P38086 · RDH54_YEAST

Function

function

Involved in the recombinational repair of double-strand breaks (DSB) in DNA during mitosis and meiosis. Has DNA dependent ATPase activity. Promotes D-loop (displacement loop) formation with RAD51 recombinase. Modifies the topology of double-stranded DNA during the D-loop reaction to facilitate the invasion of the homologous duplex molecule by the initiating single-stranded DNA substrate. Required for adaptation from G2/M checkpoint arrest induced by a double strand break, by participating in monitoring the extent of single-stranded DNA produced by resection of DNA ends. This role is distinct from its roles in recombination. Promotes colocalization of RAD51 and DMC1 during meiotic recombination. Involved in crossover interference.

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.

Caution

It is uncertain whether Met-1 or Met-35 is the initiator. In many S.cerevisiae strains, it is not possible to extend the sequence at the N-terminus beyond Met-35 because of a frameshift in the upstream sequence when compared to strain S288c. However, experimental evidence indicates that the longer protein is made in S288c.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site380-387ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionATP-dependent activity, acting on DNA
Molecular FunctionATP-dependent chromatin remodeler activity
Molecular FunctionDNA translocase activity
Molecular Functiondouble-stranded DNA binding
Molecular Functionhelicase activity
Biological ProcessDNA duplex unwinding
Biological ProcessDNA geometric change
Biological ProcessDNA recombination
Biological ProcessDNA repair
Biological Processdouble-strand break repair via homologous recombination
Biological Processheteroduplex formation
Biological Processmeiotic sister chromatid segregation
Biological Processreciprocal meiotic recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA repair and recombination protein RDH54
  • EC number
  • Alternative names
    • RAD homolog 54
    • Recombination factor TID1
    • Two hybrid interaction with DMC1 protein 1

Gene names

    • Name
      RDH54
    • Synonyms
      TID1
    • ORF names
      YBR0715
    • Ordered locus names
      YBR073W

Organism names

Accessions

  • Primary accession
    P38086
  • Secondary accessions
    • D6VQ73

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis3521%-2% of the ATPase activity.

Variants

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The viewer provides 16 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, cross-link.

TypeIDPosition(s)Description
ChainPRO_00000743461-958DNA repair and recombination protein RDH54
Cross-link649Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with RAD51 and DMC1.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, domain, motif.

TypeIDPosition(s)Description
Region189-217Disordered
Domain333-521Helicase ATP-binding
Motif506-509DEGH box
Domain665-824Helicase C-terminal

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    958
  • Mass (Da)
    107,946
  • Last updated
    2011-07-27 v4
  • Checksum
    B797375C08FA14FA
MAVISVKPRRREKILQEVKNSSVYQTVFDSGTTQMQIPKYENKPFKPPRRVGSNKYTQLKPTATAVTTAPISKAKVTVNLKRSISAGPTLNLAKKPNNLSSNENTRYFTIMYRKPTTKKHKTWSGDGYATLKASSDKLCFYNEAGKFLGSSMLPSDSDSLFETLFKAGSNEVQLDYELKENAEIRSAKEALSQNMGNPSPPTTSTTETVPSTKNDGGKYQMPLSQLFSLNTVKRFKSVTKQTNEHMTTVPKTSQNSKAKKYYPVFDVNKIDNPIVMNKNAAAEVDVIVDPLLGKFLRPHQREGVKFMYDCLMGLARPTIENPDIDCTTKSLVLENDSDISGCLLADDMGLGKTLMSITLIWTLIRQTPFASKVSCSQSGIPLTGLCKKILVVCPVTLIGNWKREFGKWLNLSRIGVLTLSSRNSPDMDKMAVRNFLKVQRTYQVLIIGYEKLLSVSEELEKNKHLIDMLVCDEGHRLKNGASKILNTLKSLDIRRKLLLTGTPIQNDLNEFFTIIDFINPGILGSFASFKRRFIIPITRARDTANRYNEELLEKGEERSKEMIEITKRFILRRTNAILEKYLPPKTDIILFCKPYSQQILAFKDILQGARLDFGQLTFSSSLGLITLLKKVCNSPGLVGSDPYYKSHIKDTQSQDSYSRSLNSGKLKVLMTLLEGIRKGTKEKVVVVSNYTQTLDIIENLMNMAGMSHCRLDGSIPAKQRDSIVTSFNRNPAIFGFLLSAKSGGVGLNLVGASRLILFDNDWNPSVDLQAMSRIHRDGQKKPCFIYRLVTTGCIDEKILQRQLMKNSLSQKFLGDSEMRNKESSNDDLFNKEDLKDLFSVHTDTKSNTHDLICSCDGLGEEIEYPETNQQQNTVELRKRSTTTWTSALDLQKKMNEAATNDDAKKSQYIRQCLVHYKHIDPARQDELFDEVITDSFTELKDSITFAFVKPGEICLREQ

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict752in Ref. 1; CAA53930 and 2; CAA85017

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X76294
EMBL· GenBank· DDBJ
CAA53930.1
EMBL· GenBank· DDBJ
Genomic DNA
Z35942
EMBL· GenBank· DDBJ
CAA85017.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006936
EMBL· GenBank· DDBJ
DAA07193.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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