P39001 · UME6_YEAST

Function

function

Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Binds to the URS1 site (5'-AGCCGCCGA-3') and recruits the RPD3 histone deacetylase complex to the promoters to negatively regulate the expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. Recruits also the ISW2 chromatin remodeling complex to promoters in a second gene repression pathway. Associates with the master regulator of meiosis IME1 in order to activate the expression of meiosis genes. Has both a positive and negative role in regulating phospholipid biosynthesis.

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.

Features

Showing features for dna binding.

1836100200300400500600700800
TypeIDPosition(s)Description
DNA binding771-798Zn2-C6 fungal-type

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular ComponentRpd3L complex
Cellular ComponentRpd3L-Expanded complex
Cellular Componenttranscription regulator complex
Molecular FunctionDNA-binding transcription factor binding
Molecular FunctionDNA-binding transcription repressor activity, RNA polymerase II-specific
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Functionsequence-specific DNA binding
Molecular Functionzinc ion binding
Biological Processcellular response to nitrogen starvation
Biological Processchromatin remodeling
Biological Processlipid droplet organization
Biological Processmeiotic cell cycle
Biological Processmitotic cell cycle
Biological Processnegative regulation of inositol biosynthetic process
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processnitrogen catabolite repression of transcription from RNA polymerase II promoter
Biological Processpositive regulation of phosphatidylcholine biosynthetic process
Biological Processpositive regulation of phosphatidylserine biosynthetic process
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processpseudohyphal growth
Biological Processregulation of sporulation resulting in formation of a cellular spore
Biological Processspore germination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transcriptional regulatory protein UME6
  • Alternative names
    • Negative transcriptional regulator of IME2
    • Regulator of inducer of meiosis protein 16
    • Unscheduled meiotic gene expression protein 6

Gene names

    • Name
      UME6
    • Synonyms
      CAR80, CARGR1, NIM2, RIM16
    • ORF names
      YD8142.04C
    • Ordered locus names
      YDR207C

Organism names

Accessions

  • Primary accession
    P39001
  • Secondary accessions
    • D6VSI8

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis99Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation.
Mutagenesis99-107Impairs meiotic genes expression and sporulation, reduces the interaction with IME1, and abolishes phosphorylation.
Mutagenesis99-109Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation.
Mutagenesis103Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation.
Mutagenesis107Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation.
Mutagenesis523Impairs SIN3-binding and gene repression activity.
Mutagenesis524Impairs gene repression activity.
Mutagenesis525Impairs gene repression activity.
Mutagenesis526Impairs gene repression activity.
Mutagenesis527Impairs SIN3-binding and gene repression activity.
Mutagenesis528Impairs SIN3-binding and gene repression activity.
Mutagenesis530Impairs SIN3-binding and gene repression activity.
Mutagenesis635Impairs gene repression activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 18 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001149871-836Transcriptional regulatory protein UME6
Modified residue114Phosphoserine
Modified residue141Phosphoserine
Modified residue150Phosphoserine
Modified residue228Phosphoserine
Modified residue316Phosphoserine
Modified residue318Phosphoserine
Modified residue645Phosphoserine

Post-translational modification

Phosphorylated by RIM11 and MCK1.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Interacts with RIM11, MCK1 and IME1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P39001IME1 P211903EBI-20086, EBI-9199
View interactors in UniProtKB
View CPX-1415 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-77Disordered
Compositional bias12-48Polar residues
Compositional bias63-77Polar residues
Compositional bias92-112Polar residues
Region92-168Disordered
Compositional bias150-168Polar residues
Region218-332Disordered
Compositional bias228-305Polar residues
Compositional bias314-328Polar residues
Region381-464Disordered
Region508-594SIN3-binding
Region636-766Disordered
Compositional bias666-699Polar residues
Compositional bias736-766Polar residues

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    836
  • Mass (Da)
    91,124
  • Last updated
    1996-10-01 v2
  • Checksum
    0DDA0A6B4A157182
MLDKARSQSKHMDESNAAASLLSMETTANNHHYLHNKTSRATLMNSSQDGKKHAEDEVSDGANSRHPTISSASIESLKTTYDENPLLSIMKSTCAPNNTPVHTPSGSPSLKVQSGGDIKDDPKENDTTTTTNTTLQDRRDSDNAVHAAASPLAPSNTPSDPKSLCNGHVAQATDPQISGAIQPQYTATNEDVFPYSSTSTNSNTATTTIVAGAKKKIHLPPPQAPAVSSPGTTAAGSGAGTGSGIRSRTGSDLPLIITSANKNNGKTTNSPMSILSRNNSTNNNDNNSIQSSDSRESSNNNEIGGYLRGGTKRGGSPSNDSQVQHNVHDDQCAVGVAPRNFYFNKDREITDPNVKLDENESKINISFWLNSKYRDEAYSLNESSSNNASSNTDTPTNSRHANTSSSITSRNNFQHFRFNQIPSQPPTSASSFTSTNNNNPQRNNINRGEDPFATSSRPSTGFFYGDLPNRNNRNSPFHTNEQYIPPPPPKYINSKLDGLRSRLLLGPNSASSSTKLDDDLGTAAAVLSNMRSSPYRTHDKPISNVNDMNNTNALGVPASRPHSSSFPSKGVLRPILLRIHNSEQQPIFESNNSTAVFDEDQDQNQDLSPYHLNLNSKKVLDPTFESRTRQVTWNKNGKRIDRRLSAPEQQQQLEVPPLKKSRRSVGNARVASQTNSDYNSLGESSTSSAPSSPSLKASSGLAYTADYPNATSPDFAKSKGKNVKPKAKSKAKQSSKKRPNNTTSKSKANNSQESNNATSSTSQGTRSRTGCWICRLRKKKCTEERPHCFNCERLKLDCHYDAFKPDFVSDPKKKQMKLEEIKKKTKEAKRRAMKKK

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias12-48Polar residues
Compositional bias63-77Polar residues
Compositional bias92-112Polar residues
Sequence conflict101in Ref. 3; BAA04890
Compositional bias150-168Polar residues
Compositional bias228-305Polar residues
Compositional bias314-328Polar residues
Sequence conflict363in Ref. 1; AAA34471
Sequence conflict443in Ref. 1; AAA34471
Sequence conflict465in Ref. 1; AAA34471
Compositional bias666-699Polar residues
Compositional bias736-766Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L32186
EMBL· GenBank· DDBJ
AAA34471.1
EMBL· GenBank· DDBJ
Genomic DNA
L24539
EMBL· GenBank· DDBJ
AAC14472.1
EMBL· GenBank· DDBJ
Unassigned DNA
D23663
EMBL· GenBank· DDBJ
BAA04890.1
EMBL· GenBank· DDBJ
Genomic DNA
Z68194
EMBL· GenBank· DDBJ
CAA92346.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006938
EMBL· GenBank· DDBJ
DAA12048.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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