P38500 · NIR_BETPN
- ProteinFerredoxin--nitrite reductase, chloroplastic
- GeneNIR1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids583 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
Catalytic activity
- 6 oxidized [2Fe-2S]-[ferredoxin] + NH4+ + 2 H2O = nitrite + 6 reduced [2Fe-2S]-[ferredoxin] + 8 H+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 siroheme per subunit.
Note: Binds 1 [4Fe-4S] cluster per subunit.
Pathway
Nitrogen metabolism; nitrate reduction (assimilation).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 461 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 467 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 502 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 506 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 506 | Fe (UniProtKB | ChEBI) of siroheme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ferredoxin-nitrite reductase activity | |
Molecular Function | heme binding | |
Molecular Function | metal ion binding | |
Biological Process | nitrate assimilation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFerredoxin--nitrite reductase, chloroplastic
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fagales > Betulaceae > Betula
Accessions
- Primary accessionP38500
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-22 | Chloroplast | |||
Chain | PRO_0000019704 | 23-583 | Ferredoxin--nitrite reductase, chloroplastic | ||
Expression
Induction
By nitrate.
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length583
- Mass (Da)65,229
- Last updated1994-10-01 v1
- Checksum482111EF7E66991F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X60093 EMBL· GenBank· DDBJ | CAA42690.1 EMBL· GenBank· DDBJ | mRNA |