P39371 · NANM_ECOLI

Function

function

Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses (PubMed:18063573).
Forms linear aceneuramate during interconversion of Neu5Ac anomers (PubMed:33895133).

Catalytic activity

Features

Showing features for active site.

136850100150200250300350
TypeIDPosition(s)Description
Active site228Proton acceptor

GO annotations

AspectTerm
Cellular Componentouter membrane-bounded periplasmic space
Molecular Functionprotein homodimerization activity
Molecular Functionracemase and epimerase activity, acting on carbohydrates and derivatives
Biological ProcessN-acetylneuraminate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    N-acetylneuraminate epimerase
  • EC number
  • Alternative names
    • N-acetylneuraminate anomerase NanM
    • N-acetylneuraminate mutarotase (Neu5Ac mutarotase)
    • Sialic acid epimerase

Gene names

    • Name
      nanM
    • Synonyms
      yjhT
    • Ordered locus names
      b4310, JW5777

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P39371
  • Secondary accessions
    • Q2M603

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis30No effect on catalytic activity.
Mutagenesis228Great decrease in catalytic activity.
Mutagenesis234Decrease in catalytic activity.
Mutagenesis297No effect on catalytic activity.
Mutagenesis302No effect on catalytic activity.
Mutagenesis328No effect on catalytic activity.
Mutagenesis344No effect on catalytic activity.

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-19
ChainPRO_000001665520-368N-acetylneuraminate epimerase

Proteomic databases

Expression

Induction

Induced by N-acetylneuraminate and modulated by N-acetylglucosamine, via the NanR and NagC regulators.

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for repeat.

TypeIDPosition(s)Description
Repeat40-84Kelch 1
Repeat86-137Kelch 2
Repeat139-173Kelch 3
Repeat174-219Kelch 4
Repeat222-265Kelch 5
Repeat287-336Kelch 6
Repeat338-367Kelch 7

Sequence similarities

Belongs to the NanM family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    368
  • Mass (Da)
    39,572
  • Last updated
    2000-05-30 v2
  • Checksum
    1194F392C51EA204
MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIGLGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQVFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNEAGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLITVKDNKVTVQN

Sequence caution

The sequence AAA97206.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Mass Spectrometry

Molecular mass is 38,685 Da. Determined by Electrospray.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U14003
EMBL· GenBank· DDBJ
AAA97206.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
U00096
EMBL· GenBank· DDBJ
AAC77266.2
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE78303.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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