P36568 · BIOA_SERMA
- ProteinAdenosylmethionine-8-amino-7-oxononanoate aminotransferase
- GenebioA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids425 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.
Catalytic activity
- (8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 19 | Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM | ||||
Sequence: Y | ||||||
Binding site | 54 | substrate | ||||
Sequence: W | ||||||
Binding site | 114-115 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: GS | ||||||
Binding site | 146 | substrate | ||||
Sequence: Y | ||||||
Binding site | 248 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 277 | substrate | ||||
Sequence: K | ||||||
Binding site | 310 | substrate | ||||
Sequence: G | ||||||
Binding site | 311-312 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: PT | ||||||
Binding site | 394 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenosylmethionine-8-amino-7-oxononanoate transaminase activity | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenosylmethionine-8-amino-7-oxononanoate aminotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Serratia
Accessions
- Primary accessionP36568
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000120376 | 1-425 | Adenosylmethionine-8-amino-7-oxononanoate aminotransferase | |||
Sequence: MSVTASDLAFDQRHIWHPYTSMSRPLPCYPIESASGVELQLADGRSLVDGMSSWWAAIHGYNHPRLNQAASQQLEKMSHVMFGGITHPAAISLCRRLVEMTPEALQCVFLADSGSVAVEVSLKMALQYWQARGERRQRILTLRHGYHGDTFGAMSVCDPDNSMHSLYQGYLAPHLFATAPQCRFDEEWREEDIAPFAALLEQHAGEVAAVILEPVVQGAGGMRIYHPTYLKRVRDVVRVAIKLLLIADEIATGFGRTGKLFACEHAQVVPDILCLGKALTGGYMTLSATLTTRHVAETISNGAAGCFMHGPTFMGNPLACAVADASLALLAENRWQAQVSAIETQLKRELLPLGRCRRWPHVRVLGAIGVVEMREPVDVAELQRGFVERGVWIRPFGKLIYLMPPYIIEAEQLSRLTAAVADAAR | ||||||
Modified residue | 277 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length425
- Mass (Da)46,867
- Last updated1994-06-01 v1
- Checksum8BA657E59BB94AF6