P39724 · BOL3_YEAST

Function

function

Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates [4Fe-4S] cluster insertion into a subset of mitochondrial proteins such as lipoyl synthase (LS) and succinate dehydrogenase (SDH) (PubMed:27532772, PubMed:27532773).
Required during the last step of iron-sulfur protein assembly when the iron-sulfur cluster is inserted into the target protein (PubMed:27532772).
Acts together with NFU1, later than BOL1 and GRX5 in the [4Fe-4S] cluster insertion process (PubMed:27532773).
Not required for [2Fe-2S] cluster insertion into mitochondrial proteins (PubMed:27532772).

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentiron-sulfur cluster assembly complex
Cellular Componentmitochondrial matrix
Cellular Componentmitochondrion
Biological Processintracellular iron ion homeostasis
Biological Processiron-sulfur cluster assembly
Biological Processprotein maturation by [4Fe-4S] cluster transfer

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    BolA-like protein 3
  • Alternative names
    • Altered inheritance of mitochondria protein 1

Gene names

    • Name
      BOL3
    • Synonyms
      AIM1
    • Ordered locus names
      YAL046C

Organism names

Accessions

  • Primary accession
    P39724
  • Secondary accessions
    • D6VPH0

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Increases frequency of mitochondrial genome loss (PubMed:19300474).
Cells show a slight decrease of succinate dehydrogenase (SDH) (PubMed:27532772).
Cells lacking BOL1 and BOL3 display defects in a subset of mitochondrial [4Fe-4S] enzymes (PubMed:27532772, PubMed:27532773).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis64Partial loss of function.
Mutagenesis101Loss of function.
Mutagenesis101Dominant negative mutant; displays respiratory defects that are higher than the BOL1 BOL3 double mutant.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002012391-118BolA-like protein 3

Proteomic databases

Interaction

Subunit

Interacts with NFU1 (PubMed:27532773).
View interactors in UniProtKB
View CPX-6930 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Belongs to the BolA/IbaG family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    118
  • Mass (Da)
    13,356
  • Last updated
    1995-02-01 v1
  • Checksum
    244F5FF2052FF410
MKLPQTMLRSISVKHVRWPRILTGSKLWYSTQMAMTPEEKMITDKLQQELEPEVCKVQDVSGGCGSMFAINITSKKFNGLSLIKQHQLVNRILRDDISRWHGLQLTTKKSTGKGPASS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U12980
EMBL· GenBank· DDBJ
AAC04985.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006935
EMBL· GenBank· DDBJ
DAA06940.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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