P36304 · POLN_KYMVJ
- ProteinNon-structural replication polyprotein
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1874 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Methyltransferase/Protease
Acts as a cysteine protease, methyltransferase and deubiquitinase (By similarity).
The cysteine protease activity cleaves the polyprotein giving rise to mature proteins (By similarity).
The methyltransferase domain is probably involved in viral RNA capping (By similarity).
The cysteine protease activity cleaves the polyprotein giving rise to mature proteins (By similarity).
The methyltransferase domain is probably involved in viral RNA capping (By similarity).
RNA-directed RNA polymerase
RNA-directed RNA polymerase is responsible for the replication and transcription of the genome.
Catalytic activity
RNA-directed RNA polymerase
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Features
Showing features for active site, site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | cysteine-type endopeptidase activity | |
Molecular Function | mRNA methyltransferase activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | DNA-templated transcription | |
Biological Process | methylation | |
Biological Process | mRNA modification | |
Biological Process | proteolysis | |
Biological Process | RNA processing | |
Biological Process | symbiont-mediated perturbation of host ubiquitin-like protein modification | |
Biological Process | viral RNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameNon-structural replication polyprotein
- Cleaved into 3 chains
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Tymovirales > Tymoviridae > Tymovirus > Tymovirus kennedyae
- Virus hosts
Accessions
- Primary accessionP36304
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000460988 | 1-?905 | Methyltransferase/Protease | |||
Sequence: MAFQLALDALASTSHKDPSLHPVLESVHDSLTDSLQTYPWMVPQDLQPFLLKSGIPINSFGSSPHPHPAHKTLETHLLFTHWMHLCTQPSSVLFMKPQKFMKLQRKNKFFQHLHNYRLTPTDSVRFPSTSPHLPNTPFVFMHDALMYYQPEQILHLFHQVPQLTNLFCSLVTPPESHFTHLSLMPDLYTFTLKGQTLHYTPEGHSAGSYNQPITALSWLKINSILSPNLNLSITILESWGPLHSILIQRGLPLPDPKLLVRSLPPFSRSPDPETDLVSFQVPKSVELPQATFLSQPLRHRLVPESVYNALFTYTRAVRTLRVSDPAGFVRTQSNKPEHKWVTPSAWDNLQTFALLNCPLRPNVVYHVLLNPLQKMKLYFSQHWRRLGVIAAPGLFCLSLLLRSQKWSLPLPKAKSISVFRRNLLLPPKPHRPPLLPHPEQMLQEFKLPWHRPPPKGKRNPFLTLLINLLHIPREICAGIRRYPSYYQSIQPKPLNPIQQFRNQLLAQLHTLPLPKKFLSILPRARKDIPLLPRMSFTTVNLPLQPPMWLAIGASLVPELAFLLSWLSGDVDLQTQHDIYHHHLHPENFTLSWTRTPYLALAPSPFLPYAHSPLPPLPVNSSPLFPPPPPLPPSQPPLSQGPATQAPSAQPTPGEPLLAPPTTELKPESSNPNNPNPSSSAGSNPPPKSSSSDNPPAPNKPTPTSSSTTPPSPNLPLQFGSIHSPFLSDGQLNYSALPPPQDPTNTTLSLLPEPKPPTEVQSPLMADPTCVGPAVSFSSLYPRDFFPNTASFLTRLRLSPPTPLPMPKNNCLLTAVAPSLHINPHRLWTSLQEVLPDSLLSNSEIDSVGMSTDLLTALSHLFNFQAVVHSERGDILFGLQSAKTVIHIYHTNGPPAHYSPPPKIIG | ||||||
Chain | PRO_0000222935 | 1-1874 | Non-structural replication polyprotein | |||
Sequence: MAFQLALDALASTSHKDPSLHPVLESVHDSLTDSLQTYPWMVPQDLQPFLLKSGIPINSFGSSPHPHPAHKTLETHLLFTHWMHLCTQPSSVLFMKPQKFMKLQRKNKFFQHLHNYRLTPTDSVRFPSTSPHLPNTPFVFMHDALMYYQPEQILHLFHQVPQLTNLFCSLVTPPESHFTHLSLMPDLYTFTLKGQTLHYTPEGHSAGSYNQPITALSWLKINSILSPNLNLSITILESWGPLHSILIQRGLPLPDPKLLVRSLPPFSRSPDPETDLVSFQVPKSVELPQATFLSQPLRHRLVPESVYNALFTYTRAVRTLRVSDPAGFVRTQSNKPEHKWVTPSAWDNLQTFALLNCPLRPNVVYHVLLNPLQKMKLYFSQHWRRLGVIAAPGLFCLSLLLRSQKWSLPLPKAKSISVFRRNLLLPPKPHRPPLLPHPEQMLQEFKLPWHRPPPKGKRNPFLTLLINLLHIPREICAGIRRYPSYYQSIQPKPLNPIQQFRNQLLAQLHTLPLPKKFLSILPRARKDIPLLPRMSFTTVNLPLQPPMWLAIGASLVPELAFLLSWLSGDVDLQTQHDIYHHHLHPENFTLSWTRTPYLALAPSPFLPYAHSPLPPLPVNSSPLFPPPPPLPPSQPPLSQGPATQAPSAQPTPGEPLLAPPTTELKPESSNPNNPNPSSSAGSNPPPKSSSSDNPPAPNKPTPTSSSTTPPSPNLPLQFGSIHSPFLSDGQLNYSALPPPQDPTNTTLSLLPEPKPPTEVQSPLMADPTCVGPAVSFSSLYPRDFFPNTASFLTRLRLSPPTPLPMPKNNCLLTAVAPSLHINPHRLWTSLQEVLPDSLLSNSEIDSVGMSTDLLTALSHLFNFQAVVHSERGDILFGLQSAKTVIHIYHTNGPPAHYSPPPKIIGSNSPPSSQQHPLEQAALRFKYQGSHLPFSSFHSFTTSVQHAKNLISNMKNGFDGVMSTIEPSIRHQPGHSPREKFIALDAMIDLARPKTVSMFHLAGFAGCGKTKPLQSLLSTRPFHSFRVSTPTTELRNEWKKDMNLPASQAFRFCTWESSLLKQTKILVIDEIYKLPRGYLDLCILADPCLELVIILGDPLQGEYHSTSPHSSNHQLQSETTRLLPFIDHYCWWTYRVPSHIADLFSVPSFNRSEGHYQMAVRTADSYTPGHFNLVNSVATANAVIQLGFPATTISASQGVTHHNRVTILLDKHSRLLSPSNTLVALTRSTVGVEFLGDIGSLSGTNNSSDMFSRAIYRQPINLSSSFPRIFHLLPLLNKPISRRSTRLIGSHSPIFHNPRLTNIHLPPHIPTSYSQDFVVSNPIFQGQADPRLDTHFLPPTRLPLQSELLPAQLSQTTKPTDSFTNNTPFTPVYPGENFENLAAFFLPAHDPELKEVTRRDQTSAQFPWFDRPFSLSCQPSSLIAAKHSPSQDPTLLPFSIPKRLRFRKSDNPHVLSAIDVLLGNQLFFNLCKAYRRNPTHVGPFNPALFAECIALNDYAQLSSKTQATLVANHSRSDPDWRHTAVKIFAKSQHKVNDASIFGNWKACQTLALMHDFVILSLGPVKKYQRIFDALDRPPHLYTHCGKSPADLSAWCQTHLTGQIKLTNDYTAFDQSQHGESVILEALKMKRLSIPSHLIQLHVHLKTNVATQFGPLTCMRLTGEPGTYDDNSDYNLAVIHSQFDMKDIPVMVSGDDSLIDRQPPLAQSWEATKRLLHLRFKTEKTTHPLFCGYYTGSAGAIRNPLALFSKLMIAIDDEAIHDRRLSYLTEFSTGHQLGDALWTLLPESTQIYQSACFDYFCRHSPPHEKALLSSFELPDSVISKISSSTKWLSKNAFYALPSKIRKAVIASRHSSSFPENPDVSQLEFELLQSFQF | ||||||
Chain | PRO_0000460989 | ?906-?1288 | Putative helicase | |||
Sequence: SNSPPSSQQHPLEQAALRFKYQGSHLPFSSFHSFTTSVQHAKNLISNMKNGFDGVMSTIEPSIRHQPGHSPREKFIALDAMIDLARPKTVSMFHLAGFAGCGKTKPLQSLLSTRPFHSFRVSTPTTELRNEWKKDMNLPASQAFRFCTWESSLLKQTKILVIDEIYKLPRGYLDLCILADPCLELVIILGDPLQGEYHSTSPHSSNHQLQSETTRLLPFIDHYCWWTYRVPSHIADLFSVPSFNRSEGHYQMAVRTADSYTPGHFNLVNSVATANAVIQLGFPATTISASQGVTHHNRVTILLDKHSRLLSPSNTLVALTRSTVGVEFLGDIGSLSGTNNSSDMFSRAIYRQPINLSSSFPRIFHLLPLLNKPISRRSTRLIG | ||||||
Chain | PRO_0000460990 | ?1289-1874 | RNA-directed RNA polymerase | |||
Sequence: SHSPIFHNPRLTNIHLPPHIPTSYSQDFVVSNPIFQGQADPRLDTHFLPPTRLPLQSELLPAQLSQTTKPTDSFTNNTPFTPVYPGENFENLAAFFLPAHDPELKEVTRRDQTSAQFPWFDRPFSLSCQPSSLIAAKHSPSQDPTLLPFSIPKRLRFRKSDNPHVLSAIDVLLGNQLFFNLCKAYRRNPTHVGPFNPALFAECIALNDYAQLSSKTQATLVANHSRSDPDWRHTAVKIFAKSQHKVNDASIFGNWKACQTLALMHDFVILSLGPVKKYQRIFDALDRPPHLYTHCGKSPADLSAWCQTHLTGQIKLTNDYTAFDQSQHGESVILEALKMKRLSIPSHLIQLHVHLKTNVATQFGPLTCMRLTGEPGTYDDNSDYNLAVIHSQFDMKDIPVMVSGDDSLIDRQPPLAQSWEATKRLLHLRFKTEKTTHPLFCGYYTGSAGAIRNPLALFSKLMIAIDDEAIHDRRLSYLTEFSTGHQLGDALWTLLPESTQIYQSACFDYFCRHSPPHEKALLSSFELPDSVISKISSSTKWLSKNAFYALPSKIRKAVIASRHSSSFPENPDVSQLEFELLQSFQF |
Post-translational modification
Specific enzymatic cleavages by the host yield mature proteins.
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 58-219 | Alphavirus-like MT | ||||
Sequence: NSFGSSPHPHPAHKTLETHLLFTHWMHLCTQPSSVLFMKPQKFMKLQRKNKFFQHLHNYRLTPTDSVRFPSTSPHLPNTPFVFMHDALMYYQPEQILHLFHQVPQLTNLFCSLVTPPESHFTHLSLMPDLYTFTLKGQTLHYTPEGHSAGSYNQPITALSWL | ||||||
Compositional bias | 619-639 | Pro residues | ||||
Sequence: NSSPLFPPPPPLPPSQPPLSQ | ||||||
Region | 619-760 | Disordered | ||||
Sequence: NSSPLFPPPPPLPPSQPPLSQGPATQAPSAQPTPGEPLLAPPTTELKPESSNPNNPNPSSSAGSNPPPKSSSSDNPPAPNKPTPTSSSTTPPSPNLPLQFGSIHSPFLSDGQLNYSALPPPQDPTNTTLSLLPEPKPPTEVQ | ||||||
Compositional bias | 663-687 | Polar residues | ||||
Sequence: ELKPESSNPNNPNPSSSAGSNPPPK | ||||||
Compositional bias | 688-709 | Pro residues | ||||
Sequence: SSSSDNPPAPNKPTPTSSSTTP | ||||||
Compositional bias | 710-751 | Polar residues | ||||
Sequence: PSPNLPLQFGSIHSPFLSDGQLNYSALPPPQDPTNTTLSLLP | ||||||
Domain | 757-912 | Peptidase C21 | ||||
Sequence: TEVQSPLMADPTCVGPAVSFSSLYPRDFFPNTASFLTRLRLSPPTPLPMPKNNCLLTAVAPSLHINPHRLWTSLQEVLPDSLLSNSEIDSVGMSTDLLTALSHLFNFQAVVHSERGDILFGLQSAKTVIHIYHTNGPPAHYSPPPKIIGSNSPPSS | ||||||
Domain | 972-1129 | +RNA virus helicase ATP-binding | ||||
Sequence: PGHSPREKFIALDAMIDLARPKTVSMFHLAGFAGCGKTKPLQSLLSTRPFHSFRVSTPTTELRNEWKKDMNLPASQAFRFCTWESSLLKQTKILVIDEIYKLPRGYLDLCILADPCLELVIILGDPLQGEYHSTSPHSSNHQLQSETTRLLPFIDHYC | ||||||
Domain | 1130-1266 | +RNA virus helicase C-terminal | ||||
Sequence: WWTYRVPSHIADLFSVPSFNRSEGHYQMAVRTADSYTPGHFNLVNSVATANAVIQLGFPATTISASQGVTHHNRVTILLDKHSRLLSPSNTLVALTRSTVGVEFLGDIGSLSGTNNSSDMFSRAIYRQPINLSSSFP | ||||||
Domain | 1601-1707 | RdRp catalytic | ||||
Sequence: QIKLTNDYTAFDQSQHGESVILEALKMKRLSIPSHLIQLHVHLKTNVATQFGPLTCMRLTGEPGTYDDNSDYNLAVIHSQFDMKDIPVMVSGDDSLIDRQPPLAQSW |
Sequence similarities
Belongs to the Tymoviridae non-structural replication polyprotein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,874
- Mass (Da)210,030
- Last updated1994-06-01 v1
- Checksum72B6DDE57732A2AD
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 619-639 | Pro residues | ||||
Sequence: NSSPLFPPPPPLPPSQPPLSQ | ||||||
Compositional bias | 663-687 | Polar residues | ||||
Sequence: ELKPESSNPNNPNPSSSAGSNPPPK | ||||||
Compositional bias | 688-709 | Pro residues | ||||
Sequence: SSSSDNPPAPNKPTPTSSSTTP | ||||||
Compositional bias | 710-751 | Polar residues | ||||
Sequence: PSPNLPLQFGSIHSPFLSDGQLNYSALPPPQDPTNTTLSLLP |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D00637 EMBL· GenBank· DDBJ | BAA00532.1 EMBL· GenBank· DDBJ | Genomic RNA |