P39793 · PBPA_BACSU

Function

function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (Probable). Required for vegetative growth (Probable). Has a partially redundant function with PBP-2A (pbpA) during spore outgrowth (PubMed:9851991).

Catalytic activity

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site115Proton donor; for transglycosylase activity
Active site390Acyl-ester intermediate; for transpeptidase activity

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionpenicillin binding
Molecular Functionpeptidoglycan glycosyltransferase activity
Molecular Functionserine-type D-Ala-D-Ala carboxypeptidase activity
Biological Processcell wall organization
Biological Processpeptidoglycan biosynthetic process
Biological Processproteolysis
Biological Processregulation of cell shape
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Penicillin-binding protein 1A/1B
  • Short names
    PBP1

Including 2 domains:

  • Recommended name
    Penicillin-insensitive transglycosylase
  • EC number
  • Alternative names
    • Peptidoglycan TGase
  • Recommended name
    Penicillin-sensitive transpeptidase
  • EC number
  • Alternative names
    • DD-transpeptidase

Gene names

    • Name
      ponA
    • Ordered locus names
      BSU22320

Organism names

  • Taxonomic identifier
  • Strains
    • 168
    • 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / NRRL NRS-744 / VKM B-501
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus

Accessions

  • Primary accession
    P39793

Proteomes

Subcellular Location

Cell membrane
; Single-pass type II membrane protein
Forespore inner membrane
; Single-pass type II membrane protein
Note: Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-37Cytoplasmic
Transmembrane38-58Helical; Signal-anchor for type II membrane protein
Topological domain59-914Extracellular

Keywords

Phenotypes & Variants

Disruption phenotype

Cells require increased levels of Mg2+ or Ca2+ for growth and germination. Approximately 50% of cells without the gene contain abnormal FtsZ rings, suggesting it is involved in septum synthesis; increased levels of Mg2+ or Ca2+ only partially eliminate the septation defects (PubMed:9721295).
Double ponA-pbpA deletions spores have greatly decreased viability, peptidoglycan synthesis and elongate poorly; increased levels of Mg2+ increase spore viability (PubMed:9851991).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000831791-914Penicillin-binding protein 1A/1B

Post-translational modification

The product expressed from the translation of the ponA gene appears as two bands on a gel (1A and 1B), but the specific amino acid sequence of each protein is unknown.
The N-terminus is blocked.

Proteomic databases

Expression

Developmental stage

Expression is constant during growth, decreases during sporulation and is induced approximately 15 minutes into spore germination. Present in the inner forespore membrane of the dormant spore (PubMed:3080407).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-16Basic and acidic residues
Region1-29Disordered
Region77-246Transglycosylase
Region329-662Transpeptidase
Domain708-795Fibronectin type-III
Region773-914Disordered
Compositional bias789-844Basic and acidic residues
Compositional bias845-914Polar residues

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 51 family.
In the C-terminal section; belongs to the transpeptidase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    914
  • Mass (Da)
    99,562
  • Last updated
    1995-02-01 v1
  • Checksum
    6978E33DFE2423E6
MSDQFNSREARRKANSKSSPSPKKGKKRKKGGLFKKTLFTLLILFVLGVVGGAVTFAVMVSDAPSLDESKLKTPYSSTIYDKNGKEIAEVGAEKRTYVSIDEIPDVVKEAFIATEDARFYEHHGIDPVRIGGALVANFKDGFGAEGGSTITQQVVKNSLLSHQKTLKRKVQEVWLSIQLERNYSKDEILEMYLNRIYFSPRAYGIGKAAEEFFGVTDLSKLTVEQAATLAGMPQSPTAYNPVKNPDKAEKRRNIVLSLMKKQGFISDSQYNKAKKVAVKDEGVVSQKEYEKASTNKYSAFVEEVMKEIDEKSDVDPSADGLKIYTTLDTKAQDKLDELMDGDTVGFTEGMQGGVTLLDTKNGEVRAIGAGRNQPVGGFNYATQTKAQPGSTIKPILDYGPVIENKKWSTYEQIDDSAYTYSNGKPIRDWDRKYLGPISMRYALAQSRNIPALKAFQAVGKDTAVDFANGLGLGLTKDNVTEAYSIGGFGGNDGVSPLTMAGAYSAFGNNGTYNEPHFVKSIEFNDGTKLDLTPKSKSAMSDYTAFMITDMLKTAVKTGTGQLAQVPGVEVAGKTGTTNFDDNEVKRYNIASGGARDSWFVGYTPQYTAAVWTGMGENEAGKKSLSAEEQKVAKRIFAQLIADVDDGSGSFEKPDSVVEATVEKGSNPAKLAGPNTPSDKKLTEYFVKGTAPSTVSKTYEKEEKEETAKLSGLNVKYDKDNQSLTLSWNYDGDATFAVKQSVDGGSYSEIQNSSAKEAVISGVQPGSVYKFEVTAVSDDGKSTASTSYEVPKAEDDEDKKDQQQTDDEKQDDEKTQDDTQTDDSQKDDGQTDQDQTDDSTNDQDKKQDNTNTNPSDNNNQDQSNDNDNDNSNNQDTSDGDSNSGKNDSTGSDTNKNKTDTSNKTQTNSSSIEKTN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-16Basic and acidic residues
Compositional bias789-844Basic and acidic residues
Compositional bias845-914Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U11883
EMBL· GenBank· DDBJ
AAA64947.1
EMBL· GenBank· DDBJ
Genomic DNA
L47838
EMBL· GenBank· DDBJ
AAB38459.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB14148.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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