P36096 · TUL1_YEAST

Function

function

Catalytic component of the DSC E3 ubiquitin ligase complexes that tag proteins present in Golgi, endosome and vacuole membranes and function in protein homeostasis under non-stress conditions and support a role in protein quality control (PubMed:25078903, PubMed:29355480).
Mediates ubiquitination of vacuolar proteins such as CPS1, PPN1, PEP12 and other proteins containing exposed hydrophilic residues within their transmembrane domains, leading to their sorting into internal vesicles in late endosomes (PubMed:11788821).
Targets also the unpalmitoylated endosomal SNARE TLG1 to the MVB pathway (PubMed:15973437).

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

GO annotations

AspectTerm
Cellular ComponentDsc E3 ubiquitin ligase complex
Cellular Componentendomembrane system
Cellular Componentfungal-type vacuole
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Molecular Functionubiquitin protein ligase activity
Molecular Functionubiquitin-protein transferase activity
Molecular Functionzinc ion binding
Biological Processproteasome-mediated ubiquitin-dependent protein catabolic process
Biological Processprotein ubiquitination
Biological Processubiquitin-dependent protein catabolic process
Biological Processubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transmembrane E3 ubiquitin-protein ligase 1
  • EC number
  • Alternative names
    • RING-type E3 ubiquitin transferase TUL1

Gene names

    • Name
      TUL1
    • ORF names
      YKL247
    • Ordered locus names
      YKL034W

Organism names

Accessions

  • Primary accession
    P36096
  • Secondary accessions
    • D6VXQ1

Proteomes

Organism-specific databases

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain27-398Lumenal
Transmembrane399-419Helical
Topological domain420-431Cytoplasmic
Transmembrane432-452Helical
Topological domain453-458Lumenal
Transmembrane459-479Helical
Topological domain480-523Cytoplasmic
Transmembrane524-544Helical
Topological domain545-553Lumenal
Transmembrane554-574Helical
Topological domain575-602Cytoplasmic
Transmembrane603-623Helical
Topological domain624-635Lumenal
Transmembrane636-656Helical
Topological domain657-758Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-26
ChainPRO_000005640927-758Transmembrane E3 ubiquitin-protein ligase 1

Proteomic databases

Interaction

Subunit

Component of the DSC E3 ligase complexes composed of at least TUL1, DSC2, DSC3, UBX3, CDC48 as well as VLD1 for the vacuole-localized complex or GLD1 for the Golgi/endosome-localized complex (PubMed:25078903, PubMed:29355480).
Interacts with UBC4 (PubMed:11788821).

Complex viewer

View interactors in UniProtKB
View CPX-1190 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for zinc finger.

TypeIDPosition(s)Description
Zinc finger699-752RING-type; atypical

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    758
  • Mass (Da)
    87,880
  • Last updated
    1994-06-01 v1
  • Checksum
    F29A61E994F96628
MEIDGNTLVFIIVILFLFFSSPGGDGVSSQYEFNQLQRLKQQFRTEHNTFVNMTYTDSFRNITGLKLSYQDMLNNPLQNATYPLPGKDYDRWFPNQNYMVLPNDVIEAINTEVWNTSNDDASNLFPPNITSTLLGKIDLVSNNKYEKIRMPVPRFYEPATDFSEDIPPEGETYWSEWPSYGELHNVSFQHGEIAIQISHMSNLQDNNNYLRRNFINKKNDRWKLLNLQIDFSDKAEKEKHSIYSKAVYDIQRGRILSISQSSKFHSLFALPHYMSFQNDYNEKIFNDVKELVDEFWNFTDYTDVMTMKDVQDAYNNANFKCEYLIFLQLEPWNQYTRDQIKLIDDELNWPLGRPANLSSLPPINVVSGLLYSPDCGVRLGLHNVKGTRYELKIMSIRKHLLFGIALFAAQIYLLLTQMHHTNTPSMVNKISFYCFSMINLVDGSLATLYFVAASVVPELYLPLVISAFSCFILASIFEIRYLISIYASQVNEQNVGIINLLRGNTGTYDENRPRPAFIPDEGSIGGSLYGRFFFMLIIFTFLILSSTSWPRQLRMVFEYILIFILNSYWIPQIFRNAVKGIPSRRERARSSIGGNRSQNKMPLLWSFVIGTTIIRSLPVVYVFTYSSNVFRHHKDVHFVVFLSLWLLFQISILYSQDVLGSRWFLPKHTIPDGYSYFKPLSNEYISEHGGGTAEHTVDCAICMSDVPIYIEEIPETHKVDQHSYMVTPCNHVFHTSCLENWMNYKLQCPVCRSPLPPL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X71622
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
X69584
EMBL· GenBank· DDBJ
CAA49298.1
EMBL· GenBank· DDBJ
Genomic DNA
Z28034
EMBL· GenBank· DDBJ
CAA81869.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006944
EMBL· GenBank· DDBJ
DAA09121.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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