P39914 · BRXC_BACSU
- ProteinMonothiol bacilliredoxin BrxC
- GenebrxC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids108 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
S-bacillithiolation is the formation of mixed disulfide bonds between protein thiols and the general thiol reductant bacillithiol (BSH) under oxidative stress. BSH is an equivalent of glutathione (GSH) in Firmicutes. This protein is a monothiol bacilliredoxin, which debacillithiolates (removes BSH) the S-bacillithiolated glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) GapA and GapB in vivo and probably a number of other oxidized cytosolic proteins. Debacillithiolates the S-bacillithiolated Bdr (Bdr-SSB) and BrxB (BrxB-SSB) in vitro. Involved in maintaining redox homeostasis in response to disulfide stress conditions.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Molecular Function | enzyme binding | |
Molecular Function | oxidoreductase activity | |
Molecular Function | translation elongation factor binding | |
Biological Process | cell redox homeostasis | |
Biological Process | response to oxidative stress |
Keywords
- Molecular function
Enzyme and pathway databases
Community annotation
Monothiol bacilliredoxin.
Source | Submission date | Contributor |
---|---|---|
PubMed:33722570 | 0000-0001-6837-2941 |
Names & Taxonomy
Protein names
- Recommended nameMonothiol bacilliredoxin BrxC
- Short namesMonothiol Brx-C
- Alternative names
Gene names
Community suggested name: YtxJ
Source | Submission date | Contributor |
---|---|---|
PubMed:33722570 | 0000-0001-6837-2941 |
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionP39914
- Secondary accessions
Proteomes
Phenotypes & Variants
Disruption phenotype
Cells lacking the operon including this gene (ytxGH-brxC) have increased S-bacillithiolation of glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) GapA and GapB in cells with increased basal levels of oxidative stress due to concomitant deletion of genes encoding for catalase (katA) and alkyl hydroperoxide reductase (ahpCF).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 31 | Loss of debacillithiolation of S-bacillithiolated Bdr (Bdr-SSB). | ||||
Sequence: C → A |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000049906 | 1-108 | Monothiol bacilliredoxin BrxC | |||
Sequence: MAKQLIQSEEEFKRIAEQEGVFVFLKHSTTCPISQAAFHEFDAFANQHEDVPAYYLQVQEARPLSNFIAETYGVKHESPQIFIIQNGEVKWHTSHSQITEAAIEQHLS | ||||||
Modified residue | 31 | S-bacillithiol cysteine disulfide | ||||
Sequence: C |
Post-translational modification
Cys can react with bacillithiol (BSH) to form mixed disulfides. S-bacillithiolation protects Cys residues against overoxidation by acting as a redox switch in response to oxidative stress.
Proteomic databases
Interaction
Subunit
Interacts with AbrB, BdhA, Bdr, BrxB, FolD, GapA, GapB, GatA, PfkA, PyrAA, PyrAB, PyrE, PyrG, PyrH, RpsB, RpsK, RpsL, SalA, SucC, Tuf and YtsJ.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length108
- Mass (Da)12,402
- Last updated1995-02-01 v1
- ChecksumD8F03DD68E79AF38
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X65945 EMBL· GenBank· DDBJ | CAA46762.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF008220 EMBL· GenBank· DDBJ | AAC00297.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB14954.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP052842 EMBL· GenBank· DDBJ | QJP89694.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
L31845 EMBL· GenBank· DDBJ | AAB40046.1 EMBL· GenBank· DDBJ | Genomic DNA |