P36088 · FRMSR_YEAST
- ProteinFree methionine-R-sulfoxide reductase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids180 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the reversible oxidation-reduction of the R-enantiomer of free methionine sulfoxide to methionine. Does not act on S-enantiomer of free methionine sulfoxide or R-enantiomer of dabsylated methionine sulfoxide. Involved in protection against oxidative stress.
Miscellaneous
Present with 2610 molecules/cell in log phase SD medium.
Catalytic activity
- [thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (R)-S-oxide
RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:15377 + CHEBI:57844 = RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3+ CHEBI:58773
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
230 μM | free methionine-R-sulfoxide |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
443 nmol/min/mg |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | L-methionine-(R)-S-oxide reductase activity | |
Biological Process | cellular response to oxidative stress |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFree methionine-R-sulfoxide reductase
- EC number
- Short namesfRMsr
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP36088
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Single deletion of fRMsr has complete growth inhibition on methionine-R-sulfoxide medium and fRMsr and MXR1 double deletion completely blocks the growth on both methionine-R-sulfoxide and methionine-S-sulfoxide medium. FRMsr and MXR2 double deletion has no effect on growth on methionine-S-sulfoxide medium. Single mutant or any of the double mutants show no growth defects in methionine medium, even the fRMsr, MXR1 and MXR2 triple deletion mutant is viable and grows similarly to wild-type. Single deletion of fRMsr has an increased sensitivity to oxidative stress and a decreased life span of 18% compared to wild-type. FRMsr and MXR1, as well as fRMsr and MXR2 double mutants, and fRMsr, MXR1 and MXR2 triple mutant show 20% reduction in life span compared with wild-type cells.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 91 | Dramatic reduction in enzyme activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 101 | Dramatic reduction in enzyme activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 125 | Dramatic reduction in enzyme activity. | ||||
Sequence: C → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000171552 | 1-180 | Free methionine-R-sulfoxide reductase | |||
Sequence: MGSSTGFHHADHVNYSSNLNKEEILEQLLLSYEGLSDGQVNWVCNLSNASSLIWHAYKSLAVDINWAGFYVTQASEENTLILGPFQGKVACQMIQFGKGVCGTAASTKETQIVPDVNKYPGHIACDGETKSEIVVPIISNDGKTLGVIDIDCLDYEGFDHVDKEFLEKLAKLINKSCVFK |
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 99-177 | GAF | ||||
Sequence: GVCGTAASTKETQIVPDVNKYPGHIACDGETKSEIVVPIISNDGKTLGVIDIDCLDYEGFDHVDKEFLEKLAKLINKSC |
Sequence similarities
Belongs to the free Met sulfoxide reductase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length180
- Mass (Da)19,734
- Last updated1994-06-01 v1
- Checksum911158E8C0F2B0A9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X75780 EMBL· GenBank· DDBJ | CAA53405.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z28069 EMBL· GenBank· DDBJ | CAA81906.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006944 EMBL· GenBank· DDBJ | DAA09087.1 EMBL· GenBank· DDBJ | Genomic DNA |