P38205 · NCL1_YEAST

Function

function

Methylates cytosine to m5C at several positions in different tRNAs and pre-tRNAs containing intron. Able to modify tRNAs at all four positions (34, 40, 48 and 49) at which m5C has been found in tRNAs. May be involved in ribosome biogenesis as its disruption leads to increased sensitivity to the antibiotic paromomycin.

Miscellaneous

Present with 16100 molecules/cell in log phase SD medium.

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site173-179S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site202S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site229S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site257S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site310Nucleophile

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleolus
Cellular Componentnucleoplasm
Cellular Componentnucleus
Molecular FunctiontRNA (cytidine-5-)-methyltransferase activity
Molecular FunctiontRNA binding
Biological Processcellular response to hydrogen peroxide
Biological ProcesstRNA methylation
Biological ProcesstRNA modification
Biological ProcesstRNA wobble base cytosine methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multisite-specific tRNA:(cytosine-C(5))-methyltransferase
  • EC number
  • Alternative names
    • Multisite-specific tRNA:m5C-methyltransferase
    • tRNA (cytosine-5-)-methyltransferase NCL1
    • tRNA methyltransferase 4

Gene names

    • Name
      NCL1
    • Synonyms
      TRM4
    • ORF names
      YBL0437
    • Ordered locus names
      YBL024W

Organism names

Accessions

  • Primary accession
    P38205
  • Secondary accessions
    • D6VPX6

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002118201-684Multisite-specific tRNA:(cytosine-C5)-methyltransferase
Modified residue426Phosphothreonine
Modified residue431Phosphoserine
Modified residue667Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-24Disordered
Region650-684Disordered
Compositional bias652-667Basic and acidic residues
Compositional bias668-684Polar residues

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    684
  • Mass (Da)
    77,879
  • Last updated
    1994-10-01 v1
  • Checksum
    92FA9177CF4AB2CF
MARRKNFKKGNKKTFGARDDSRAQKNWSELVKENEKWEKYYKTLALFPEDQWEEFKKTCQAPLPLTFRITGSRKHAGEVLNLFKERHLPNLTNVEFEGEKIKAPVELPWYPDHLAWQLDVPKTVIRKNEQFAKTQRFLVVENAVGNISRQEAVSMIPPIVLEVKPHHTVLDMCAAPGSKTAQLIEALHKDTDEPSGFVVANDADARRSHMLVHQLKRLNSANLMVVNHDAQFFPRIRLHGNSNNKNDVLKFDRILCDVPCSGDGTMRKNVNVWKDWNTQAGLGLHAVQLNILNRGLHLLKNNGRLVYSTCSLNPIENEAVVAEALRKWGDKIRLVNCDDKLPGLIRSKGVSKWPVYDRNLTEKTKGDEGTLDSFFSPSEEEASKFNLQNCMRVYPHQQNTGGFFITVFEKVEDSTEAATEKLSSETPALESEGPQTKKIKVEEVQKKERLPRDANEEPFVFVDPQHEALKVCWDFYGIDNIFDRNTCLVRNATGEPTRVVYTVCPALKDVIQANDDRLKIIYSGVKLFVSQRSDIECSWRIQSESLPIMKHHMKSNRIVEANLEMLKHLLIESFPNFDDIRSKNIDNDFVEKMTKLSSGCAFIDVSRNDPAKENLFLPVWKGNKCINLMVCKEDTHELLYRIFGIDANAKATPSAEEKEKEKETTESPAETTTGTSTEAPSAAN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias652-667Basic and acidic residues
Compositional bias668-684Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X77291
EMBL· GenBank· DDBJ
CAA54502.1
EMBL· GenBank· DDBJ
Genomic DNA
Z35785
EMBL· GenBank· DDBJ
CAA84843.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006936
EMBL· GenBank· DDBJ
DAA07096.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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