P39771 · PURT_BACSU

  • Protein
    Formate-dependent phosphoribosylglycinamide formyltransferase
  • Gene
    purT
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP.
Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide from N1-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site14-15N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site74N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site106ATP (UniProtKB | ChEBI)
Binding site147ATP (UniProtKB | ChEBI)
Binding site152-157ATP (UniProtKB | ChEBI)
Binding site187-190ATP (UniProtKB | ChEBI)
Binding site195ATP (UniProtKB | ChEBI)
Binding site259Mg2+ (UniProtKB | ChEBI)
Binding site271Mg2+ (UniProtKB | ChEBI)
Binding site278N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site348N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)
Binding site355-356N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionligase activity
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylglycinamide formyltransferase 2 activity
Molecular Functionphosphoribosylglycinamide formyltransferase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Formate-dependent phosphoribosylglycinamide formyltransferase
  • EC number
  • Alternative names
    • 5'-phosphoribosylglycinamide transformylase 2
    • Formate-dependent GAR transformylase
    • GAR transformylase 2
      (GART 2
      )
    • Non-folate glycinamide ribonucleotide transformylase
    • Phosphoribosylglycinamide formyltransferase 2

Gene names

    • Name
      purT
    • Ordered locus names
      BSU02230

Organism names

Accessions

  • Primary accession
    P39771
  • Secondary accessions
    • O31450

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000749551-384Formate-dependent phosphoribosylglycinamide formyltransferase

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain111-300ATP-grasp

Sequence similarities

Belongs to the PurK/PurT family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    384
  • Mass (Da)
    42,093
  • Last updated
    1998-12-15 v2
  • Checksum
    5E3642CD6CAF90F7
MYQSKKVLLLGSGELGKEVVIEAQRLGVQTVAVDSYEHAPAMQVAHNSYVVDMLDPEQIRTIIEKENPDLIVPEVEAIATDELLKLEEEGFHVIPNARAAKLTMDREGIRRLAAETLGLATAGYEFANTYDEFIQAAAQIGFPCVVKPLMSSSGKGQSVCRSEADLESCWETAMEGGRVKNGRVIVEEFIPFESEITLLTVRAVNGTAFCEPIGHVQKDGDYIESWQPHDMTEQQIEEAKHIAKTITDELGGYGLFGVELFLAKDRVYFSEVSPRPHDTGLVTLVTQNLSEFALHVRAILGFPITEITQLSPGASRPLKAPEELADYTVEGLENALAVPKTQVRVFGKPITKAGRRMAVALSAADSVETARENAKKALDQLILK

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict240-241in Ref. 1; CAA55557

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X78962
EMBL· GenBank· DDBJ
CAA55557.1
EMBL· GenBank· DDBJ
Genomic DNA
AB006424
EMBL· GenBank· DDBJ
BAA33120.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB12017.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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