P38344 · REI1_YEAST
- ProteinCytoplasmic 60S subunit biogenesis factor REI1
- GeneREI1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids393 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Pre-60S-associated factor involved in the cytoplasmic maturation of the 60S subunit. Involved in the dissociation and recycling of other late pre-60S factors like ARX1, TIF6 and ALB1 before newly synthesized large ribosomal subunits enter translation. Cooperates with the co-chaperone JJJ1 in the release of the nuclear-export factor ARX1. May act redundantly with REH1 to directly promote a stabilizing structural rearrangement in cytoplasmic 60S subunit maturation independent on ARX1 recycling.
Miscellaneous
Present with 830 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | preribosome, large subunit precursor | |
Molecular Function | sequence-specific DNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | budding cell bud growth | |
Biological Process | mitotic cell cycle | |
Biological Process | nucleocytoplasmic transport | |
Biological Process | ribosomal large subunit biogenesis |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytoplasmic 60S subunit biogenesis factor REI1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP38344
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000202529 | 1-393 | Cytoplasmic 60S subunit biogenesis factor REI1 | |||
Sequence: MSSSGVYTCNSCVLTFDSSDEQRAHMKSDWHRYNLKRRVAQLPPISFETFDSKVSAAAASTSKSAEKEKPVTKKELKRREKQALLEKKKKLLEIARANMLENMQKSQEGNTPDLSKLSLQENEENKEKEEPKKEEPEQLTEEEMAERVMQEKLRNRVDIPLEQCLFCEHNKHFKDVEENLEHMFRTHGFYIPEQKYLVDKIGLVKYMSEKIGLGNICIVCNYQGRTLTAVRQHMLAKRHCKIPYESEDERLEISEFYDFTSSYANFNSNTTPDNEDDWEDVGSDEAGSDDEDLPQEYLYNDGIELHLPTGIKVGHRSLQRYYKQDLKPEVILTEGQGTLVAAETRSFLPAFDKKGVQTQQRVWQTERFDKKRLDKRSAKFVNNQPHYRDQLLQ | ||||||
Modified residue | 140 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Associates with nascent pre-60S particles that have not yet entered the translating pool, and is released from mature 60S subunits. Interacts with pre-60S factors ARX1 and RPL24. Interacts with JJJ1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P38344 | ARX1 Q03862 | 6 | EBI-21136, EBI-31385 | |
BINARY | P38344 | JJJ1 P53863 | 6 | EBI-21136, EBI-29183 | |
BINARY | P38344 | LSG1 P53145 | 6 | EBI-21136, EBI-23885 | |
BINARY | P38344 | NAP1 P25293 | 3 | EBI-21136, EBI-11850 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for zinc finger, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 7-31 | C2H2-type 1 | ||||
Sequence: YTCNSCVLTFDSSDEQRAHMKSDWH | ||||||
Region | 55-82 | Disordered | ||||
Sequence: SAAAASTSKSAEKEKPVTKKELKRREKQ | ||||||
Compositional bias | 66-82 | Basic and acidic residues | ||||
Sequence: EKEKPVTKKELKRREKQ | ||||||
Compositional bias | 102-118 | Polar residues | ||||
Sequence: NMQKSQEGNTPDLSKLS | ||||||
Region | 102-145 | Disordered | ||||
Sequence: NMQKSQEGNTPDLSKLSLQENEENKEKEEPKKEEPEQLTEEEMA | ||||||
Compositional bias | 119-145 | Basic and acidic residues | ||||
Sequence: LQENEENKEKEEPKKEEPEQLTEEEMA | ||||||
Zinc finger | 162-187 | C2H2-type 2 | ||||
Sequence: EQCLFCEHNKHFKDVEENLEHMFRTH | ||||||
Zinc finger | 215-239 | C2H2-type 3 | ||||
Sequence: NICIVCNYQGRTLTAVRQHMLAKRH | ||||||
Region | 268-293 | Disordered | ||||
Sequence: SNTTPDNEDDWEDVGSDEAGSDDEDL | ||||||
Compositional bias | 273-293 | Acidic residues | ||||
Sequence: DNEDDWEDVGSDEAGSDDEDL |
Sequence similarities
Belongs to the REI1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length393
- Mass (Da)45,862
- Last updated2011-07-27 v3
- ChecksumD01AA8FFDF4A0BAC
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 66-82 | Basic and acidic residues | ||||
Sequence: EKEKPVTKKELKRREKQ | ||||||
Compositional bias | 102-118 | Polar residues | ||||
Sequence: NMQKSQEGNTPDLSKLS | ||||||
Compositional bias | 119-145 | Basic and acidic residues | ||||
Sequence: LQENEENKEKEEPKKEEPEQLTEEEMA | ||||||
Sequence conflict | 152-153 | in Ref. 1; CAA49931 and 2; CAA85229 | ||||
Sequence: KL → NV | ||||||
Compositional bias | 273-293 | Acidic residues | ||||
Sequence: DNEDDWEDVGSDEAGSDDEDL |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X70529 EMBL· GenBank· DDBJ | CAA49931.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z36135 EMBL· GenBank· DDBJ | CAA85229.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07383.2 EMBL· GenBank· DDBJ | Genomic DNA |