P38238 · TRM7_YEAST
- ProteintRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- GeneTRM7
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids310 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs (PubMed:11927565, PubMed:22912484, PubMed:26310293, PubMed:31586407).
Requisite for faithful cytoplasmic translation (By similarity).
Requires TRM732 for methylation of the cytidine at position 32 of the anticodon loop of substrate tRNAs (PubMed:22912484).
Requires RTT10/TRM734 for methylation of the nucleotide at position 34 of the anticodon loop of substrate tRNAs (PubMed:22912484).
May modify position 32 in tRNA(Leu(UAA)), tRNA(Phe(GAA)), and tRNA(Trp(CCA)) and position 34 in tRNA(Leu(UAA)), tRNA(Phe(GAA)) and tRNA(Trp(CCA)) (PubMed:22912484).
Methylation of tRNA(Phe) at position 34 plays a role in the oxidative stress-response as it may promote translation of UUC over UUU, and UUC-containing genes are enriched for oxidative stress-responsive processes (PubMed:32053677).
Requisite for faithful cytoplasmic translation (By similarity).
Requires TRM732 for methylation of the cytidine at position 32 of the anticodon loop of substrate tRNAs (PubMed:22912484).
Requires RTT10/TRM734 for methylation of the nucleotide at position 34 of the anticodon loop of substrate tRNAs (PubMed:22912484).
May modify position 32 in tRNA(Leu(UAA)), tRNA(Phe(GAA)), and tRNA(Trp(CCA)) and position 34 in tRNA(Leu(UAA)), tRNA(Phe(GAA)) and tRNA(Trp(CCA)) (PubMed:22912484).
Methylation of tRNA(Phe) at position 34 plays a role in the oxidative stress-response as it may promote translation of UUC over UUU, and UUC-containing genes are enriched for oxidative stress-responsive processes (PubMed:32053677).
Miscellaneous
Present with 4110 molecules/cell in log phase SD medium.
Catalytic activity
- cytidine32/guanosine34 in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA + 2 S-adenosyl-L-homocysteine + 2 H+
RHEA-COMP:10246 CHEBI:74269 Position: 34CHEBI:82748 Position: 32+ 2 CHEBI:59789 = RHEA-COMP:10247 CHEBI:74445 Position: 34CHEBI:74495 Position: 32+ 2 CHEBI:57856 + 2 CHEBI:15378
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 25 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 55 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 56 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 57 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 83 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 99 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 124 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Active site | 164 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Molecular Function | S-adenosyl-L-methionine binding | |
Molecular Function | tRNA (cytidine(32)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (guanine(34)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (guanine) methyltransferase activity | |
Molecular Function | tRNA 2'-O-methyltransferase activity | |
Molecular Function | tRNA methyltransferase activity | |
Biological Process | cytoplasmic translation | |
Biological Process | tRNA methylation | |
Biological Process | tRNA nucleoside ribose methylation | |
Biological Process | wobble position ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP38238
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Leads to abnormal 2'-O-methylation and decreases charging of tRNA(Phe(GAA)) and tRNA(Leu(UAA)), resulting in translational defects and GCN2-dependent activation of the general amino acid control (GAAC) response (PubMed:22912484, PubMed:29596413).
Increases cellular ROS (reactive oxygen species) levels (PubMed:32053677).
Sensitive to oxidative stress induced by hydrogen peroxide, rotenone, and acetic acid (PubMed:32053677).
Increases RNA level of TRM7, TRM13 and TRM44 (PubMed:32053677).
Decreases cell population growth; the effect is suppressed by knockout of GCN2, GCN4 or MAF1 (PubMed:22912484, PubMed:25404562, PubMed:29596413, PubMed:32053677).
Increases cellular ROS (reactive oxygen species) levels (PubMed:32053677).
Sensitive to oxidative stress induced by hydrogen peroxide, rotenone, and acetic acid (PubMed:32053677).
Increases RNA level of TRM7, TRM13 and TRM44 (PubMed:32053677).
Decreases cell population growth; the effect is suppressed by knockout of GCN2, GCN4 or MAF1 (PubMed:22912484, PubMed:25404562, PubMed:29596413, PubMed:32053677).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 23 | Decreases cell population growth. | |||
Mutagenesis | 24 | Decreases cell population growth. | |||
Mutagenesis | 26 | Severely decreases methylation of guanosine in tRNA(Phe). Decreases cell population growth. | |||
Mutagenesis | 26 | Decreases methylation of cytidine in tRNA(Phe). | |||
Mutagenesis | 26 | Methylation of cytidine in tRNA(Phe) appears normal. | |||
Mutagenesis | 28 | Decreases cell population growth. | |||
Mutagenesis | 32 | Severely decreases methylation of guanosine in tRNA(Phe). Methylation of cytidine in tRNA(Phe) appears normal. | |||
Mutagenesis | 164 | Decreases cell population growth. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000155587 | 1-310 | tRNA (cytidine32/guanosine34-2'-O)-methyltransferase | ||
Proteomic databases
PTM databases
Interaction
Subunit
Heterodimer with TRM732; for 2'-O-methylation of cytidine 32 in substrate tRNAs (PubMed:22912484, PubMed:26310293).
Heterodimer with RTT10/TRM734; for 2'-O-methylation of position 34 in substrate tRNAs (PubMed:22912484, PubMed:26310293, PubMed:31586407).
Heterodimer with RTT10/TRM734; for 2'-O-methylation of position 34 in substrate tRNAs (PubMed:22912484, PubMed:26310293, PubMed:31586407).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 129-254 | Interacts with RTT10/TRM734 | |||
Region | 233-259 | Required for interaction with RTT10/TRM734 | |||
Region | 276-310 | Disordered | |||
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length310
- Mass (Da)34,701
- Last updated1994-10-01 v1
- Checksum3C7F2583F2012CCB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z35930 EMBL· GenBank· DDBJ | CAA85004.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07181.1 EMBL· GenBank· DDBJ | Genomic DNA |