P36096 · TUL1_YEAST
- ProteinTransmembrane E3 ubiquitin-protein ligase 1
- GeneTUL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids758 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalytic component of the DSC E3 ubiquitin ligase complexes that tag proteins present in Golgi, endosome and vacuole membranes and function in protein homeostasis under non-stress conditions and support a role in protein quality control (PubMed:25078903, PubMed:29355480).
Mediates ubiquitination of vacuolar proteins such as CPS1, PPN1, PEP12 and other proteins containing exposed hydrophilic residues within their transmembrane domains, leading to their sorting into internal vesicles in late endosomes (PubMed:11788821).
Targets also the unpalmitoylated endosomal SNARE TLG1 to the MVB pathway (PubMed:15973437).
Mediates ubiquitination of vacuolar proteins such as CPS1, PPN1, PEP12 and other proteins containing exposed hydrophilic residues within their transmembrane domains, leading to their sorting into internal vesicles in late endosomes (PubMed:11788821).
Targets also the unpalmitoylated endosomal SNARE TLG1 to the MVB pathway (PubMed:15973437).
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Dsc E3 ubiquitin ligase complex | |
Cellular Component | endomembrane system | |
Cellular Component | fungal-type vacuole | |
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | ubiquitin-protein transferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | proteasome-mediated ubiquitin-dependent protein catabolic process | |
Biological Process | protein ubiquitination | |
Biological Process | ubiquitin-dependent protein catabolic process | |
Biological Process | ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTransmembrane E3 ubiquitin-protein ligase 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP36096
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 27-398 | Lumenal | ||||
Sequence: VSSQYEFNQLQRLKQQFRTEHNTFVNMTYTDSFRNITGLKLSYQDMLNNPLQNATYPLPGKDYDRWFPNQNYMVLPNDVIEAINTEVWNTSNDDASNLFPPNITSTLLGKIDLVSNNKYEKIRMPVPRFYEPATDFSEDIPPEGETYWSEWPSYGELHNVSFQHGEIAIQISHMSNLQDNNNYLRRNFINKKNDRWKLLNLQIDFSDKAEKEKHSIYSKAVYDIQRGRILSISQSSKFHSLFALPHYMSFQNDYNEKIFNDVKELVDEFWNFTDYTDVMTMKDVQDAYNNANFKCEYLIFLQLEPWNQYTRDQIKLIDDELNWPLGRPANLSSLPPINVVSGLLYSPDCGVRLGLHNVKGTRYELKIMSIRK | ||||||
Transmembrane | 399-419 | Helical | ||||
Sequence: HLLFGIALFAAQIYLLLTQMH | ||||||
Topological domain | 420-431 | Cytoplasmic | ||||
Sequence: HTNTPSMVNKIS | ||||||
Transmembrane | 432-452 | Helical | ||||
Sequence: FYCFSMINLVDGSLATLYFVA | ||||||
Topological domain | 453-458 | Lumenal | ||||
Sequence: ASVVPE | ||||||
Transmembrane | 459-479 | Helical | ||||
Sequence: LYLPLVISAFSCFILASIFEI | ||||||
Topological domain | 480-523 | Cytoplasmic | ||||
Sequence: RYLISIYASQVNEQNVGIINLLRGNTGTYDENRPRPAFIPDEGS | ||||||
Transmembrane | 524-544 | Helical | ||||
Sequence: IGGSLYGRFFFMLIIFTFLIL | ||||||
Topological domain | 545-553 | Lumenal | ||||
Sequence: SSTSWPRQL | ||||||
Transmembrane | 554-574 | Helical | ||||
Sequence: RMVFEYILIFILNSYWIPQIF | ||||||
Topological domain | 575-602 | Cytoplasmic | ||||
Sequence: RNAVKGIPSRRERARSSIGGNRSQNKMP | ||||||
Transmembrane | 603-623 | Helical | ||||
Sequence: LLWSFVIGTTIIRSLPVVYVF | ||||||
Topological domain | 624-635 | Lumenal | ||||
Sequence: TYSSNVFRHHKD | ||||||
Transmembrane | 636-656 | Helical | ||||
Sequence: VHFVVFLSLWLLFQISILYSQ | ||||||
Topological domain | 657-758 | Cytoplasmic | ||||
Sequence: DVLGSRWFLPKHTIPDGYSYFKPLSNEYISEHGGGTAEHTVDCAICMSDVPIYIEEIPETHKVDQHSYMVTPCNHVFHTSCLENWMNYKLQCPVCRSPLPPL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-26 | |||||
Sequence: MEIDGNTLVFIIVILFLFFSSPGGDG | ||||||
Chain | PRO_0000056409 | 27-758 | Transmembrane E3 ubiquitin-protein ligase 1 | |||
Sequence: VSSQYEFNQLQRLKQQFRTEHNTFVNMTYTDSFRNITGLKLSYQDMLNNPLQNATYPLPGKDYDRWFPNQNYMVLPNDVIEAINTEVWNTSNDDASNLFPPNITSTLLGKIDLVSNNKYEKIRMPVPRFYEPATDFSEDIPPEGETYWSEWPSYGELHNVSFQHGEIAIQISHMSNLQDNNNYLRRNFINKKNDRWKLLNLQIDFSDKAEKEKHSIYSKAVYDIQRGRILSISQSSKFHSLFALPHYMSFQNDYNEKIFNDVKELVDEFWNFTDYTDVMTMKDVQDAYNNANFKCEYLIFLQLEPWNQYTRDQIKLIDDELNWPLGRPANLSSLPPINVVSGLLYSPDCGVRLGLHNVKGTRYELKIMSIRKHLLFGIALFAAQIYLLLTQMHHTNTPSMVNKISFYCFSMINLVDGSLATLYFVAASVVPELYLPLVISAFSCFILASIFEIRYLISIYASQVNEQNVGIINLLRGNTGTYDENRPRPAFIPDEGSIGGSLYGRFFFMLIIFTFLILSSTSWPRQLRMVFEYILIFILNSYWIPQIFRNAVKGIPSRRERARSSIGGNRSQNKMPLLWSFVIGTTIIRSLPVVYVFTYSSNVFRHHKDVHFVVFLSLWLLFQISILYSQDVLGSRWFLPKHTIPDGYSYFKPLSNEYISEHGGGTAEHTVDCAICMSDVPIYIEEIPETHKVDQHSYMVTPCNHVFHTSCLENWMNYKLQCPVCRSPLPPL |
Proteomic databases
Interaction
Subunit
Component of the DSC E3 ligase complexes composed of at least TUL1, DSC2, DSC3, UBX3, CDC48 as well as VLD1 for the vacuole-localized complex or GLD1 for the Golgi/endosome-localized complex (PubMed:25078903, PubMed:29355480).
Interacts with UBC4 (PubMed:11788821).
Interacts with UBC4 (PubMed:11788821).
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 699-752 | RING-type; atypical | ||||
Sequence: CAICMSDVPIYIEEIPETHKVDQHSYMVTPCNHVFHTSCLENWMNYKLQCPVCR |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length758
- Mass (Da)87,880
- Last updated1994-06-01 v1
- ChecksumF29A61E994F96628
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X71622 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
X69584 EMBL· GenBank· DDBJ | CAA49298.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z28034 EMBL· GenBank· DDBJ | CAA81869.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006944 EMBL· GenBank· DDBJ | DAA09121.1 EMBL· GenBank· DDBJ | Genomic DNA |