P37188 · PTKB_ECOLI
- ProteinPTS system galactitol-specific EIIB component
- GenegatB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. The enzyme II complex composed of GatA, GatB and GatC is involved in galactitol transport. It can also use D-glucitol.
Catalytic activity
- galactitol(out) + N(pros)-phospho-L-histidyl-[protein] = galactitol 1-phosphate(in) + L-histidyl-[protein]
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
4.4 μM | D-galactitol | |||||
800 μM | D-glucitol |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
3.2 nmol/min/mg | with D-galactitol as substrate | ||||
3.2 nmol/min/mg | with D-glucitol as substrate |
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 9 | Phosphocysteine intermediate; for EIIB activity | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | transmembrane transporter complex | |
Molecular Function | protein-N(PI)-phosphohistidine-sugar phosphotransferase activity | |
Molecular Function | protein-phosphocysteine-galactitol-phosphotransferase system transporter activity | |
Biological Process | galactitol metabolic process | |
Biological Process | galactitol transmembrane transport | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePTS system galactitol-specific EIIB component
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP37188
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000186572 | 1-94 | PTS system galactitol-specific EIIB component | |||
Sequence: MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEALQNKILTILQG | ||||||
Modified residue | 9 | Phosphocysteine; by EIIA | ||||
Sequence: C |
Keywords
- PTM
Proteomic databases
Expression
Induction
Constitutively expressed.
Interaction
Subunit
Forms a complex with one each of subunit of GatA, GatB and 2 subunits of GatC.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-94 | PTS EIIB type-2 | ||||
Sequence: MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEIETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEALQNKILTILQG |
Domain
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length94
- Mass (Da)10,222
- Last updated1997-11-01 v2
- ChecksumA8156F04894F0CD7
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 9 | in Ref. 5; AA sequence | ||||
Sequence: C → E | ||||||
Sequence conflict | 29 | in Ref. 1; CAA56229 | ||||
Sequence: N → S | ||||||
Sequence conflict | 59 | in Ref. 1; CAA56229 | ||||
Sequence: K → R | ||||||
Sequence conflict | 76 | in Ref. 1; CAA56229 | ||||
Sequence: I → V | ||||||
Sequence conflict | 79 | in Ref. 1; CAA56229 | ||||
Sequence: I → V |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X79837 EMBL· GenBank· DDBJ | CAA56229.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75154.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA15956.1 EMBL· GenBank· DDBJ | Genomic DNA |