P38715 · GRE3_YEAST

Function

function

Aldose reductase with a broad substrate specificity. Reduces the cytotoxic compound methylglyoxal (MG) to acetol and (R)-lactaldehyde under stress conditions. MG is synthesized via a bypath of glycolysis from dihydroxyacetone phosphate and is believed to play a role in cell cycle regulation and stress adaptation (PubMed:11525399).
In pentose-fermenting yeasts, aldose reductase catalyzes the reduction of xylose into xylitol. The purified enzyme catalyzes this reaction, but the inability of S.cerevisiae to grow on xylose as sole carbon source indicates that the physiological function is more likely methylglyoxal reduction (Probable) (PubMed:11722921).

Miscellaneous

Present with 12851 molecules/cell in log phase SD medium.
'De respuesta a estres' means stress response in Spanish.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.046 mMp-nitrobenzaldehyde
1.44 mMD-L-glyceraldehyde
1.57 mMD-glyceraldehyde
6.38 mML-glyceraldehyde
27.9 mMD-xylose
32.63 mML-arabinose
9.34 mMD-glucose
0.013 mMNADPH

pH Dependence

Optimum pH is 5.

Features

Showing features for active site, site, binding site.

TypeIDPosition(s)Description
Active site49Proton donor
Site78Lowers pKa of active site Tyr
Binding site111substrate
Binding site219-286NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functionaldose reductase (NADPH) activity
Molecular FunctionD-xylose reductase (NADPH) activity
Molecular FunctionmRNA binding
Biological Processarabinose catabolic process
Biological Processcellular response to osmotic stress
Biological Processcellular response to oxidative stress
Biological ProcessD-xylose catabolic process
Biological Processgalactose catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent aldose reductase GRE3
  • EC number
  • Short names
    AR
  • Alternative names
    • Genes de respuesta a estres protein 3
    • NADPH-dependent aldo-keto reductase GRE3
    • Xylose reductase
      (EC:1.1.1.-) . EC:1.1.1.- (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      GRE3
    • Ordered locus names
      YHR104W

Organism names

Accessions

  • Primary accession
    P38715
  • Secondary accessions
    • D3DL54

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00001246782-327NADPH-dependent aldose reductase GRE3

Proteomic databases

PTM databases

Expression

Induction

By osmotic, ionic, oxidative and heat stress.

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Belongs to the aldo/keto reductase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    327
  • Mass (Da)
    37,119
  • Last updated
    1995-02-01 v1
  • Checksum
    AFE72B8E2DFB91C8
MSSLVTLNNGLKMPLVGLGCWKIDKKVCANQIYEAIKLGYRLFDGACDYGNEKEVGEGIRKAISEGLVSRKDIFVVSKLWNNFHHPDHVKLALKKTLSDMGLDYLDLYYIHFPIAFKYVPFEEKYPPGFYTGADDEKKGHITEAHVPIIDTYRALEECVDEGLIKSIGVSNFQGSLIQDLLRGCRIKPVALQIEHHPYLTQEHLVEFCKLHDIQVVAYSSFGPQSFIEMDLQLAKTTPTLFENDVIKKVSQNHPGSTTSQVLLRWATQRGIAVIPKSSKKERLLGNLEIEKKFTLTEQELKDISALNANIRFNDPWTWLDGKFPTFA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U00059
EMBL· GenBank· DDBJ
AAB68858.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006934
EMBL· GenBank· DDBJ
DAA06798.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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