P37106 · SR541_ARATH

Function

function

Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). As part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane. Binds to the signal sequence of presecretory proteins when they emerge from the ribosomes. Displays basal GTPase activity, and stimulates reciprocal GTPase activation of the SR subunit SRPRA. Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SR subunit SRPRA. SR compaction and GTPase mediated rearrangement of SR drive SRP-mediated cotranslational protein translocation into the ER (By similarity).
Requires the presence of SRP9/SRP14 and/or SRP19 to stably interact with RNA (By similarity).

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site108-115GTP (UniProtKB | ChEBI)
Binding site190-194GTP (UniProtKB | ChEBI)
Binding site248-251GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentendoplasmic reticulum
Cellular Componentsignal recognition particle, endoplasmic reticulum targeting
Molecular Function7S RNA binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Biological ProcessSRP-dependent cotranslational protein targeting to membrane

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Signal recognition particle subunit SRP54 1
  • EC number
  • Alternative names
    • Signal recognition particle 54 kDa protein 1 (SRP54)

Gene names

    • Name
      SRP-54A
    • Synonyms
      SRP-54
    • ORF names
      F9L1.25
    • Ordered locus names
      At1g15310

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P37106

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001012041-479Signal recognition particle subunit SRP54 1

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Component of a signal recognition particle (SRP) complex that consists of a 7SL RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-295G-domain
Region296-479M-domain

Domain

The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit SRPRA. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.
The M domain binds the 7SL RNA in presence of SRP19 and binds the signal sequence of presecretory proteins.

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    479
  • Mass (Da)
    53,007
  • Last updated
    1994-10-01 v1
  • Checksum
    DA4C0634420F486E
MVLAELGGRITRAIQQMNNVTIIDEKVLNDFLNEITRALLQSDVSFGLVEKMQTNIKKIVNLDDLAAGHNKRLIIEQAIFKELCRMLDPGKPAFAPKKAKPSVVMFVGLQGAGKTTTCTKYAYYHQKKGYKAALVCADTFRAGAFDQLKQNATKAKIPFYGSYTESDPVKIAVEGVDRFKKEKCDLIIVDTSGRHKQAASLFEEMRQVAEATEPDLVIFVMDSSIGQAAFEQAEAFKETVSVGAVIITKMDGHAKGGGALSAVAATKSPVIFIGTGEHMDEFEVFDVKPFVSRLLGKGDWSGLVDKLQEVVPKDLQNELVENLSQGNFTLRSMYDQFQCSLRIPLNQLFSMLPGISAEMMPKGHGEESRVKMKRYMTMMDSMTNKELDSPNPKIFNESRIMRIARGSGRLVREVMEMLEEYKRIAKTMKGIKIPKNGDMSKVIPPQMLKQMGGMSGLQSLMKQMGSAKDMMGMFGGGGK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L19997
EMBL· GenBank· DDBJ
AAA19728.1
EMBL· GenBank· DDBJ
Unassigned DNA
AC007591
EMBL· GenBank· DDBJ
AAD39659.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE29301.1
EMBL· GenBank· DDBJ
Genomic DNA
AF344329
EMBL· GenBank· DDBJ
AAK06880.1
EMBL· GenBank· DDBJ
mRNA
AY086187
EMBL· GenBank· DDBJ
AAM64266.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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