P38238 · TRM7_YEAST

Function

function

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs (PubMed:11927565, PubMed:22912484, PubMed:26310293, PubMed:31586407).
Requisite for faithful cytoplasmic translation (By similarity).
Requires TRM732 for methylation of the cytidine at position 32 of the anticodon loop of substrate tRNAs (PubMed:22912484).
Requires RTT10/TRM734 for methylation of the nucleotide at position 34 of the anticodon loop of substrate tRNAs (PubMed:22912484).
May modify position 32 in tRNA(Leu(UAA)), tRNA(Phe(GAA)), and tRNA(Trp(CCA)) and position 34 in tRNA(Leu(UAA)), tRNA(Phe(GAA)) and tRNA(Trp(CCA)) (PubMed:22912484).
Methylation of tRNA(Phe) at position 34 plays a role in the oxidative stress-response as it may promote translation of UUC over UUU, and UUC-containing genes are enriched for oxidative stress-responsive processes (PubMed:32053677).

Miscellaneous

Present with 4110 molecules/cell in log phase SD medium.

Catalytic activity

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site25S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site55S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site56S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site57S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site83S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site99S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site124S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site164Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Molecular FunctionS-adenosyl-L-methionine binding
Molecular FunctiontRNA (cytidine(32)-2'-O)-methyltransferase activity
Molecular FunctiontRNA (guanine(34)-2'-O)-methyltransferase activity
Molecular FunctiontRNA (guanine) methyltransferase activity
Molecular FunctiontRNA 2'-O-methyltransferase activity
Molecular FunctiontRNA methyltransferase activity
Biological Processcytoplasmic translation
Biological ProcesstRNA methylation
Biological ProcesstRNA nucleoside ribose methylation
Biological Processwobble position ribose methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
  • EC number
  • Alternative names
    • 2'-O-ribose RNA methyltransferase TRM7
    • tRNA methylase 7

Gene names

    • Name
      TRM7
    • ORF names
      YBR0527
    • Ordered locus names
      YBR061C

Organism names

Accessions

  • Primary accession
    P38238
  • Secondary accessions
    • D6VQ61

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Leads to abnormal 2'-O-methylation and decreases charging of tRNA(Phe(GAA)) and tRNA(Leu(UAA)), resulting in translational defects and GCN2-dependent activation of the general amino acid control (GAAC) response (PubMed:22912484, PubMed:29596413).
Increases cellular ROS (reactive oxygen species) levels (PubMed:32053677).
Sensitive to oxidative stress induced by hydrogen peroxide, rotenone, and acetic acid (PubMed:32053677).
Increases RNA level of TRM7, TRM13 and TRM44 (PubMed:32053677).
Decreases cell population growth; the effect is suppressed by knockout of GCN2, GCN4 or MAF1 (PubMed:22912484, PubMed:25404562, PubMed:29596413, PubMed:32053677).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis23Decreases cell population growth.
Mutagenesis24Decreases cell population growth.
Mutagenesis26Severely decreases methylation of guanosine in tRNA(Phe). Decreases cell population growth.
Mutagenesis26Decreases methylation of cytidine in tRNA(Phe).
Mutagenesis26Methylation of cytidine in tRNA(Phe) appears normal.
Mutagenesis28Decreases cell population growth.
Mutagenesis32Severely decreases methylation of guanosine in tRNA(Phe). Methylation of cytidine in tRNA(Phe) appears normal.
Mutagenesis164Decreases cell population growth.

Variants

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The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001555871-310tRNA (cytidine32/guanosine34-2'-O)-methyltransferase

Proteomic databases

PTM databases

Interaction

Subunit

Heterodimer with TRM732; for 2'-O-methylation of cytidine 32 in substrate tRNAs (PubMed:22912484, PubMed:26310293).
Heterodimer with RTT10/TRM734; for 2'-O-methylation of position 34 in substrate tRNAs (PubMed:22912484, PubMed:26310293, PubMed:31586407).

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region129-254Interacts with RTT10/TRM734
Region233-259Required for interaction with RTT10/TRM734
Region276-310Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    310
  • Mass (Da)
    34,701
  • Last updated
    1994-10-01 v1
  • Checksum
    3C7F2583F2012CCB
MGKSSKDKRDLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQVLSRKLFDESPSSDKEDRKIVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNEKADFVCSDGAPDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLYSQLGYLFDKIVCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNKLCISDKLSHWNEEERNIAEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPPYKKALELKRSGKLTRSV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z35930
EMBL· GenBank· DDBJ
CAA85004.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006936
EMBL· GenBank· DDBJ
DAA07181.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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