRSLSQGQTLKTWYESKYMILKENNIDIRRFITFGLEKRIIYRCYSFPVMINAGSREPKEMTPIITKDLVNNDKLLEKRNHNHLLSATGSRNTAQSGNLKPERPSKVSFEMQRVSSLATGKSTMPKLSDEEEGILEESIRNAETFDKICVLLSKPKLEVESYLNELGEFKVINS | ||||||
Modified residue | 362 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
By urea and proline.
Interaction
Subunit
Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1. Forms a heterodimer with NPR3.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P39923 | NPR3 P38742 | 5 | EBI-12212, EBI-24336 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 143-167 | Polar residues | ||||
Sequence: STTTPSTAGPSSTPNPSSNTTPTHP | ||||||
Region | 143-176 | Disordered | ||||
Sequence: STTTPSTAGPSSTPNPSSNTTPTHPTSEKDTKDM | ||||||
Region | 354-398 | Disordered | ||||
Sequence: SLGSGDQDSPSFSVRRKSKSSSIPSNPDSRTTSFSSTSRVSQNSS | ||||||
Compositional bias | 527-543 | Polar residues | ||||
Sequence: SATGSRNTAQSGNLKPE | ||||||
Region | 527-551 | Disordered | ||||
Sequence: SATGSRNTAQSGNLKPERPSKVSFE |
Sequence similarities
Belongs to the NPR2 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length615
- Mass (Da)69,859
- Last updated2005-12-20 v2
- Checksum58CA4983B0BAF7BA
Sequence caution
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 143-167 | Polar residues | ||||
Sequence: STTTPSTAGPSSTPNPSSNTTPTHP | ||||||
Sequence conflict | 225 | in Ref. 4; AAT92885 | ||||
Sequence: D → G | ||||||
Compositional bias | 527-543 | Polar residues | ||||
Sequence: SATGSRNTAQSGNLKPE |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X79105 EMBL· GenBank· DDBJ | CAA55721.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
U18795 EMBL· GenBank· DDBJ | AAB65025.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AY692866 EMBL· GenBank· DDBJ | AAT92885.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
BK006939 EMBL· GenBank· DDBJ | DAA07592.1 EMBL· GenBank· DDBJ | Genomic DNA |