P38051 · MENF_ECOLI
- ProteinIsochorismate synthase MenF
- GenemenF
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids431 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of chorismate to isochorismate. Can also catalyze the reverse reaction, but with a lower efficiency.
Catalytic activity
- chorismate = isochorismate
Cofactor
Note: Binds more tightly in the presence of chorismate.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
119 μM | isochorismate | 7.5 | 37 | |||
166.9 μM | chorismate | 7.5 | 37 | |||
192 μM | chorismate | |||||
195 μM | chorismate | |||||
770 μM | magnesium |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
91.7 pmol/sec/mg | 7.5 | 37 | with chorismate as substrate | ||
2.4 pmol/sec/mg | 7.5 | 37 | with isochorismate as substrate |
kcat is 213 min-1 for mutase activity with chorismate. kcat is 176 min-1 for mutase activity with chorismate in the presence of 30 mM of beta-mercaptoethanol (BME). kcat is 144.9 min-1 for mutase activity with chorismate (at pH 7.5 and 37 degrees Celsius). kcat is 80 min-1 for mutase activity with chorismate in the absence of 30 mM of beta-mercaptoethanol (BME). kcat is 3.8 min-1 for mutase activity with isochorismate (at pH 7.5 and 37 degrees Celsius).
pH Dependence
Optimum pH is between 7.5 and 8. The mutase activity decreases sharply at pH 6.5 and 9 to about 20% of that observed at the optimum pH.
Temperature Dependence
Optimum temperature is 37 degrees Celsius. At 60 degrees Celsius the mutase activity is completely lost.
Pathway
Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 1/7.
Quinol/quinone metabolism; menaquinone biosynthesis.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | isochorismate synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | menaquinone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameIsochorismate synthase MenF
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP38051
- Secondary accessions
Proteomes
Phenotypes & Variants
Disruption phenotype
Mutant produces only a trace of menaquinone.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 190 | Lack of activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 240 | Lack of activity. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 255 | Decrease in activity. | ||||
Sequence: L → A | ||||||
Mutagenesis | 344 | Lack of activity. | ||||
Sequence: A → T | ||||||
Mutagenesis | 387 | Lack of activity. | ||||
Sequence: R → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000154147 | 1-431 | Isochorismate synthase MenF | |||
Sequence: MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length431
- Mass (Da)48,765
- Last updated1997-11-01 v4
- ChecksumD081724F3329FE47
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 310-312 | in Ref. 3; AAC44303 | ||||
Sequence: VLR → YC |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z50849 EMBL· GenBank· DDBJ | CAA90702.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U54790 EMBL· GenBank· DDBJ | AAC44303.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75325.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16092.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U58768 EMBL· GenBank· DDBJ | AAB02729.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M21787 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |