P38815 · YPT35_YEAST
- ProteinEndosomal/vacuolar adapter protein YPT35
- GeneYPT35
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids214 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Recruits the lipid transfer protein VPS13 to endosomal and vacuolar membranes.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endosome | |
Cellular Component | endosome membrane | |
Cellular Component | nucleus-vacuole junction | |
Cellular Component | vacuolar membrane | |
Molecular Function | phosphatidylinositol-3-phosphate binding | |
Molecular Function | protein-macromolecule adaptor activity | |
Biological Process | protein localization to endosome | |
Biological Process | protein localization to membrane |
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndosomal/vacuolar adapter protein YPT35
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP38815
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endosome membrane ; Peripheral membrane protein
Vacuole membrane ; Peripheral membrane protein
Note: Localizes to nuclear envelope-vacuole contact sites during respiration.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Decreases VPS13 levels at endosomal membranes and nuclear envelope-vacuole contact sites.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 5 | Disrupts interaction with VPS13. | ||||
Sequence: I → A | ||||||
Mutagenesis | 5-15 | Disrupts interaction with VPS13. | ||||
Sequence: Missing | ||||||
Mutagenesis | 8 | Disrupts interaction with VPS13. | ||||
Sequence: L → A | ||||||
Mutagenesis | 10 | Disrupts interaction with VPS13. | ||||
Sequence: P → A | ||||||
Mutagenesis | 12 | Disrupts interaction with VPS13. | ||||
Sequence: P → A | ||||||
Mutagenesis | 13 | Disrupts interaction with VPS13. | ||||
Sequence: I → A | ||||||
Mutagenesis | 15 | Disrupts interaction with VPS13. | ||||
Sequence: L → A | ||||||
Mutagenesis | 123 | Abolishes partially PtdIns3P-binding. | ||||
Sequence: Y → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000202910 | 1-214 | Endosomal/vacuolar adapter protein YPT35 | |||
Sequence: MSDKISFLPPEPIQLLDEDSTEPELDIDSQQENEGPISASNSNDSTSHSNDCGATITRTRPRRSSSINANFSFQKAHVSDCTIVNGDHGTKFAVWRITVFLEPNLKAFAAKRESYKIQTYKRYSDFVRLRENLLTRIKTAKPEKLNCLQIPHLPPSVQWYSSWKYQEVNLNKDWLAKRQRGLEYFLNHIILNSSLVEMTKDILIQFLEPSKRVA | ||||||
Modified residue | 65 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 66 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts (via PxP motif) with VPS13 (via SHR-BD domain) (PubMed:30018089).
Interacts with RBD2 (PubMed:15263065).
Interacts with YIF1 (PubMed:15263065).
Interacts with YIP1 (PubMed:15263065).
Interacts with YIP4 (PubMed:15263065).
Interacts with RBD2 (PubMed:15263065).
Interacts with YIF1 (PubMed:15263065).
Interacts with YIP1 (PubMed:15263065).
Interacts with YIP4 (PubMed:15263065).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P38815 | YIF1 P53845 | 4 | EBI-24665, EBI-28230 | |
BINARY | P38815 | YIP1 P53039 | 3 | EBI-24665, EBI-25295 | |
BINARY | P38815 | YIP4 P53093 | 2 | EBI-24665, EBI-24124 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, motif, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-63 | Disordered | ||||
Sequence: MSDKISFLPPEPIQLLDEDSTEPELDIDSQQENEGPISASNSNDSTSHSNDCGATITRTRPRR | ||||||
Motif | 5-15 | PxP | ||||
Sequence: ISFLPPEPIQL | ||||||
Compositional bias | 31-63 | Polar residues | ||||
Sequence: QENEGPISASNSNDSTSHSNDCGATITRTRPRR | ||||||
Domain | 73-213 | PX | ||||
Sequence: FQKAHVSDCTIVNGDHGTKFAVWRITVFLEPNLKAFAAKRESYKIQTYKRYSDFVRLRENLLTRIKTAKPEKLNCLQIPHLPPSVQWYSSWKYQEVNLNKDWLAKRQRGLEYFLNHIILNSSLVEMTKDILIQFLEPSKRV |
Domain
The PX domain binds phosphatidylinositol 3-phosphate (PtdIns3P) which is necessary for peripheral membrane localization.
Sequence similarities
Belongs to the YPT35 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length214
- Mass (Da)24,646
- Last updated1995-02-01 v1
- Checksum2ADD21AF107094DC
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 31-63 | Polar residues | ||||
Sequence: QENEGPISASNSNDSTSHSNDCGATITRTRPRR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U00059 EMBL· GenBank· DDBJ | AAB68857.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY557854 EMBL· GenBank· DDBJ | AAS56180.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006934 EMBL· GenBank· DDBJ | DAA06799.1 EMBL· GenBank· DDBJ | Genomic DNA |