P38862 · ATG7_YEAST
- ProteinUbiquitin-like modifier-activating enzyme ATG7
- GeneATG7
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids630 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Plays a role in the regulation of filamentous growth and chronological longevity.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 507 | Glycyl thioester intermediate | ||||
Sequence: C |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | extrinsic component of phagophore assembly site membrane | |
Cellular Component | membrane | |
Cellular Component | mitochondrion | |
Cellular Component | phagophore assembly site | |
Molecular Function | Atg12 activating enzyme activity | |
Molecular Function | Atg8 activating enzyme activity | |
Molecular Function | identical protein binding | |
Biological Process | autophagy | |
Biological Process | autophagy of mitochondrion | |
Biological Process | cellular response to nitrogen starvation | |
Biological Process | cytoplasm to vacuole targeting by the Cvt pathway | |
Biological Process | macroautophagy | |
Biological Process | nucleophagy | |
Biological Process | piecemeal microautophagy of the nucleus | |
Biological Process | protein modification by small protein conjugation |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUbiquitin-like modifier-activating enzyme ATG7
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP38862
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 333 | Loss of interaction with ATG8 and ATG12, and no more ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy. | ||||
Sequence: G → A | ||||||
Mutagenesis | 443 | Loss of interaction with ATG8. | ||||
Sequence: R → A | ||||||
Mutagenesis | 466 | Loss of interaction with ATG8; when associated with F-486 and A-490. | ||||
Sequence: S → A | ||||||
Mutagenesis | 486 | Loss of interaction with ATG8; when associated with A-466 and A-490. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 490 | Loss of interaction with ATG8; when associated with A-466 and F-486. | ||||
Sequence: D → A | ||||||
Mutagenesis | 507 | Loss of interaction with ATG8 and ATG12 and no more formation of ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy. | ||||
Sequence: C → A | ||||||
Mutagenesis | 507 | Instead of the formation of an intermediate complex with a thiol ester bond between ATG7 (E1-like enzyme) and ATG8 (substrate) or between ATG7 and ATG12 (substrate), a stable complex with an O-ester bond is formed. No more formation of ATG12-ATG5 conjugate. | ||||
Sequence: C → S | ||||||
Mutagenesis | 511 | Impaired homodimerization and ATP-binding. Homodimerization and ATP-binding are recovered when it heterodimerizes with an ATG7 molecule with a R-524 mutation. | ||||
Sequence: R → A | ||||||
Mutagenesis | 524 | Impaired homodimerization and ATP-binding. Homodimerization and ATP-binding are recovered when it heterodimerizes with an ATG7 molecule with a A-511 mutation. | ||||
Sequence: E → R | ||||||
Mutagenesis | 550 | Loss of interaction with ATG8. | ||||
Sequence: R → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 11 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000212822 | 1-630 | Ubiquitin-like modifier-activating enzyme ATG7 | |||
Sequence: MSSERVLSYAPAFKSFLDTSFFQELSRLKLDVLKLDSTCQPLTVNLDLHNIPKSADQVPLFLTNRSFEKHNNKRTNEVPLQGSIFNFNVLDEFKNLDKQLFLHQRALECWEDGIKDINKCVSFVIISFADLKKYRFYYWLGVPCFQRPSSTVLHVRPEPSLKGLFSKCQKWFDVNYSKWVCILDADDEIVNYDKCIIRKTKVLAIRDTSTMENVPSALTKNFLSVLQYDVPDLIDFKLLIIRQNEGSFALNATFASIDPQSSSSNPDMKVSGWERNVQGKLAPRVVDLSSLLDPLKIADQSVDLNLKLMKWRILPDLNLDIIKNTKVLLLGAGTLGCYVSRALIAWGVRKITFVDNGTVSYSNPVRQALYNFEDCGKPKAELAAASLKRIFPLMDATGVKLSIPMIGHKLVNEEAQHKDFDRLRALIKEHDIIFLLVDSRESRWLPSLLSNIENKTVINAALGFDSYLVMRHGNRDEQSSKQLGCYFCHDVVAPTDSLTDRTLDQMCTVTRPGVAMMASSLAVELMTSLLQTKYSGSETTVLGDIPHQIRGFLHNFSILKLETPAYEHCPACSPKVIEAFTDLGWEFVKKALEHPLYLEEISGLSVIKQEVERLGNDVFEWEDDESDEIA |
Proteomic databases
PTM databases
Interaction
Subunit
Homodimer; homodimerization is required for ATP-binding (PubMed:11139573, PubMed:21193819, PubMed:29295865).
Interacts with ATG8 through a thioester bond between Cys-507 and the C-terminal 'Gly-116' of ATG8 and with ATG12 through a thioester bond between Cys-507 and the C-terminal 'Gly-186' of ATG12 (PubMed:10233150, PubMed:11100732, PubMed:16874032, PubMed:18544538, PubMed:22055191).
Interacts also with ATG3 (PubMed:11139573, PubMed:17227760, PubMed:22056771, PubMed:23142976).
Interacts with ATG8 through a thioester bond between Cys-507 and the C-terminal 'Gly-116' of ATG8 and with ATG12 through a thioester bond between Cys-507 and the C-terminal 'Gly-186' of ATG12 (PubMed:10233150, PubMed:11100732, PubMed:16874032, PubMed:18544538, PubMed:22055191).
Interacts also with ATG3 (PubMed:11139573, PubMed:17227760, PubMed:22056771, PubMed:23142976).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P38862 | ATG10 Q07879 | 7 | EBI-2677, EBI-36629 | |
BINARY | P38862 | ATG3 P40344 | 10 | EBI-2677, EBI-3381 | |
BINARY | P38862 | ATG7 P38862 | 3 | EBI-2677, EBI-2677 | |
BINARY | P38862 | ATG8 P38182 | 12 | EBI-2677, EBI-2684 | |
XENO | P38862 | MAP1LC3B Q9GZQ8 | 2 | EBI-2677, EBI-373144 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 331-336 | GXGXXG motif | ||||
Sequence: GAGTLG | ||||||
Region | 591-630 | Homodimerization | ||||
Sequence: ALEHPLYLEEISGLSVIKQEVERLGNDVFEWEDDESDEIA |
Domain
The C-terminal 40 residues are required for homodimerization, as well as the interactions with ATG3, ATG8 and ATG12; and the C-terminal 17 residues are required for the ATG8 lipidation.
The GxGxxG motif is important for the function, possibly through binding with ATP.
Sequence similarities
Belongs to the ATG7 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length630
- Mass (Da)71,428
- Last updated1995-02-01 v1
- Checksum561EEC94F0ED57F5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB017925 EMBL· GenBank· DDBJ | BAA33474.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00027 EMBL· GenBank· DDBJ | AAB68016.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006934 EMBL· GenBank· DDBJ | DAA06864.1 EMBL· GenBank· DDBJ | Genomic DNA |