P38205 · NCL1_YEAST
- ProteinMultisite-specific tRNA:(cytosine-C(5))-methyltransferase
- GeneNCL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids684 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methylates cytosine to m5C at several positions in different tRNAs and pre-tRNAs containing intron. Able to modify tRNAs at all four positions (34, 40, 48 and 49) at which m5C has been found in tRNAs. May be involved in ribosome biogenesis as its disruption leads to increased sensitivity to the antibiotic paromomycin.
Miscellaneous
Present with 16100 molecules/cell in log phase SD medium.
Catalytic activity
- cytidine34 in tRNA precursor + S-adenosyl-L-methionine = 5-methylcytidine34 in tRNA precursor + H+ + S-adenosyl-L-homocysteine
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 173-179 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: CAAPGSK | ||||||
Binding site | 202 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 229 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 257 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 310 | Nucleophile | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleolus | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Molecular Function | tRNA (cytidine-5-)-methyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | cellular response to hydrogen peroxide | |
Biological Process | tRNA methylation | |
Biological Process | tRNA modification | |
Biological Process | tRNA wobble base cytosine methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMultisite-specific tRNA:(cytosine-C(5))-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP38205
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000211820 | 1-684 | Multisite-specific tRNA:(cytosine-C5)-methyltransferase | |||
Sequence: MARRKNFKKGNKKTFGARDDSRAQKNWSELVKENEKWEKYYKTLALFPEDQWEEFKKTCQAPLPLTFRITGSRKHAGEVLNLFKERHLPNLTNVEFEGEKIKAPVELPWYPDHLAWQLDVPKTVIRKNEQFAKTQRFLVVENAVGNISRQEAVSMIPPIVLEVKPHHTVLDMCAAPGSKTAQLIEALHKDTDEPSGFVVANDADARRSHMLVHQLKRLNSANLMVVNHDAQFFPRIRLHGNSNNKNDVLKFDRILCDVPCSGDGTMRKNVNVWKDWNTQAGLGLHAVQLNILNRGLHLLKNNGRLVYSTCSLNPIENEAVVAEALRKWGDKIRLVNCDDKLPGLIRSKGVSKWPVYDRNLTEKTKGDEGTLDSFFSPSEEEASKFNLQNCMRVYPHQQNTGGFFITVFEKVEDSTEAATEKLSSETPALESEGPQTKKIKVEEVQKKERLPRDANEEPFVFVDPQHEALKVCWDFYGIDNIFDRNTCLVRNATGEPTRVVYTVCPALKDVIQANDDRLKIIYSGVKLFVSQRSDIECSWRIQSESLPIMKHHMKSNRIVEANLEMLKHLLIESFPNFDDIRSKNIDNDFVEKMTKLSSGCAFIDVSRNDPAKENLFLPVWKGNKCINLMVCKEDTHELLYRIFGIDANAKATPSAEEKEKEKETTESPAETTTGTSTEAPSAAN | ||||||
Modified residue | 426 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 431 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 667 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-24 | Disordered | ||||
Sequence: MARRKNFKKGNKKTFGARDDSRAQ | ||||||
Region | 650-684 | Disordered | ||||
Sequence: KATPSAEEKEKEKETTESPAETTTGTSTEAPSAAN | ||||||
Compositional bias | 652-667 | Basic and acidic residues | ||||
Sequence: TPSAEEKEKEKETTES | ||||||
Compositional bias | 668-684 | Polar residues | ||||
Sequence: PAETTTGTSTEAPSAAN |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length684
- Mass (Da)77,879
- Last updated1994-10-01 v1
- Checksum92FA9177CF4AB2CF
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 652-667 | Basic and acidic residues | ||||
Sequence: TPSAEEKEKEKETTES | ||||||
Compositional bias | 668-684 | Polar residues | ||||
Sequence: PAETTTGTSTEAPSAAN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X77291 EMBL· GenBank· DDBJ | CAA54502.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z35785 EMBL· GenBank· DDBJ | CAA84843.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07096.1 EMBL· GenBank· DDBJ | Genomic DNA |