P38137 · FAT2_YEAST

Function

function

Catalyzes the first step in a degradation pathway of oxalate to CO2 to protect the cell against the harmful effects of oxalate derived from endogenous processes or an environmental sources.

Miscellaneous

Present with 8770 molecules/cell in log phase SD medium.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
20 μMoxalate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
12 μmol/min/mg

pH Dependence

Optimum pH is 7.5.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site196-207ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentperoxisomal matrix
Cellular Componentperoxisomal membrane
Molecular FunctionATP binding
Molecular Functionmedium-chain fatty acid-CoA ligase activity
Molecular FunctionmRNA binding
Molecular Functionoxalate-CoA ligase activity
Biological Processfatty acid metabolic process
Biological Processoxalate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Oxalate--CoA ligase
  • EC number
  • Alternative names
    • Acyl-activating enzyme 3
    • Oxalyl-CoA synthetase
    • Peroxisomal-coenzyme A synthetase

Gene names

    • Name
      PCS60
    • Synonyms
      AAE3
      , FAT2
    • ORF names
      YBR1512
    • Ordered locus names
      YBR222C

Organism names

Accessions

  • Primary accession
    P38137
  • Secondary accessions
    • D6VQL9

Proteomes

Organism-specific databases

Subcellular Location

Peroxisome matrix
Peroxisome membrane
; Peripheral membrane protein
Note: Imported in peroxisome via recognition by the peroxisomal targeting signal receptor PEX5.

Keywords

Phenotypes & Variants

Disruption phenotype

Decreases recovery from exposure to oxalate, to oxalate-secreting microbes, and to oxidative stress.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001931821-543Oxalate--CoA ligase

Proteomic databases

PTM databases

Expression

Induction

By oleic acid.

Interaction

Subunit

Interacts with PEX5.

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif410-458FACS
Motif541-543C-terminal peroxisome targeting signal (PTS1)

Domain

The FACS motif is required for catalytic activity and substrate specificity.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    543
  • Mass (Da)
    60,489
  • Last updated
    1994-10-01 v1
  • Checksum
    2BA18B77D10D62F2
MTSAATVTASFNDTFSVSDNVAVIVPETDTQVTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAPLNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVEYDIYSPEDNYKRVIYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDGFHPKLFWDQFVKYNCNWFSCVPTISMIMLNMPKPNPFPHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLGYANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKISPIELDGIMLSHPKIDEAVAFGVPDDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHLASFKIPTKVYFVDKLPKTATGKIQRRVIAETFAKSSRNKSKL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z36091
EMBL· GenBank· DDBJ
CAA85185.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006936
EMBL· GenBank· DDBJ
DAA07339.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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