P38137 · FAT2_YEAST
- ProteinOxalate--CoA ligase
- GenePCS60
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids543 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the first step in a degradation pathway of oxalate to CO2 to protect the cell against the harmful effects of oxalate derived from endogenous processes or an environmental sources.
Miscellaneous
Present with 8770 molecules/cell in log phase SD medium.
Catalytic activity
- ATP + CoA + oxalate = AMP + diphosphate + oxalyl-CoA
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
20 μM | oxalate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
12 μmol/min/mg |
pH Dependence
Optimum pH is 7.5.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | peroxisomal matrix | |
Cellular Component | peroxisomal membrane | |
Molecular Function | ATP binding | |
Molecular Function | medium-chain fatty acid-CoA ligase activity | |
Molecular Function | mRNA binding | |
Molecular Function | oxalate-CoA ligase activity | |
Biological Process | fatty acid metabolic process | |
Biological Process | oxalate catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOxalate--CoA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP38137
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Peroxisome membrane ; Peripheral membrane protein
Note: Imported in peroxisome via recognition by the peroxisomal targeting signal receptor PEX5.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Decreases recovery from exposure to oxalate, to oxalate-secreting microbes, and to oxidative stress.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000193182 | 1-543 | Oxalate--CoA ligase | |||
Sequence: MTSAATVTASFNDTFSVSDNVAVIVPETDTQVTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAPLNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVEYDIYSPEDNYKRVIYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDGFHPKLFWDQFVKYNCNWFSCVPTISMIMLNMPKPNPFPHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLGYANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKISPIELDGIMLSHPKIDEAVAFGVPDDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHLASFKIPTKVYFVDKLPKTATGKIQRRVIAETFAKSSRNKSKL |
Proteomic databases
PTM databases
Expression
Induction
By oleic acid.
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 410-458 | FACS | ||||
Sequence: ENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKISPIELDGIMLSHPK | ||||||
Motif | 541-543 | C-terminal peroxisome targeting signal (PTS1) | ||||
Sequence: SKL |
Domain
The FACS motif is required for catalytic activity and substrate specificity.
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length543
- Mass (Da)60,489
- Last updated1994-10-01 v1
- Checksum2BA18B77D10D62F2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z36091 EMBL· GenBank· DDBJ | CAA85185.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07339.1 EMBL· GenBank· DDBJ | Genomic DNA |